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2 results about "Reverse Transcription Process" patented technology
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In biology, the process in cells by which an enzyme makes a copy of DNA from RNA. The enzyme that makes the DNA copy is called reverse transcriptase and is found in retroviruses, such as the human immunodeficiency virus (HIV). Reverse transcription can also be carried out in the laboratory.
Kit and method for detecting rna at room temperature
ActiveCN115820809Bmild reaction conditionslow backgroundMicrobiological testing/measurementDNA/RNA fragmentationBiochemistrySignal amplification
The present application provides a kit and method for detecting RNA at room temperature. The kit for detecting RNA at room temperature comprises: a target RNA recognition and amplification component and a double signal amplification component, and the further amplification and reporting of the signal are completed by coupling the two components, and the high-sensitivity detection of RNA is realized. The method for detecting RNA at room temperature of the present application does not need a complex reverse transcription process, can reduce the complexity of the reaction and the cost of the instrument, and has high detection efficiency.
Owner:WUHAN LIPU MEDICAL TECH CO LTD
In situ sequencing of RNA transcripts with non-uniform 5′ ends
Disclosed herein are methods for performing in situ sequencing of RNA transcripts with non-uniform 5′ ends. During reverse transcription (RT) of RNA transcripts, RT enzyme is induced to “template-switch” to a separate oligonucleotide provided as the template for the upstream flanking region. This flanking region is grafted onto the beginning of the cDNA, enabling padlock probe detection, rolling circle amplification, and fluorescent in situ sequencing. Overall, the disclosed method for in situ sequencing can be applicable for analyzing exogenously introduced transcripts (e.g., identifying and determining impact of a perturbation including a CRISPR perturbation or shRNA / siRNA / ASO perturbation), analyzing naturally occurring transcripts (e.g., measuring gene expression, detecting splicing events), and analyzing modified, naturally occurring transcripts (e.g., detecting mutations or gene edits).
Owner:INSITRO INC