54results about How to "Short preprocessing time" patented technology

The invention discloses a pretreatment method for a liquid sample in a laser-induced breakdown spectrum detection technology. The pretreatment method comprises the following steps: (1), fetching a liquid sample and removing insoluble impurities; (2), adding a precipitator and uniformly mixing; (3), filtering the liquid sample treated by the step (2) by a microfiltration membrane with an aperture not larger than 0.2 micrometer, and removing excessive liquid on the filtration membrane to obtain a filtration membrane. According to the invention, precipitation enrichment is carried out on heavy metal in the liquid sample by the precipitator, the enrichment capability of the heavy metal is greatly improved, the sampling quantity of the liquid sample is reduced, detection can be carried out by only 1ml of sampling quantity, the detection sensitivity and accuracy are high, the detection limit can reach 10-1 to 10 ppb, and various metal elements can be simultaneously detected. Meanwhile, the pretreatment method also has the advantages of short pretreatment time, simple step, low cost and the like and has an excellent application prospect.

The invention discloses a method for improving the slurryability of sludge coal-water slurry by using ultrasonic waves to crack sludge flocs. The method first uses ultrasonic waves to modify the sludge, and utilizes the shearing effect and high-temperature and high-pressure environment damage generated by ultrasonic cavitation Sludge floc structure and microbial cell wall in sludge, reduce sludge particle size, release part of interstitial water, and then mix modified sludge with coal, pulping additives, water, etc. to prepare sludge coal-water slurry, or The modified sludge is mixed into the finished coal-water slurry, and stirred evenly to make the sludge-coal-water slurry. Compared with the prior art, the present invention can significantly reduce the viscosity of sludge coal-water slurry, increase its slurry concentration, and obtain sludge coal-water slurry with excellent slurryability, and has the characteristics of short processing time and convenient operation. , suitable for large-scale promotion and application.

The invention discloses an indoor gorgon euryale planting method. The indoor gorgon euryale planting method comprises the steps of seed germination accelerating, planting, management after budding, harvesting and storage and package. According to the indoor gorgon euryale planting method, seeds are subjected to mechanical, hormone and low-temperature treatment, the time is short, germination is quick, and the germinating rate is up to 95.6%. The growing conditions of gorgon euryale are strictly controlled at different stages, the gorgon euryale can be planted all year around, and the shortcoming that one-time harvesting can be only performed each year in traditional planting is overcome; indoor cultivation is adopted, the environment is clean and tidy, a small amount of water is changed every day to keep water fresh and free of weeds, a traditional Chinese medicine disinfection bag is soaked in a pond to inhibit gorgon euryale diseases and insect pests, manpower and material resources are saved, pesticide residues are avoided, and edible safety is ensured. Low-temperature baking and drying are adopted, the drying speed is high, the bright color of the gorgon euryale is kept, nutritional loss is small, moisture regaining does not occur, the gorgon euryale is easy to store, the cool nature of the gorgon euryale can be removed through repeated drying to make drug properties slightly mild, a health-care effect is improved, and the gorgon euryale is beneficial to digestion and absorption and also reinforces the spleen and kidney.

The invention discloses an immunoaffinity column for detecting vomitoxin and application of the immunoaffinity column. The immunoaffinity column comprises a solid-phase extraction column hollow column (1), an upper sieve plate (2), an upper cover (3), a lower cover (4), a solid-phase medium (5) and a lower sieve plate (6), wherein the solid-phase medium is coupled with a vomitoxin antibody. The invention further provides a method for detecting vomitoxin residues in grains and feed by adopting the immunoaffinity column for detecting vomitoxin. The immunoaffinity column disclosed by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening a lot of samples and monitoring in the field.

The invention relates to a sample preparation method used for determining abundance of a <15>N isotope of nitrite. The sample preparation method comprises the following steps: loading a sample, namelyloading a <15>N-labeled to-be-prepared sample and aqueous solution of a reducing agent into a first bulb of a double bulb reaction tube, and loading an acidic material into a second bulb of the double bulb reaction tube; preparing a <15>NO gas, namely maintaining vacuum degree, and adding the acidic material in the second bulb into the first bulb, so that the <15>NO gas is generated; detecting, namely after the vacuum degree of a pipeline between a vacuum plug valve and a gas isotope ratio mass spectrometer is reduced to be below 2*10<-5>Pa, opening the vacuum plug valve, and introducing the<15>NO gas in the first bulb into the gas isotope ratio mass spectrometer; setting detection parameters of the gas isotope ratio mass spectrometer, detecting a relative intensity when mass numbers are30 and 31, and performing a parallel test for multiple times; and calculating an abundance value of the <15>N isotope. Compared with the prior art, the sample preparation method provided by the invention has the advantages that an operation process is safe and reliable, sample pretreatment time is greatly shortened, economic cost is low and test data accuracy and precision are high.

The invention relates to a processing method for intensifying enzymolysis saccharifying of lignocelluloses. The method comprises the following steps: respectively screening oil tea cake and lignocelluloses biomass and then drying; mixing the dried oil tea cake and lignocelluloses biomass in a mass ratio of 1:(10-20), thereby acquiring a mixture A; mixing the mixture A with deionized water, thereby acquiring a reaction solution; performing ultrasonic treatment on the reaction solution; after ending the ultrasonic treatment, cooling to room temperature and separating supernate from solid residue; removing water from the supernate and mixing the residual substance with the dried solid residue, thereby acquiring a mixture B; adding citric acid-sodium citrate buffer solution into the mixture B; uniformly mixing and then adding lignocelluloses; performing enzymatic hydrolysis for 25-48 h at 30-40 DEG C, thereby completing the treatment of the enzymolysis saccharifying of lignocelluloses. According to the invention, the ultrasonic wave and the oil tea cake are combined for preprocessing the lignocelluloses so as to accelerate the enzymolysis saccharifying of lignocelluloses, intensify the activity of the lignocelluloses, save enzyme capacity and increase the enzymolysis efficiency.

The invention discloses an ultraviolet photocatalytic pretreatment method for refractory biomass. Including biomass structure treatment and tempering treatment, the prepared biomass suspension or wastewater concentrate is added to the ultraviolet photocatalytic reactor, then 1‑10g / L oxidant is added, and 0.1‑0.8MPa compressed air is introduced for exposure Gas stirring, and then microwave excitation electrodeless ultraviolet lamp to generate ultraviolet light, under the synergistic effect of microwave, ultraviolet light, catalyst and oxidant, carry out catalytic pretreatment on biomass suspension or biomass in wastewater. In the present invention, multiple functions such as catalytic cracking, photocatalytic oxidation, and homogeneous catalytic oxidation occur simultaneously in the microwave electrodeless ultraviolet photocatalyst system, and the reaction efficiency is high, and the oxidation stage can be controlled by controlling the pretreatment conditions, so that the treated biomass is suitable for The use of microorganisms is green and environmentally friendly.

The invention discloses an extraction method of flavor components of rapeseed oil, and belongs to the field of volatile flavor substance extraction. The invention provides the method for extracting volatile flavors of rapeseed oil by coupling probe type ultrasonic-assisted extraction and solvent extraction flavor evaporation combined technology. The method has the characteristics of non-thermal effect, low energy consumption, high efficiency, real extracted substances and the like, can obviously shorten the pretreatment time of the sample, and can identify more volatile components under the same qualitative conditions compared with solid-phase micro-extraction. The method for detecting the volatile components in the rapeseed oil, provided by the invention, is simple, efficient, green and environment-friendly, and has great application potential and economic value in the fields of rapeseed oil flavor component identification, evaluation and the like.

The invention belongs to the technical field of lignocellulose pretreatment, in particular to an FeOCl-based lignocellulose pretreatment system and application thereof. The pretreatment system consists of the following effective components including 0.2-3.5 g / L of FeOCl and 0.09-1.55 mol / L of peralcohol, wherein, the peralcohol is any one of hydrogen peroxide and sodium peroxide or a mixture of the hydrogen peroxide and the sodium peroxide in an arbitrary proportion; and the pH value of the pretreatment system is 3-8. The pretreatment system can have a Fenton-like catalytic reaction within thepH value range of 3-8; the treatment time is short; and the degradation efficiency of lignin and cellulose in a lignocellulose raw material is effectively improved.

The invention discloses a liquid sample pretreatment method in laser-induced breakdown spectroscopy detection technology, which comprises the following steps: (1) taking a liquid sample and removing insoluble impurities; (2) adding a precipitating agent , and mix uniformly; (3) filter the liquid sample treated in step (2) through a microporous filter membrane with a pore size ≤ 0.2 μm, and remove excess liquid on the filter membrane to obtain a filter membrane. The present invention uses a precipitant to precipitate and enrich heavy metals in liquid samples, which greatly improves the ability to enrich heavy metals and reduces the sampling volume of liquid samples. The sampling volume can be detected by only 1ml, and the detection sensitivity and accuracy High, its detection limit can reach 10‑1~10ppb, and it can detect multiple metal elements at the same time; it also has the advantages of short pretreatment time, simple steps, low cost, etc., and has a good application prospect.

The invention discloses a method for extracting active ingredients of cistanche by adjusting osmotic pressure, which comprises the following steps: alternately spraying saline water and ethanol solution onto cistanche, transferring into a vacuum tank for vacuum treatment to break walls, soaking the cistanche subjected to wall breaking in ethanol for a period of time, grinding into thick liquid, and then carrying out ultrasonic oscillation on the slurry, filtering, purifying and drying to obtain the cistanche deserticola extract. According to the method disclosed by the invention, through alternate pretreatment of the saline water and the ethanol solution and vacuum wall breaking treatment, effective components in the cistanche are more easily extracted, and the extraction rate of the effective components of the cistanche is improved. Subsequent impurity removal treatment is carried out, so that the purity of the cistanche extract is ensured, and the cistanche extract with good quality can be obtained.

The invention relates to a method for quantitatively detecting a content of an antiviral drug in a plasma sample. The method comprises the following steps: (1) collecting trace peripheral blood; (2) mixing the peripheral blood whole blood sample with the protein precipitation solution containing an interior label, carrying out vortex and centrifugation, and diluting the supernatant to obtain a to-be-detected solution; and (3) detecting and analyzing the to-be-detected solution by adopting a high performance liquid chromatography-tandem mass spectrometry system. The invention provides the method for simultaneously detecting the vitamin A, the vitamin D2, the vitamin D3 and the vitamin E in the peripheral blood sample, the pretreatment is simple, the detection cost is low, and the detection efficiency is high.

The invention relates to a pyromellitic dianhydride detection method based on Raman spectrum, which comprises the following steps: weighing 1 + / -0.05 g of pyromellitic dianhydride sample and dissolving in 100mL of anhydrous organic solvent to prepare a high-content pyromellitic dianhydride solution; performing Raman spectrum detection on the high-content pyromellitic dianhydride solution; origin integral is adopted to open Raman spectrum data, characteristic peak height or peak area data is fitted through a Gaussian function or a Lorentz function, a pyromellitic dianhydride Raman data model is established, and the pyromellitic dianhydride content in a sample is calculated through the pyromellitic dianhydride Raman data model. The method can avoid hydrolysis of pyromellitic dianhydride in the detection process, can directly detect the content of pyromellitic dianhydride, and is short in pretreatment time and simple to operate.

The invention relates to a method for extracting desoxyribonucleic acid in edible oil, which comprises the following steps of: adding desoxyribonucleic acid extract into the edible oil, and separating out an aqueous phase containing desoxyribonucleic acid after ultrasonic emulsification; and then adding a desoxyribonucleic acid precipitant into the aqueous phase, and precipitating the desoxyribonucleic acid. Because DNA in a sample is enriched by low-energy ultrasonic emulsification in the extracting process, the pretreatment time of the sample is shortened, time consumption is short, the extraction efficiency of the DNA is improved, cross contamination between the samples to be detected is avoided, and the extraction result is more accurate and reliable.