Method for building multi-sample full-length transcriptome mixed library

Abstract

The invention relates to a method for building a multi-sample full-length transcriptome mixed library. In the reverse transcription process, primers with identification labels are adopted for reverse transcription of mRNA in different samples respectively to synthesize cDNA, and then the cDNA from different samples is mixed and processed to obtain the library. By adding a specific label sequence to each reverse transcription primer, a reverse transcription product is provided with a special label. After reverse transcription and subsequence PCR amplification are conducted on different samples with the primers with different label sequences, people can identify the reverse transcription products and which sample a certain PCR product comes from originally through the labels, and therefore mixed sample library building can be achieved.

Description

technical field [0001] The present invention relates to the technical field of molecular biology, and more particularly, relates to a method for constructing a multi-sample full-length transcriptome mixed library. Background technique [0002] Transcriptome sequencing (Transcriptomesequencing) mainly uses high-throughput sequencing to study the mRNA transcribed from a specific tissue or cell at a certain period. Transcriptome sequencing can comprehensively and quickly obtain almost all transcripts and gene sequences of a specific cell or tissue of a species in a certain state, which can be used to study gene structure and gene function, alternative splicing and prediction of new transcripts, etc. [0003] Due to the characteristics of short read length, the traditional second-generation transcriptome sequencing needs to be interrupted when building a library, and it is impossible to directly obtain all sequences from 5ˊ to 3ˊ of a single RNA molecule. The full-length transc...

AI Technical Summary

Problems solved by technology

[0004] The existing full-length transcript library does not support the multi-sample mixed library construction process. Even if the mixed sample library is built, the samples are only mixed together, and the sequencing data cannot be effectively distinguished.

In the second-generation transcriptome sequencing technology, it is possible to carry out multi-sample mix

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