Kit for detecting H4N6 avian influenza virus

A bird flu virus and kit technology, applied in the direction of microbe-based methods, microbiological measurement/testing, microbes, etc., can solve the problems of lack of simple and rapid detection methods, achieve high sensitivity, simple operation, and improve sensitivity

Inactive Publication Date: 2013-01-16
天津朝海科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The H4N6 avian influenza virus has a great impact on human life, but there is currently no simple, fast, easy-to-use and low-cost detection method for the H4N6 avian influenza virus

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  • Kit for detecting H4N6 avian influenza virus

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Embodiment Construction

[0022] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of the H4N6 avian influenza virus, and the other tube is 250 μl of a solution that can amplify the DNA in the sample to be detected. H4N6 avian influenza virus DNA or a loop-mediated isothermal nucleic acid amplification system that amplifies H4N6 avian influenza virus RNA in a sample to be detected after a reverse transcription process.

[0023] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.

[0024] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol reac...

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Abstract

The invention relates to a kit for detecting deoxyribonucleic acid (DNA) of a product which is obtained after DNA of H4N6 avian influenza virus is subjected to amplification by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. According to the kit, the loop-mediated isothermal nucleic acid amplification technology and a biological nano technology are combined to improve the sensitivity of biomolecule detection, so that the kit is operated easily, quickly and accurately without special instruments and equipment and can be widely applied to the highly-sensitive detection of H4N6 avian influenza virus in fields of family diagnosis, clinical diagnosis, the control of infectious diseases, environmental monitoring, inspection and quarantine, biotechnology and the like. The kit comprises the following two parts: (1) the nano gold particles on which a molecular probe which can identify a specific sequence of the H4N6 avian influenza virus is marked; and (2) a loop-mediated isothermal nucleic acid amplification system which can perform amplification on the DNA of the H4N6 avian influenza virus in a sample to be detected or perform the amplification on ribonucleic acid (RNA) of the H4N6 avian influenza virus in the sample to be detected after the reverse transcription process is performed.

Description

technical field [0001] The invention relates to a kit for detecting H4N6 avian influenza virus DNA or RNA amplified by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. Background technique [0002] The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for the early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr. Kary B. Mullis of PE Company invented the polymerase chain reaction (Polymerase Chain Reaction), ref...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 不公告发明人
Owner 天津朝海科技有限公司
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