Kit for detecting avian H3N8 influenza virus

An avian influenza virus and kit technology, which is applied in the directions of microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve the problems of lack of simple and rapid detection methods, and achieve the effects of high sensitivity, simple operation and low cost.

Inactive Publication Date: 2013-01-02
天津朝海科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The H3N8 avian influenza virus has a great impact on human life, but there is currently

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  • Kit for detecting avian H3N8 influenza virus

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Embodiment Construction

[0022] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of the H3N8 type avian influenza virus, and the other tube is 250 μl of a solution that can amplify the DNA in the sample to be detected. H3N8 avian influenza virus DNA or a loop-mediated isothermal nucleic acid amplification system that amplifies H3N8 avian influenza virus RNA in a sample to be detected after a reverse transcription process.

[0023] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.

[0024] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol...

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Abstract

The invention relates to a kit for detecting DNA (deoxyribonucleic acid) of an amplification product obtained by carrying out loop-mediated isothermal nucleic acid amplification technology on avian H3N8 influenza virus DNA by using nanogold particles, belongs to the technical field of biological detection. By combining loop-mediated isothermal nucleic acid amplification technology and biological nano technology, the invention enhances the biological molecule detection sensitivity, and is a method which is simple, quick and accurate to operate and does not need special instruments or equipment; and the invention can be widely used in high-sensitivity avian H3N8 influenza virus detection in the fields of household diagnosis, clinical diagnosis, infectious disease control, environmental monitoring, quarantine inspection, biotechnology and the like. The kit provided by the invention comprises the following two parts: (1) nanogold particles labeled with molecular probes capable of identifying specific sequence of avian H3N8 influenza virus; and (2) a loop-mediated isothermal nucleic acid amplification system capable of amplifying avian H3N8 influenza virus DNA in a sample to be detected or capable of amplifying avian H3N8 influenza virus DNA in the sample to be detected after a reverse transcription process.

Description

technical field [0001] The invention relates to a kit for detecting H3N8 avian influenza virus DNA or RNA amplified by a loop-mediated isothermal nucleic acid amplification technology by using nano-gold particles, and belongs to the technical field of biological detection. Background technique [0002] The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for the early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr. Kary B. Mullis of PE Company invented the polymerase chain reaction (Polymerase Chain Reaction), ref...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 不公告发明人
Owner 天津朝海科技有限公司
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