48 results about "Bio nanotechnology" patented technology

The invention relates to a pH-sensitive reduction responsive nanogel, which comprises a disulfide-bond-crosslinked sodium alginate derivate serving as an active ingredient. A preparation method of the nanogel comprises the following steps of: adding periodate into a sodium alginate aqueous solution according to molar ratio of the glycosyl unit of the sodium alginate aqueous solution to the periodate as 1:(0.01-10), and reacting away from light to obtain dialdehyde sodium alginate; stirring and uniformly mixing a dialdehyde sodium alginate aqueous solution and a 4-aminothiolphenol ethanol solution according to the molar ratio of the aldehyde group of the dialdehyde sodium alginate to the amino group of the 4-aminothiolphenol as 1:(0.01-10) away from light at the temperature of 0-25 DEG C, and then adding sodium borohydride to obtain sulphydryl sodium alginate; and self-assembling the sulphydryl sodium alginate in an aqueous solution and oxidizing the sulphydryl sodium alginate by oxygen in the solution to obtain the nanogel. The nanogel prepared by the invention has stability, pH sensitivity and reduction responsiveness and has a potential application value in the fields of biomedical implants, biological nanotechnology, drug delivery system and the like.

The present invention relates to the combination of biologic soil repairing technique with nano-class biological fertilizer, which can make soil dynamically be balanced, promote growth of microbes, improve soil, and remove the residual herbicide.

The invention relates to a preparation method for extracting a fluorescent graphene quantum dot material from coffee-ground solid waste. According to the preparation method, the coffee-ground solid waste which is low in price and easy to obtain is selected as a carbon source, and a large number of water-soluble graphene quantum dots can be obtained through a simple hydrothermal process. The synthesized quantum dots can be stably dispersed in water, are light yellow under low concentration and are brown under high concentration (that is, the higher the concentration is, the darker the color is). The particle size of the obtained graphene quantum dots ranges from 3 nm to 7 nm, the surfaces of the graphene quantum dots are provided with a large number of carboxyl groups and amino groups, subsequent surface functionalization is facilitated, multi-purpose application can be brought, and meanwhile the excellent fluorescent property is exerted. The synthesized quantum dot material shows the attractive application prospect in the high and new technical fields such as environmental protection, bionanotechnology, new energy resources and nanometer devices, and the synthesizing method is simple, environmentally friendly, rapid, low in energy consumption and suitable for industry expanding.

The invention relates to a photocrosslinking nano hydrogel which comprises the effective component of an ultraviolet photo-initiated azido crosslinking carboxymethyl chitosan derivative, and is prepared by the method comprising the following steps: taking the water solution of carboxymethyl chitosan; adding the organic solution of 4-azidobenzaldehyde under the condition that the mol ratio of the sugar moiety of the carboxymethyl chitosan to the 4-azidobenzaldehyde is 1:(0.01-10); stirring and mixing evenly at 0-30 DEG C under the dark condition, wherein when a photocrosslinking carboxymethyl chitosan amphiphilic polymer is generated by the reaction, the amphiphilic polymer can be self-assembled into a photocrosslinking nano-particle mixed solution; and then radiating the nano-particle mixed solution by ultraviolet light to initiate azido crosslinking. The invention has simple process and easy operation, can obtain photocrosslinking targeted nano gel by coupling carboxyl with a target molecule or a target molecule derivative, and has potential application value in the fields of tissue engineering, biomedical implants, biological nano technology, drug delivery systems, and the like.

The present invention discloses a female healthcare food and a preparation method thereof. The female healthcare food comprises the following raw materials in parts by weight: 30-60 parts of lepidium meyenii, 20-55 parts of kudzu vine root and 10-20 parts of Chinese wolfberry fruits. According to the preparation method, biological health activity molecules are extracted through a modern Chinese medicine technology, and the extracted biological healthcare active molecules are added to edible materials and prepared into tablets, granules, hard capsules or oral solutions. The lepidium meyenii, the kudzu vine root and the Chinese wolfberry fruits are processed by a bionanotechnology, such that bioavailability of the healthcare active molecules can be improved, and a female healthcare food can be prepared by a fine processing. The prepared female healthcare food is safe, effective, non-toxic and harmless. The female healthcare food has collaborative action for improving sexual function, improving fertility, regulating endocrine, reducing bone rarefaction caused by ovarian function decline and other related diseases, and improving immune function.

The invention discloses a microfluidic paper based sensor based on fluoroimmunoassay and utilizing a quantum dot as a marker to detect norfloxacin (NOR) in milk and belongs to the technical field of biological nanometer. A preparation method of the sensor comprises the steps: firstly, forming a hydrophilic area and a hydrophobic area on chromatographic paper through a wax spray printing technology, roasting for a certain time and performing plasma treatment on the paper; then, preparing NOR-bovine serum albumin (BSA) coating antigen and a quantum dot marked NOR antibody; then, respectively adding the coating antigen and a goat anti-mouse secondary antibody into two white paper areas in parallel up and down and adding an NOR and quantum dot marked antibody mixture into a coating layer, folding the paper and permeating the solution into a secondary antibody area; finally, utilizing immunoassay determination to finally quantitatively detect the norfloxacin in the milk through fluorescent signals. The microfluidic paper based sensor disclosed by the invention has the advantages of low cost, simpleness in operation, high flexibility, low detection limit and quickness in detecting NOR.

The invention discloses a paper-based electrochemical analysis device and method based on single-layer Mxene modification, and belongs to the technical field of biological nanotechnology. The sensor comprises a detection layer A and a sample adding layer B. The detection layer A is used for detecting cardiac troponin cTnI. The manufacturing method comprises the following steps: firstly, spraying wax and printing paraffin on whatman paper, and performing baking for a certain period of time to form a hydrophilic region and a hydrophobic region; secondly, performing silk-screen printing of a carbon working electrode on the detection layer A, and performing silk-screen printing of a carbon counter electrode and a silver-silver chloride reference electrode on the sample adding layer B; adding the MXene to the working electrode of the detection layer A again, then adding the cTnI antibody to the working electrode of the detection layer A, and adding the sample to the layer B for folding. Andfinally, adding a detection sample into the sample adding layer B, performing determining by utilizing immunoassay, and finally quantitatively detecting cTnI in the sample through an electrochemicalsignal.

The invention relates to a kit for detecting deoxyribonucleic acid (DNA) of a product which is obtained after DNA of H4N6 avian influenza virus is subjected to amplification by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. According to the kit, the loop-mediated isothermal nucleic acid amplification technology and a biological nano technology are combined to improve the sensitivity of biomolecule detection, so that the kit is operated easily, quickly and accurately without special instruments and equipment and can be widely applied to the highly-sensitive detection of H4N6 avian influenza virus in fields of family diagnosis, clinical diagnosis, the control of infectious diseases, environmental monitoring, inspection and quarantine, biotechnology and the like. The kit comprises the following two parts: (1) the nano gold particles on which a molecular probe which can identify a specific sequence of the H4N6 avian influenza virus is marked; and (2) a loop-mediated isothermal nucleic acid amplification system which can perform amplification on the DNA of the H4N6 avian influenza virus in a sample to be detected or perform the amplification on ribonucleic acid (RNA) of the H4N6 avian influenza virus in the sample to be detected after the reverse transcription process is performed.

The invention provides a preparation method of hyperconcentration placental-peptide nanometer viable-cell freeze-dried powder, and a hyperconcentration placental-peptide nanometer viable-cell freeze-dried capsule which comprises the hyperconcentration placental-peptide nanometer viable-cell freeze-dried powder prepared by the preparation method. The preparation method adopts the CO2 supercritical extraction, the ultra-low-temperature freeze-drying technology, and the biological nanotechnology. The hyperconcentration placental-peptide nanometer viable-cell freeze-dried capsule is obtained by utilizing the lipidosome preparation technology.

The invention relates to a kit for detecting product DNA of hepatitis B virus DNA amplified through a loop-mediated isothermal nucleic acid amplification technology by using gold nanoparticles, belonging to the technical field of biological detection. In combination with the loop-mediated isothermal nucleic acid amplification technology and a biological nanotechnology, the invention has the advantages that the sensitivity of biological molecular detection is improved, the operation of the method is simple, the detection is rapid, the accuracy is high, special instruments and devices are not required, and the kit can be widely used for high-sensitivity hepatitis B virus detection in fields such as family diagnosis, clinical diagnosis, infectious disease control, environmental monitoring, inspection and quarantine, biotechnology and the like. The kit comprises: (1) gold nanoparticles labeled with molecular probes capable of identifying specific sequences of hepatitis B viruses; and (2) a loop-mediated isothermal nucleic acid amplification system capable of amplifying hepatitis B virus DNA in a sample to be detected or capable of amplifying hepatitis B virus RNA in the sample to be detected after reverse transcription.

The invention relates to a kit for detecting product deoxyribonucleic acid (DNA) which is amplified from coxsackie virus A16 subjected to a loop-mediated isothermal amplification technology by using nano-gold particles, belonging to the technical field of biological detection. By combining the loop-mediated isothermal amplification technology and biological nanometer technology, the sensitivity of biological molecular detection is improved, so the invention is a method with simplicity in operation, quickness and accuracy without professional instrument and equipment, and the kit can be widely applied to high sensitivity coxsackie virus A16 detection in the fields of household diagnosis, clinical diagnosis, infectious disease control, environmental monitoring, inspection and quarantine, biological technology and the like. The kit comprises two parts: (1) nano-gold particles marked with molecular probes which can identify the specific sequence of the coxsackie virus A16; and (2) a loop-mediated isothermal amplification system which can amplify the DNA of the coxsackie virus A16 in a sample to be detected, or amplify ribonucleic acid (RNA) of the coxsackie virus A16 in the sample to be detected after a reverse transcription process.

The invention discloses a healthcare maca oral solution and a preparation method thereof. The healthcare maca oral solution is prepared from the following raw materials in parts by weight: 40 to 80 parts of maca, 20 to 40 parts of kudzuvine root, 10 to 20 parts of fructus momordicae and 10 to 20 parts of barbary wolfberry fruit. Biological healthcare active molecules are extracted through a modern Chinese medicine technology and are added with edible accessories such as stevioside and D-sodium erythorbate to prepare the healthcare maca oral solution. By virtue of the maca, the kudzuvine root, the fructus momordicae and the barbary wolfberry fruit, the biological utilization of the biological healthcare active molecules is improved through a biological nanometer technology, and the healthcare maca oral solution is prepared by fine machining; the healthcare maca oral solution prepared through a formula is safe and effective to drink, non-toxic and harmless. The healthcare maca oral solution has a collaborative effect of regulating internal secretion, relieving fatigue, delaying senescence and improving a body immunity function.

The invention relates to a kit for detecting product DNA of AH1N1 influenza virus DNA amplified through a loop-mediated isothermal nucleic acid amplification technology by using gold nanoparticles, belonging to the technical field of biological detection. In combination with the loop-mediated isothermal nucleic acid amplification technology and a biological nanotechnology, the invention has the advantages that the sensitivity of biological molecular detection is improved, the operation of the method is simple, the detection is rapid, the accuracy is high, special instruments and devices are not required, and the kit can be widely used for high-sensitivity AH1N1 influenza virus detection in fields such as family diagnosis, clinical diagnosis, infectious disease control, environmental monitoring, inspection and quarantine, biotechnology and the like. The kit comprises: (1) gold nanoparticles labeled with molecular probes capable of identifying specific sequences of AH1N1 influenza viruses; and (2) a loop-mediated isothermal nucleic acid amplification system capable of amplifying AH1N1 influenza virus DNA in a sample to be detected or capable of amplifying AH1N1 influenza virus RNA in the sample to be detected after reverse transcription.

The present invention relates to purified multimeric proteins, comprising PlyCB monomers or functional fragments or variants thereof, which self-assemble into a ring like structure. The present invention also encompasses such multimeric proteins that comprise an integrin-binding sequence. The present invention further discloses contrast reagents, implant coatings, bone implants, and complexes for use in bionanotechnology applications, each of which comprise such multimeric proteins.

The invention provides a method for optimizing activity of nano-enzyme particles. Specifically, a small molecule compound containing an imidazole ring is utilized for modification to improve the affinity of the nano-enzyme particles to a substrate H2O2, and the activity of nano-enzyme particles is optimized. By means of the method, the affinity of the nano-enzyme particles to the substrate H2O2 isimproved by 12 times, and the catalytic efficiency of the nano-enzyme particles is improved by 21 times. The activity of nano-enzyme particles is optimized by simulating the active center of a natural enzyme, more novel methods can be provided for optimization of the activity of the nano-enzyme particles, and the actual application of nano-enzyme particles in the bionanotechnology field can be promoted.

The purpose of the invention is to disclose a method for producing the quick-acting mosquito bite lingling of the traditional Chinese medicine by utilizing the bionano technology. It belongs to the field of medicine. This product is a pharmaceutical composition prepared from pure natural herbal medicines through careful processing according to a certain process; it is mainly used to prevent mosquito bites and various local symptoms caused by mosquito bites. The raw materials used in the medicine of the invention have wide and easy-to-obtain sources, are low in cost, and are not easy to cause local skin allergies. The equipment used for the preparation of the composition of the invention is simple, and the preparation is simple and easy.

The invention relates to a kit for detecting product DNA by utilizing nano-gold particles after human immunodeficiency virus is amplified through a loop-mediated isothermal nucleic acid amplification technique, which belongs to the technical field of biological detection. The kit combines the loop-mediated isothermal nucleic acid amplification technique with a biological nanotechnology to improve the detection sensitivity of biological molecules, is a method with simple operation, rapidness, accuracy and no need for professional instruments and equipment, and can be widely applied to high-sensitivity HIV detection in the fields of household diagnosis, clinical diagnosis, infectious diseases control, environment monitoring, inspection and quarantine, biotechnology and the like. The kit comprises two parts, namely (1) the nano-gold particles marked with molecular robes capable of identifying specific sequences of HIV and (2) a loop-mediated isothermal nucleic acid amplification system capable of amplifying the escherichia coli DNA in to-be-detected samples or performing a reverse transcription process and then amplifying the RNA of the HIV in the to-be-detected samples.

The invention relates to a kit for detecting product DNA of Salmonella spp. DNA amplified through a loop-mediated isothermal nucleic acid amplification technology by using gold nanoparticles, belonging to the technical field of biological detection. In combination with the loop-mediated isothermal nucleic acid amplification technology and a biological nanotechnology, the invention has the advantages that the sensitivity of biological molecular detection is improved, the operation of the method is simple, the detection is rapid, the accuracy is high, special instruments and devices are not required, and the kit can be widely used for high-sensitivity Salmonella spp. detection in fields such as family diagnosis, clinical diagnosis, infectious disease control, environmental monitoring, inspection and quarantine, biotechnology and the like. The kit comprises: (1) gold nanoparticles labeled with molecular probes capable of identifying specific sequences of Salmonella spp.; and (2) a loop-mediated isothermal nucleic acid amplification system capable of amplifying Salmonella spp. DNA in a sample to be detected or capable of amplifying Salmonella spp. RNA in the sample to be detected after reverse transcription.

The invention relates to a kit for detecting DNA (deoxyribose nucleic acid) of a product of H3N2 avian influenza virus through gold nanoparticles after the product is amplified via a loop-mediated isothermal nucleic acid amplification technology, belonging to the technical field of biological detection. According to the kit, the loop-mediated isothermal nucleic acid amplification technology and a bio nanotechnology are combined, therefore, the sensitivity in detection of biomolecule is improved, and a method which can be simply and fast operated along with accuracy without professional instrument and equipment is provided; and the kit can be widely applied to detecting high-sensitivity H3N2 avian influenza virus in the fields of family diagnosis, clinical diagnosis, communicable disease control, environment monitoring, inspection and quarantine and biotechnology and the like. The kit provided by the invention includes two parts which are respectively (1) gold nanoparticles on which molecular probes for distinguishing the special sequence of the H3N2 avian influenza virus are marked, and (2) the loop-mediated isothermal nucleic acid amplification system for amplifying the N2 avian influenza DNA of a sample to be detected or amplifying the H3N2 avian influenza RNA in the sample to be detected after carrying out reverse transcription.