The invention relates to a kit for detecting deoxyribonucleic acid (
DNA) of a product which is obtained after
DNA of H4N6
avian influenza virus is subjected to amplification by a loop-mediated isothermal
nucleic acid amplification technology by using nano
gold particles, and belongs to the technical field of biological detection. According to the kit, the loop-mediated isothermal
nucleic acid amplification technology and a biological nano technology are combined to improve the sensitivity of
biomolecule detection, so that the kit is operated easily, quickly and accurately without special instruments and equipment and can be widely applied to the highly-sensitive detection of H4N6
avian influenza virus in fields of family diagnosis,
clinical diagnosis, the control of infectious diseases,
environmental monitoring, inspection and
quarantine,
biotechnology and the like. The kit comprises the following two parts: (1) the nano
gold particles on which a
molecular probe which can identify a specific sequence of the H4N6
avian influenza virus is marked; and (2) a loop-mediated isothermal
nucleic acid amplification
system which can perform amplification on the
DNA of the H4N6 avian influenza
virus in a sample to be detected or perform the amplification on ribonucleic acid (
RNA) of the H4N6 avian influenza
virus in the sample to be detected after the
reverse transcription process is performed.