Human enterovirus 71 detection kit
A technology of enterovirus and kit, applied in the field of biological detection, can solve the problems of enterovirus 71 lack of simple, fast, and easy-to-use detection methods, and achieve high sensitivity, low cost, and improved sensitivity
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[0020] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of human enterovirus 71, and the other tube is 250 μl of a solution that can amplify the sample to be detected The human enterovirus 71 DNA or the loop-mediated isothermal nucleic acid amplification system that amplifies the human enterovirus 71 RNA in the sample to be detected after a reverse transcription process.
[0021] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.
[0022] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol reaction buffer (New Engla...
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