Human enterovirus 71 detection kit

A technology of enterovirus and kit, applied in the field of biological detection, can solve the problems of enterovirus 71 lack of simple, fast, and easy-to-use detection methods, and achieve high sensitivity, low cost, and improved sensitivity

Inactive Publication Date: 2011-11-02
天津朝海科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is currently a lack of simple, rapid, easy-to-use and low-cost detection methods for human enterovirus 71

Method used

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Embodiment Construction

[0020] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of human enterovirus 71, and the other tube is 250 μl of a solution that can amplify the sample to be detected The human enterovirus 71 DNA or the loop-mediated isothermal nucleic acid amplification system that amplifies the human enterovirus 71 RNA in the sample to be detected after a reverse transcription process.

[0021] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.

[0022] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol reaction buffer (New Engla...

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Abstract

The invention relates to a kit, which utilizes nano-gold particles to detect a product DNA of a human enterovirus 71 DNA amplified by a loop-mediated isothermal nucleic acid amplification technology, and belongs to the technical field of biological detection. The invention combines a loop-mediated isothermal nucleic acid amplification technology and a bio-nano technology to in crease detection sensitivity of biomolecules, and provides a simply operated, rapid, accurate and no professional equipment required method. The method can be widely applied to high-sensitivity human enterovirus 71 detection in fields of household diagnosis, clinic diagnosis, infectious disease control, environment monitoring, examination and quarantine, and biotechnology, etc. The kit of the invention comprises two pats of (1) nano-gold particles marked with molecule probes capable of identifying human enterovirus 71 specific sequence; and (2) a loop-mediated isothermal nucleic acid amplification system capable of amplifying the DNA of human enterovirus 71 in a sample to be detected or amplifying an RNA of human enterovirus 71 in a sample to be detected after a reverse transcription.

Description

technical field [0001] The invention relates to a kit for detecting human enterovirus type 71 DNA or RNA amplified by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. Background technique [0002] The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for the early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr. Kary B. Mullis of PE Company invented the polymerase chain reaction (Polymerase Chain Reaction), refe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 孙国明
Owner 天津朝海科技有限公司
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