Kit for detecting human immunodeficiency virus

Abstract

The invention relates to a kit for detecting product DNA by utilizing nano-gold particles after human immunodeficiency virus is amplified through a loop-mediated isothermal nucleic acid amplification technique, which belongs to the technical field of biological detection. The kit combines the loop-mediated isothermal nucleic acid amplification technique with a biological nanotechnology to improve the detection sensitivity of biological molecules, is a method with simple operation, rapidness, accuracy and no need for professional instruments and equipment, and can be widely applied to high-sensitivity HIV detection in the fields of household diagnosis, clinical diagnosis, infectious diseases control, environment monitoring, inspection and quarantine, biotechnology and the like. The kit comprises two parts, namely (1) the nano-gold particles marked with molecular robes capable of identifying specific sequences of HIV and (2) a loop-mediated isothermal nucleic acid amplification system capable of amplifying the escherichia coli DNA in to-be-detected samples or performing a reverse transcription process and then amplifying the RNA of the HIV in the to-be-detected samples.

Description

A kit for detecting human immunodeficiency virus 1. Technical field The invention relates to a kit for detecting human immunodeficiency virus (HIV) product DNA amplified by loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, belonging to the technical field of biological detection. 2. Background technology The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr.KaryB.Mullis of PE Company invented the polymerase chain r...

AI Technical Summary

Problems solved by technology

There is currently a lack of simple, rapid, easy-to-use and low-cost detection methods for HIV

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