Kit for detecting H7N7 type avian influenza viruses

An avian influenza virus and kit technology, applied in the field of biological detection, can solve the problems of lack of simple and rapid detection methods, and achieve the effects of high sensitivity, simple operation and improved sensitivity

Inactive Publication Date: 2013-01-02
天津朝海科技有限公司
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Problems solved by technology

[0006] The H7N7 avian influenza virus has a great impact on human life, but there is currently no simple, fast, easy-to-use and low-cost detection method for the H7N7 avian influenza virus

Method used

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  • Kit for detecting H7N7 type avian influenza viruses

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Embodiment Construction

[0022] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of the H7N7 type avian influenza virus, and the other tube is 250 μl of a solution that can amplify the DNA in the sample to be detected. H7N7 avian influenza virus DNA or a loop-mediated isothermal nucleic acid amplification system for amplifying H7N7 avian influenza virus RNA in a sample to be detected after a reverse transcription process.

[0023] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.

[0024] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol...

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Abstract

The invention relates to a kit for detecting deoxyribonucleic acid (DNA) of a product of H7N7 type avian influenza virus DNA through gold nanoparticles after the product is amplified via a loop-mediated isothermal nucleic acid amplification technology, belonging to the technical field of bioinstrumentation. Combining with the ring mediate isothermality nucleic acid amplification technology and a biological nanometer technology, the kit is capable of improving sensitivity of biomolecule detection, is a method which is simple to operate, rapid and accurate and needs no professional equipment, and can be widely used for high-sensitivity H7N7 type avian influenza virus detection in the fields of house diagnosis, clinical diagnosis, communicable disease control, environmental monitoring, inspection quarantine and a biotechnology and the like. The kit comprises two portions including (1) the nanometer gold particles marked with molecular probes of H7N7 type avian influenza virus specific sequences and (2) the loop-mediated isothermal nucleic acid amplification system for amplifying the H7N7 avian influenza DNA of a sample to be detected or amplifying the H7N7 avian influenza RNA in the sample to be detected after carrying out reverse transcription..

Description

technical field [0001] The invention relates to a kit for detecting H7N7 avian influenza virus DNA or RNA amplified by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. Background technique [0002] The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for the early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr. Kary B. Mullis of PE Company invented the polymerase chain reaction (Polymerase Chain Reaction), ref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 不公告发明人
Owner 天津朝海科技有限公司
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