Kit for detecting H7N7 type avian influenza viruses
An avian influenza virus and kit technology, applied in the field of biological detection, can solve the problems of lack of simple and rapid detection methods, and achieve the effects of high sensitivity, simple operation and improved sensitivity
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[0022] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of a nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of the H7N7 type avian influenza virus, and the other tube is 250 μl of a solution that can amplify the DNA in the sample to be detected. H7N7 avian influenza virus DNA or a loop-mediated isothermal nucleic acid amplification system for amplifying H7N7 avian influenza virus RNA in a sample to be detected after a reverse transcription process.
[0023] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.
[0024] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol...
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