Microfluidic paper based sensor based on fluoroimmunoassay and utilizing quantum dot as marker to detect norfloxacin in milk
A fluorescence immunoassay and norfloxacin technology, applied in the field of biological nanometers, can solve the problems of complex sample processing, difficult to popularize and high cost, and achieve the effects of wide application range, high sensitivity and low cost
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Embodiment 1
[0031] A microfluidic paper-based sensor for detecting norfloxacin in milk based on fluorescent immunoassay using quantum dots as a marker, comprising the following steps:
[0032] a. Formation of plasma treated paper
[0033] 1) Design a paper-based model of a certain size, and form a hydrophilic area (such as figure 1 Shown in white area) and hydrophobic area (such as figure 1 shown in black area);
[0034] 2) Bake at 105°C for 5 minutes;
[0035] 3) Treat the paper with a plasma cleaner for 4 minutes to obtain the required paper;
[0036] 4) The plasma-treated paper needs to be sealed before adding samples to prevent oxidation.
[0037] b. Preparation of NOR-BSA coated antigen
[0038] 1) Add 11.7mgNOR to 3mL of anhydrous dimethylformamide;
[0039] 2) Add 70.5mgEDC.HCl and 21.2mgNHS;
[0040] 3) Centrifuge after stirring overnight at room temperature;
[0041] 4) The supernatant was added to 33.0 mg of BSA dissolved in 5 mL of PBS (0.01 M, pH=7.4);
[0042] 5) Aft...
Embodiment 2
[0060] A microfluidic paper-based sensor for detecting norfloxacin in milk based on fluorescent immunoassay using quantum dots as a marker, comprising the following steps:
[0061] a. Formation of plasma treated paper
[0062] 1) Design a paper-based model of a certain size, and form a hydrophilic area (such as figure 1 Shown in white area) and hydrophobic area (such as figure 1 shown in black area);
[0063] 2) Bake at 105°C for 5 minutes;
[0064] 3) Treat the paper with a plasma cleaner for 4 minutes to obtain the required paper;
[0065] 4) The plasma-treated paper needs to be sealed before adding samples to prevent oxidation.
[0066] b. Preparation of NOR-BSA coated antigen
[0067] 1) Add 11.7mgNOR to 3mL of anhydrous dimethylformamide;
[0068] 6) Add 70.5mgEDC.HCl and 21.2mgNHS;
[0069] 7) Centrifuge after stirring overnight at room temperature;
[0070] 8) The supernatant was added to 33.0 mg of BSA dissolved in 5 mL of PBS (0.01 M, pH=7.4);
[0071] 9) Aft...
Embodiment 3
[0083] A microfluidic paper-based sensor for detecting norfloxacin in milk based on fluorescent immunoassay using quantum dots as a marker, comprising the following steps:
[0084] a. Formation of plasma treated paper
[0085] 1) Design a paper-based model of a certain size, and form a hydrophilic area (such as figure 1 Shown in white area) and hydrophobic area (such as figure 1 shown in black area);
[0086] 2) Bake at 105°C for 5 minutes;
[0087] 3) Treat the paper with a plasma cleaner for 4 minutes to obtain the required paper;
[0088] 4) The plasma-treated paper needs to be sealed before adding samples to prevent oxidation.
[0089] b. Preparation of NOR-BSA coated antigen
[0090] 1) Add 11.7mgNOR to 3mL of anhydrous dimethylformamide;
[0091] 10) Add 70.5mgEDC.HCl and 21.2mgNHS;
[0092] 11) Centrifuge after stirring overnight at room temperature;
[0093] 12) The supernatant was added to 33.0 mg of BSA dissolved in 5 mL of PBS (0.01 M, pH=7.4);
[0094] 13)...
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