Nano microball of shombycin protein and preparation process thereof
A fibroin, nano-microsphere technology, applied in nanotechnology, polymer chemistry, biochemistry, protein, medicine, biology, can solve problems such as initiator residues, limited application scope, surfactant residues, etc., to achieve cost Low cost, high biosafety, simple process effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0020] Example 1: Immerse about 20g of waste silk into 1L of 0.5% NaHCO 3 solution, stirred and boiled for 45min, then removed and washed with deionized water. After repeating the above operation once, dry the silk at 65°C for later use.
[0021] Weigh 5 g of the degummed silk after the above treatment and dissolve it in 100 ml of BrLi solution with a concentration of 9.6 mol / L, and dissolve under constant temperature and low speed magnetic stirring at 45°C. The protein solution was poured into a cellulose dialysis bag, and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 4.32%.
[0022] Take 5ml of the above silk protein solution, add 1ml of methanol dropwise in a constant temperature water bath at 25°C, stabilize it for 5min, and put it into a low-temperature refrigerator for 96h at -5°C. Silk protein was frozen for 96 hours to form a milky white ice cube, and thawed at room temperature to form a milky milky whi...
Embodiment 2
[0023] Embodiment 2: About 15g waste silk is immersed in 1L 0.5%Na 2 CO 3 solution, stirred and boiled for 50 min, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 65°C for later use.
[0024] Weigh 5 g of the degummed silk after the above treatment and dissolve it in 100 ml of BrLi solution with a concentration of 10.0 mol / L, and dissolve under constant temperature and low speed magnetic stirring at 45°C. The protein solution was poured into a cellulose dialysis bag, and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 3%, and then diluted to adjust the concentration of the silk protein solution to 1.5%.
[0025] Take 10ml of the above silk protein solution, add 3ml of acetone dropwise in a constant temperature water bath at 25°C, stabilize it for 5 minutes, and put it into a low-temperature refrigerator for freezing at -20°C for 48h. Silk protein is ...
Embodiment 3
[0026] Example 3: Immerse about 20 g of waste silk into 2 L of 0.5 mol / L boric acid-borax buffer solution, stir and boil for 60 min, take it out, and wash it with deionized water. After repeating the above operation once, dry the silk at 65°C for later use.
[0027] Weigh 4 g of the degummed silk after the above treatment and dissolve it in 100 ml of BrLi solution with a concentration of 10.0 mol / L, and dissolve under constant temperature and low speed magnetic stirring at 45°C. The protein solution was poured into a cellulose dialysis bag, and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 2.0%.
[0028] Take 10ml of the above silk protein solution, add 5ml of ethanol dropwise in a constant temperature water bath at 25°C, stabilize it for 5min, and put it into a low-temperature refrigerator at -40°C for 36h. After freezing, silk protein forms milky white ice cubes, and thaws at room temperature to form a milky m...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
polydispersity index | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com