Nano microball of shombycin protein and preparation process thereof

A fibroin, nano-microsphere technology, applied in nanotechnology, polymer chemistry, biochemistry, protein, medicine, biology, can solve problems such as initiator residues, limited application scope, surfactant residues, etc., to achieve cost Low cost, high biosafety, simple process effect

Inactive Publication Date: 2005-01-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the research on the use of silk protein as a drug release carrier has just started, and the dosage form is relatively simple, mainly in the form of membrane and hydrogel
Since the silk protein solution is easily denatured under external influences such as external disturbances, it is difficult to obtain microparticles or nanoparticles by conventional methods, which greatly limits its application range.
At present, several types of natural polymer nanoparticles have been extensively studied, and there are more or less a series of problems such as antigenicity, initiator residue, surfactant residue, and the need to add cross-linking agents, which pose a potential threat to the human body.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Immerse about 20g of waste silk into 1L of 0.5% NaHCO 3 solution, stirred and boiled for 45min, then removed and washed with deionized water. After repeating the above operation once, dry the silk at 65°C for later use.

[0021] Weigh 5 g of the degummed silk after the above treatment and dissolve it in 100 ml of BrLi solution with a concentration of 9.6 mol / L, and dissolve under constant temperature and low speed magnetic stirring at 45°C. The protein solution was poured into a cellulose dialysis bag, and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 4.32%.

[0022] Take 5ml of the above silk protein solution, add 1ml of methanol dropwise in a constant temperature water bath at 25°C, stabilize it for 5min, and put it into a low-temperature refrigerator for 96h at -5°C. Silk protein was frozen for 96 hours to form a milky white ice cube, and thawed at room temperature to form a milky milky whi...

Embodiment 2

[0023] Embodiment 2: About 15g waste silk is immersed in 1L 0.5%Na 2 CO 3 solution, stirred and boiled for 50 min, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 65°C for later use.

[0024] Weigh 5 g of the degummed silk after the above treatment and dissolve it in 100 ml of BrLi solution with a concentration of 10.0 mol / L, and dissolve under constant temperature and low speed magnetic stirring at 45°C. The protein solution was poured into a cellulose dialysis bag, and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 3%, and then diluted to adjust the concentration of the silk protein solution to 1.5%.

[0025] Take 10ml of the above silk protein solution, add 3ml of acetone dropwise in a constant temperature water bath at 25°C, stabilize it for 5 minutes, and put it into a low-temperature refrigerator for freezing at -20°C for 48h. Silk protein is ...

Embodiment 3

[0026] Example 3: Immerse about 20 g of waste silk into 2 L of 0.5 mol / L boric acid-borax buffer solution, stir and boil for 60 min, take it out, and wash it with deionized water. After repeating the above operation once, dry the silk at 65°C for later use.

[0027] Weigh 4 g of the degummed silk after the above treatment and dissolve it in 100 ml of BrLi solution with a concentration of 10.0 mol / L, and dissolve under constant temperature and low speed magnetic stirring at 45°C. The protein solution was poured into a cellulose dialysis bag, and dialyzed with deionized water for three days to obtain a silk protein aqueous solution with a concentration of 2.0%.

[0028] Take 10ml of the above silk protein solution, add 5ml of ethanol dropwise in a constant temperature water bath at 25°C, stabilize it for 5min, and put it into a low-temperature refrigerator at -40°C for 36h. After freezing, silk protein forms milky white ice cubes, and thaws at room temperature to form a milky m...

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PUM

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Abstract

The invention belongs to a fully new nano microsphere and its preparing method, applying bionano technique in preparing course, making fibroin self-mount on proper conditions to form nano microsphere, without adding in any initiator, surfactant, cross-linking agent, etc in the whole preparing course, thus completely solving the problem that existing traditional carried drug nano microsphere preparing method introduces the above substances to have latent menaces to human bodies. The preparing process is simple and easy to apply, able to prepare monodisperse nano microsphere with particle size 80-2000nm, and the prepared nano microsphere has good stability and redispersion property, and can be stored for a long time.

Description

technical field [0001] The invention belongs to the fields of nanotechnology, polymer chemistry, biochemistry, protein, medicine and biotechnology, and in particular relates to a natural biomacromolecule-silk protein nano-microsphere and a preparation method thereof. Background technique [0002] Silk is one of the earliest natural proteins used by humans. It is a protein fiber composed of silk protein (70-80%) and sericin. It has been used in the textile industry as a natural fiber with excellent performance. In recent years, with the rapid development of biomedical technology, silk protein has been widely used in many fields such as health care products, medicine, biotechnology, and fine chemical industry due to its good biocompatibility, non-antigenicity, and easy degradability. , such as medical sutures, tissue engineering, medical dressings, smart hydrogels, drug-loaded slow-release films, and biosensors. The present inventors have mixed silk protein solution with urea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00C08J3/16
Inventor 邵正中曹正兵陈新
Owner FUDAN UNIV
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