Kit for detecting Salmonella spp.
A Salmonella and kit technology, applied in the determination/inspection of microorganisms, resistance to vector-borne diseases, material analysis by observing the impact on chemical indicators, etc., can solve problems such as the lack of simple detection methods for Salmonella
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[0015] The kit of the present invention includes 2 tubes of solution, one tube is 50 μl of nano gold particle solution labeled with a molecular probe that can recognize the specific sequence of Salmonella, and the other tube is 250 μl that can amplify the Salmonella DNA in the sample to be detected Or a loop-mediated isothermal nucleic acid amplification system that amplifies the Salmonella RNA in the sample to be detected after a reverse transcription process.
[0016] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20nm at a concentration of 1.2nM, and the surface of the gold nanoparticles is marked with a sulfhydryl-modified DNA probe.
[0017] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4M betaine (Sigma-Aldrich, St.Louis, MO), 10 mM MgSO 4 , 1.4mM dNTPs, 1×ThermoPol reactionbuffer (New England Biolabs, Ipswich, MA), 80 U Bst DNA polyme...
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