Microfluidic paper-based sensor for detecting cardiac troponin I based on single-layer MXene enhanced signal for electrochemical analysis

A technique for cardiac troponin and electrochemical analysis, applied in the field of biological nanometers, can solve the problems of difficult promotion, high cost, complicated sample processing, etc., and achieve the effects of wide application range, low cost and high sensitivity

Pending Publication Date: 2021-02-23
NANJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem solved by the present invention is: aiming at the deficiencies of the prior art, a microfluidic paper-based sensor based on the enhanced signal detection of cardiac troponin I with a single layer of MXene for electrochemical analysis is proposed, which solves the problem of complicated sample processing in previous detections. Disadvantages such as high cost and difficult promotion

Method used

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  • Microfluidic paper-based sensor for detecting cardiac troponin I based on single-layer MXene enhanced signal for electrochemical analysis
  • Microfluidic paper-based sensor for detecting cardiac troponin I based on single-layer MXene enhanced signal for electrochemical analysis
  • Microfluidic paper-based sensor for detecting cardiac troponin I based on single-layer MXene enhanced signal for electrochemical analysis

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Embodiment 1

[0038] A microfluidic paper-based sensor for detecting cardiac troponin I with MXene-enhanced signal based on electrochemical analysis, including the following steps:

[0039] a. Formation of plasma treated paper

[0040] 1) Design a paper-based model of a certain size, and form a hydrophilic area and a hydrophobic area through wax spray printing technology;

[0041] 2) Bake at 90°C for 5 minutes;

[0042] 3) Treat the paper with a plasma cleaner for 4 minutes to obtain the required paper;

[0043] 4) The plasma-treated paper needs to be sealed before adding samples to prevent oxidation.

[0044] b. Preparation of monolayer MXene

[0045] 1) Stir and mix 5mL water, 15mL hydrochloric acid and 1g lithium fluoride evenly;

[0046] 2) Add 1g of MXene precursor (MAX) and stir at 35°C for 24h;

[0047] 3) Centrifuge at 3500rpm for 5min, pour off the supernatant, repeat 8 times until the supernatant becomes dark green;

[0048] 4) Sonicate for 1h, centrifuge at 3500rpm for 1h, ...

Embodiment 2

[0066] A microfluidic paper-based sensor for detecting cardiac troponin I with MXene-enhanced signal based on electrochemical analysis, including the following steps:

[0067] a. Formation of plasma treated paper

[0068] 1) Design a paper-based model of a certain size, and form a hydrophilic area and a hydrophobic area through wax spray printing technology;

[0069] 2) Baking at 100°C for 5 minutes;

[0070] 3) Treat the paper with a plasma cleaner for 4 minutes to obtain the required paper;

[0071] 4) The plasma-treated paper needs to be sealed before adding samples to prevent oxidation.

[0072] b. Preparation of monolayer MXene

[0073] 1) Stir and mix 5mL water, 15mL hydrochloric acid and 1g lithium fluoride evenly;

[0074] 2) Add 1g of MXene precursor (MAX) and stir at 35°C for 24h;

[0075] 3) Centrifuge at 3500rpm for 5min, pour off the supernatant, repeat 8 times, wait until the supernatant

[0076] become dark green;

[0077] 4) Shake for 1h, centrifuge at 3...

Embodiment 3

[0095] The present invention will be further described below in conjunction with accompanying drawing.

[0096] figure 1 It is the detection principle of the microfluidic paper-based sensor for detecting cardiac troponin I based on the MXene enhanced signal for electrochemical analysis of the present invention.

[0097] figure 2 is the atomic force microscope (AFM) image of MXene

[0098] image 3 It is the impedance spectrogram characterization of antigen-antibody binding to the working electrode.

[0099] Figure 4 are the chronoamperometric spectra of different concentrations of cTnI.

[0100] Figure 5 is the specific detection map of the paper-based detection device

[0101] A microfluidic paper-based sensor for detecting cardiac troponin I with MXene-enhanced signal based on electrochemical analysis, including the following steps:

[0102] a. Formation of plasma treated paper

[0103] 1) Design a paper-based model of a certain size, and form a hydrophilic area ...

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Abstract

The invention discloses a paper-based electrochemical analysis device and method based on single-layer Mxene modification, and belongs to the technical field of biological nanotechnology. The sensor comprises a detection layer A and a sample adding layer B. The detection layer A is used for detecting cardiac troponin cTnI. The manufacturing method comprises the following steps: firstly, spraying wax and printing paraffin on whatman paper, and performing baking for a certain period of time to form a hydrophilic region and a hydrophobic region; secondly, performing silk-screen printing of a carbon working electrode on the detection layer A, and performing silk-screen printing of a carbon counter electrode and a silver-silver chloride reference electrode on the sample adding layer B; adding the MXene to the working electrode of the detection layer A again, then adding the cTnI antibody to the working electrode of the detection layer A, and adding the sample to the layer B for folding. Andfinally, adding a detection sample into the sample adding layer B, performing determining by utilizing immunoassay, and finally quantitatively detecting cTnI in the sample through an electrochemicalsignal.

Description

technical field [0001] The invention relates to biomaterials and nanomaterials, and belongs to the field of bionano technology, in particular to a microfluidic paper-based sensor for detecting cardiac troponin I based on single-layer MXene enhanced signals for electrochemical analysis. Background technique [0002] In 2007, the Whitesides team at Harvard University put forward new ideas on paper detection technology. Based on photolithography technology, they deposited hydrophobic polymers on the surface of the paper substrate, so that the paper substrate had the same hydrophobic / hydrophilic structure as the preset pattern. The hydrophobic part acts as a barrier to limit the reagent and liquid capillary flow range, and the hydrophilic part acts as a reagent reaction area or a liquid flow channel. Whitesides' idea provides a new approach to fluid handling and fluid analysis for applications in areas such as health diagnostics, environmental monitoring or food safety. The mo...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N27/327G01N27/30G01N27/416B01L3/00
CPCG01N33/6887G01N33/54373G01N33/5438G01N27/3278G01N27/308G01N27/416B01L3/5027
Inventor 于海东韩雨锋李林张承武刘志鹏刘金华黄维韩尧杰张晓盼杜超徐佳
Owner NANJING UNIV OF TECH
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