Microfluidic paper-based sensor for detecting cardiac troponin I based on single-layer MXene enhanced signal for electrochemical analysis
A technique for cardiac troponin and electrochemical analysis, applied in the field of biological nanometers, can solve the problems of difficult promotion, high cost, complicated sample processing, etc., and achieve the effects of wide application range, low cost and high sensitivity
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Embodiment 1
[0038] A microfluidic paper-based sensor for detecting cardiac troponin I with MXene-enhanced signal based on electrochemical analysis, including the following steps:
[0039] a. Formation of plasma treated paper
[0040] 1) Design a paper-based model of a certain size, and form a hydrophilic area and a hydrophobic area through wax spray printing technology;
[0041] 2) Bake at 90°C for 5 minutes;
[0042] 3) Treat the paper with a plasma cleaner for 4 minutes to obtain the required paper;
[0043] 4) The plasma-treated paper needs to be sealed before adding samples to prevent oxidation.
[0044] b. Preparation of monolayer MXene
[0045] 1) Stir and mix 5mL water, 15mL hydrochloric acid and 1g lithium fluoride evenly;
[0046] 2) Add 1g of MXene precursor (MAX) and stir at 35°C for 24h;
[0047] 3) Centrifuge at 3500rpm for 5min, pour off the supernatant, repeat 8 times until the supernatant becomes dark green;
[0048] 4) Sonicate for 1h, centrifuge at 3500rpm for 1h, ...
Embodiment 2
[0066] A microfluidic paper-based sensor for detecting cardiac troponin I with MXene-enhanced signal based on electrochemical analysis, including the following steps:
[0067] a. Formation of plasma treated paper
[0068] 1) Design a paper-based model of a certain size, and form a hydrophilic area and a hydrophobic area through wax spray printing technology;
[0069] 2) Baking at 100°C for 5 minutes;
[0070] 3) Treat the paper with a plasma cleaner for 4 minutes to obtain the required paper;
[0071] 4) The plasma-treated paper needs to be sealed before adding samples to prevent oxidation.
[0072] b. Preparation of monolayer MXene
[0073] 1) Stir and mix 5mL water, 15mL hydrochloric acid and 1g lithium fluoride evenly;
[0074] 2) Add 1g of MXene precursor (MAX) and stir at 35°C for 24h;
[0075] 3) Centrifuge at 3500rpm for 5min, pour off the supernatant, repeat 8 times, wait until the supernatant
[0076] become dark green;
[0077] 4) Shake for 1h, centrifuge at 3...
Embodiment 3
[0095] The present invention will be further described below in conjunction with accompanying drawing.
[0096] figure 1 It is the detection principle of the microfluidic paper-based sensor for detecting cardiac troponin I based on the MXene enhanced signal for electrochemical analysis of the present invention.
[0097] figure 2 is the atomic force microscope (AFM) image of MXene
[0098] image 3 It is the impedance spectrogram characterization of antigen-antibody binding to the working electrode.
[0099] Figure 4 are the chronoamperometric spectra of different concentrations of cTnI.
[0100] Figure 5 is the specific detection map of the paper-based detection device
[0101] A microfluidic paper-based sensor for detecting cardiac troponin I with MXene-enhanced signal based on electrochemical analysis, including the following steps:
[0102] a. Formation of plasma treated paper
[0103] 1) Design a paper-based model of a certain size, and form a hydrophilic area ...
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