Microfluidic paper-based sensor for detecting cardiac troponin I based on single-layer MXene enhanced signal for electrochemical analysis

Abstract

The invention discloses a paper-based electrochemical analysis device and method based on single-layer Mxene modification, and belongs to the technical field of biological nanotechnology. The sensor comprises a detection layer A and a sample adding layer B. The detection layer A is used for detecting cardiac troponin cTnI. The manufacturing method comprises the following steps: firstly, spraying wax and printing paraffin on whatman paper, and performing baking for a certain period of time to form a hydrophilic region and a hydrophobic region; secondly, performing silk-screen printing of a carbon working electrode on the detection layer A, and performing silk-screen printing of a carbon counter electrode and a silver-silver chloride reference electrode on the sample adding layer B; adding the MXene to the working electrode of the detection layer A again, then adding the cTnI antibody to the working electrode of the detection layer A, and adding the sample to the layer B for folding. Andfinally, adding a detection sample into the sample adding layer B, performing determining by utilizing immunoassay, and finally quantitatively detecting cTnI in the sample through an electrochemicalsignal.

Description

technical field

[0001] The invention relates to biomaterials and nanomaterials, and belongs to the field of bionano technology, in particular to a microfluidic paper-based sensor for detecting cardiac troponin I based on single-layer MXene enhanced signals for electrochemical analysis. Background technique

[0002] In 2007, the Whitesides team at Harvard University put forward new ideas on paper detection technology. Based on photolithography technology, they deposited hydrophobic polymers on the surface of the paper substrate, so that the paper substrate had the same hydrophobic / hydrophilic structure as the preset pattern. The hydrophobic part acts as a barrier to limit the reagent and liquid capillary flow range, and the hydrophilic part acts as a reagent reaction area or a liquid flow channel. Whitesides' idea provides a new approach to fluid handling and fluid analysis for applications in areas such as health diagnostics, environmental monitoring or food safety. The mo...

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