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Kit for detecting coxsackie virus A16

A coxsackie virus, A16 technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problem of lack of simple, fast and easy-to-use detection methods, and achieve high sensitivity, simple operation, and improved sensitivity. Effect

Inactive Publication Date: 2011-10-26
天津朝海科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of simple, rapid, easy-to-use and low-cost detection methods for Coxsackievirus A16

Method used

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  • Kit for detecting coxsackie virus A16
  • Kit for detecting coxsackie virus A16

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Effect test

Embodiment Construction

[0020] The kit of the present invention includes 2 tubes of solutions, one tube is 50 μl of nano-gold particle solution labeled with a molecular probe that can recognize the specific sequence of Coxsackievirus A16 type, and the other tube is 250 μl of a solution that can be amplified in the sample to be detected Coxsackievirus A16 DNA or a loop-mediated isothermal nucleic acid amplification system that amplifies Coxsackievirus A16 RNA in the sample to be detected after a reverse transcription process.

[0021] The gold nanoparticle solution contains gold nanoparticles with a diameter of 20 nm at a concentration of 1.2 nM, and the surface of the gold nanoparticles is marked with thiol-modified DNA probes.

[0022] The components of the loop-mediated isothermal nucleic acid amplification system are as follows: 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.4 M betaine (Sigma–Aldrich, St. Louis, MO), 10 mM MgSO4, 1.4 mM dNTPs, 1× ThermoPol reaction buffer (New England Bi...

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Abstract

The invention relates to a kit for detecting product deoxyribonucleic acid (DNA) which is amplified from coxsackie virus A16 subjected to a loop-mediated isothermal amplification technology by using nano-gold particles, belonging to the technical field of biological detection. By combining the loop-mediated isothermal amplification technology and biological nanometer technology, the sensitivity of biological molecular detection is improved, so the invention is a method with simplicity in operation, quickness and accuracy without professional instrument and equipment, and the kit can be widely applied to high sensitivity coxsackie virus A16 detection in the fields of household diagnosis, clinical diagnosis, infectious disease control, environmental monitoring, inspection and quarantine, biological technology and the like. The kit comprises two parts: (1) nano-gold particles marked with molecular probes which can identify the specific sequence of the coxsackie virus A16; and (2) a loop-mediated isothermal amplification system which can amplify the DNA of the coxsackie virus A16 in a sample to be detected, or amplify ribonucleic acid (RNA) of the coxsackie virus A16 in the sample to be detected after a reverse transcription process.

Description

technical field [0001] The invention relates to a kit for detecting Coxsackie virus A16 type DNA or RNA amplified by a loop-mediated isothermal nucleic acid amplification technology by using nano gold particles, and belongs to the technical field of biological detection. Background technique [0002] The high sensitivity, high specificity, simple and rapid detection of genes (DNA) and their transcription products (RNA) is very useful for the early diagnosis and treatment of diseases, as well as in the fields of health and epidemic prevention, environmental monitoring, inspection and quarantine, etc. Significance. In the past, more accurate and sensitive detection methods in this regard include fluorescent labeling, radioactive labeling and other methods. These methods require complex operations and special equipment, and their application range is very narrow. In 1993, Dr. Kary B. Mullis of PE Company invented the polymerase chain reaction (Polymerase Chain Reaction), refer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 孙国明
Owner 天津朝海科技有限公司
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