Positive control composition for reverse transcription polymerase chain reaction kit and preparation method thereof

Abstract

The invention provides a positive control composition for a reverse transcription polymerase chain reaction kit and a preparation method thereof, and the positive control composition is characterized in that a non-target virus is taken as positive control, a pair of primers are simultaneously designed for amplification, and the length of an amplified fragment is the same with that of a target gene of the reverse transcription polymerase chain reaction kit; and the non-target virus is inactivated by guanidine isothiocyanate. The positive control prepared by the method is safe, thereby avoiding virus expansion caused by direct use of a virus infection material in the prior art, playing a control role during an RNA (ribonucleic acid) extraction process and an RNA reverse transcription process when serving as the reverse transcription positive control of the RNA virus, proving that an RNA extraction reagent and substances used in a reaction system are normal in activity, facilitating the positive control when serving as the commercial kit and having very high practical value.

Description

technical field [0001] The invention belongs to the field of virus detection, relates to a positive control for reverse transcription polymerase chain reaction kit, more specifically relates to a positive control composition for reverse transcription polymerase chain reaction kit and its preparation method. Background technique [0002] The role of the control sample of the reverse transcription polymerase chain reaction kit in the experiment is: in each RT-PCR detection, the set up negative control sample and positive control sample are indispensable, when the negative control sample is detected as negative , indicating that the reagents in the whole process of the test are not contaminated; when the positive control sample is tested positive, it indicates that the working systems of RNA extraction, RNA reverse transcription, DNA amplification and electrophoresis identification are normal. Only when the test results of the negative control sample and the positive control s...

AI Technical Summary

Problems solved by technology

For the positive control of reverse transcription polymerase chain reaction kits for some highly pathogenic pathogenic microorganisms, most of these kits currently use the method of cloning the target fragment into the vector, which has the disadvantage that it cannot be used as Transcription process is controlled and prone to contamination

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