Preparation method and application of perfume for commelina communis cigarette
A technology for tobacco flavors and chickpeas, which is applied in the application, essential oil/spice, tobacco and other directions, can solve the problems of insufficient types of tobacco flavors and fragrances, insufficient preparation methods, etc., and achieves the safety and environmental protection of flavors and fragrances and processing technology. The effect of enriching the cigarette aroma and wide-ranging sources
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Embodiment 1
[0036] 1. Isolation, cultivation and identification of tobacco yellow soil-derived bacteria X7X
[0037] 1.1 Isolation of tobacco yellow soil-derived bacteria X7X
[0038] Soil samples were taken from the tobacco rhizosphere of the tobacco production base in Kunming, Yunnan Province, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 100mL of sterile water, shake and incubate at 30°C, 200rpm for 30min; dilute the concentration to 10 with sterile water -3 、10 -4 W / v times, take 0.1mL of the two concentrations of bacteria solution and evenly spread it on the R2A agar plate, set up three parallel experiments for each concentration of the bacteria solution, and cultivate it in a constant temperature incubator at 30°C for 2 weeks. Pick single colonies of different shapes from above, continue to use plate streaking method to isolate and purify the strains, inoculate them on R2A slant medium after numbering, or prepare glycerol suspens...
Embodiment 2
[0057] 1. Cultivation of Tobacco Yellow Soil Bacteria X7X
[0058] (1) Test tube slant culture
[0059] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate tobacco yellow soil-derived bacteria X7X, and cultivate it at 30°C for 2 weeks to obtain the test tube strain;
[0060] (2) Seed cultivation
[0061] The seed medium is R2A liquid medium, and the formula is: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; hydrogen phosphate Dipotassium 0.3g; magnesium sulfate 0.05g; distilled water 1000mL, pH7.2. Sterilize the medium at 121°...
Embodiment 3
[0079] 1. The cultivation method of tobacco yellow soil-derived bacterium X7X is the same as embodiment 2.
[0080] 2. Fermentation of chicken tongue powder
[0081] Grind the dried Cottonia baicalensis into a powder with a particle size of 80 meshes, weigh 1000g of Colicia baicalensis powder, balance the moisture to 12% in advance, spray 200mL of tobacco yellow soil-derived bacteria X7X inoculum, mix well, and mix the processed baicalin Put the chicken grass powder into a 22° C., 60% constant temperature and humidity box to ferment for 36 hours to obtain the fermented chicken grass powder.
[0082] 3. Preparation of Concentrated Extract of Cottonia qinensis
[0083] Put the fermented Digotis baicalensis powder in a sterilizing pot at 121°C for 40 minutes, then add 95% ethanol which is 5 times the mass of the fermented Diquitica baicalensis powder, heat and reflux for extraction at 65°C for 4 hours; the resulting heat reflux extraction The solution was suction filtered while...
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