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Operation method for influence of long-term coal pile on soil bacteria based on multi-dimensional indexes

An operation method and soil technology, applied in the field of soil microbial ecology, can solve the problems of toxicity, microbial acidification, etc., and achieve the effect of simple operation and high feasibility

Pending Publication Date: 2020-01-21
ELECTRIC POWER RES INST OF STATE GRID ANHUI ELECTRIC POWER +1
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Problems solved by technology

In addition, the soil may be acidifying or toxic to microorganisms due to the release of sulfur and heavy metal elements

Method used

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  • Operation method for influence of long-term coal pile on soil bacteria based on multi-dimensional indexes
  • Operation method for influence of long-term coal pile on soil bacteria based on multi-dimensional indexes
  • Operation method for influence of long-term coal pile on soil bacteria based on multi-dimensional indexes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0068]Four power plants in a city (TJ power plant: 10 years, PW power plant: 20 years, LH power plant: 30 years, TJA power plant: 70 years) were selected as the research objects, and the effects of long-term coal piles on soil were analyzed based on soil physical and chemical properties and organic matter indicators. Effects of organic matter and soil bacterial diversity.

[0069] (1) Collect soil samples

[0070] Soil samples were collected from four power plants, each containing two sampling sites, one in the coal yard and the other in the lawn soil as a control. At each sampling point, three 6 m × 6 m sampling points were divided, and five organic layer soil samples were randomly collected and pooled from the soil core 0–10 cm depth below the coal seam or turf layer at each sampling point, for a total of 24 samples. Fresh soil samples were passed through a 2 mm sieve to remove visible roots and other residues. Each of the 24 samples was divided into two sub-samples, one ...

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Abstract

The invention discloses an operation method for influence of long-term coal pile on soil bacteria based on multi-dimensional indexes, which comprises the following steps: (1) selecting research areasand collecting soil samples; (2) analysis of soil physical and chemical properties; (3) DNA extraction, PCR amplification and Illumina MiSeq sequencing; (4) processing of 16S and 18S sequencing data;(5) analysis of the influence of long-term coal piling on soil properties and microbial communities. Through the above steps, the influence of long-term coal piling in power plants on soil organic matter and soil bacterial diversity can be analyzed, which is of great significance for guiding environmental impact assessment and soil vegetation restoration of coal storage yards in coal-fired power plants.

Description

technical field [0001] The invention relates to the field of soil microbial ecology, in particular to an operation method aimed at the influence of long-term coal piles on soil bacteria based on multidimensional indicators. Background technique [0002] China is the world's largest coal producer and consumer. In China, coal accounts for about 74% of its annual energy consumption, and it is expected that coal as the main energy source may further increase in the near future. The development and utilization of coal resources will inevitably bring about a series of ecological and environmental problems, such as land destruction, vegetation reduction and biodiversity reduction. Particularly in coal mining areas, previous studies have provided evidence of ecosystem degradation due to coal mining, manifested by reductions in plant and microbial diversity and changes in soil structure and properties. After mining, large quantities of coal are transported to the coal yard of the p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/04
CPCC12Q1/6869C12Q2531/113C12Q2535/122C12Q2537/165
Inventor 马大卫陈中元王贻平庄媛张本耀何军葛源黄齐顺申聪聪凌默侬张其良朱帅程靖
Owner ELECTRIC POWER RES INST OF STATE GRID ANHUI ELECTRIC POWER
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