Method for preparing biocontrol bacterial fertilizer and eliminating continuous cropping obstacle of muskmelon by using biocontrol bacteria

A technology of bio-control bacteria and bio-control bacteria, which is applied in the field of using bio-control bacteria to prepare bio-control bacterial fertilizer and eliminate obstacles to continuous cropping of melons and melon planting. To achieve the stability and safety of bacterial fertilizer products, inhibit the growth of soil fungi, and promote the growth of soil bacteria

Inactive Publication Date: 2019-06-04
曹翠玲
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Problems solved by technology

[0005] Although soil-borne diseases can eliminate soil fungi through root irrigation and other measures, when pesticides are used to kill soil fungi, they also kill other microorganisms in the soil; long-term production of a single crop variety will eventually lead to ch

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  • Method for preparing biocontrol bacterial fertilizer and eliminating continuous cropping obstacle of muskmelon by using biocontrol bacteria
  • Method for preparing biocontrol bacterial fertilizer and eliminating continuous cropping obstacle of muskmelon by using biocontrol bacteria
  • Method for preparing biocontrol bacterial fertilizer and eliminating continuous cropping obstacle of muskmelon by using biocontrol bacteria

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Embodiment Construction

[0034] The specific implementation manner of the present invention will be described below in conjunction with the accompanying drawings and examples.

[0035] The present invention is a method for preparing bio-control bacterial fertilizer and eliminating the obstacle of continuous cropping of melons by using bio-control bacteria, and the process includes:

[0036] 1. Strain isolation, identification and cultivation

[0037] 1.1 Screening of high temperature resistant strains

[0038] After 15 parts of rhizosphere soil collected from healthy kiwi fruit orchards in Meixian County, Shaanxi Province were treated for 20 minutes at 90 times, 2g was weighed, put into a 50mL conical flask filled with 18mL sterile water, and oscillated at 180r / min at room temperature 30 minutes is the bacterial suspension diluted 10 times. The above bacterial suspension was diluted 10 times again and spread evenly on LB solid medium. Observing the morphological characteristics of a single colony a...

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Abstract

A method for preparing a biocontrol bacterial fertilizer by using biocontrol bacteria comprises the following steps: using bacillus subtilis obtained by separation in a kiwi fruit garden as a strain,and separating and identifying the strain; adding sterilized soybean meal, ammonium nitrate, cane sugar, magnesium sulfate and manganese sulfate into a sterilized carrier matrix, adding sterile waterand a seed liquid of the biocontrol bacteria, fully and uniformly stirring to obtain a fermentation raw material; fermenting the fermentation raw material to guarantee that the biocontrol bacteria count of the matrix carrier reaches 1010 CFU/g DW carrier matrix, and uniformly spraying the prepared biocontrol bacterial fertilizer into a muskmelon field according to 135 kg/mu one month before transplantation of muskmelon seedlings. Thereby, soil fungi growth can be inhibited, soil microflora is balanced, and the content of the main ingredient cumaric acid of muskmelon root exudates in soil is reduced. And then, continuous cropping obstacle in muskmelon production is eliminated, agricultural sustainable development is realized, and the ecological environment is maintained.

Description

technical field [0001] The invention belongs to the technical field of planting, relates to the planting of melons, in particular to a method for preparing bio-control bacterial fertilizer and eliminating obstacles to continuous cropping of melons by using bio-control bacteria. Background technique [0002] Continuous cropping obstacle refers to the phenomenon that after planting the same species or the same family of crops in the same plot for many years, even if normal cultivation and management measures are adopted, the plant growth will be weakened, the pests and diseases will be aggravated, and the yield and quality will decline. In ancient my country, there were some records about the aggravation of crop diseases after continuous cropping. It was called continuous stubble disease, and European and American countries called it soil-borne disease, continuous cropping obstacle, and replanting disease. Japan calls it the taboo phenomenon. Continuous cropping obstacles are...

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Application Information

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IPC IPC(8): C05F17/00C05G3/00C05G3/04A01C21/00C05G3/80
CPCY02W30/40
Inventor 曹翠玲谢惠琴
Owner 曹翠玲
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