Application of Agromyces sp. MT‑E in Degradation of Various Phthalates

A technology of dibutyl phthalate and diisooctyl phthalate, which is applied in the field of soil fungus MT-E in degrading various phthalates, can solve the problem of degradable phthalates that have not yet been seen. Phthalates and other problems, to achieve the effect of enriching the germplasm resource bank, easy to cultivate, and strong adaptability

Active Publication Date: 2017-10-31
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil mold ( Agromyces sp.) is a genus widely present in the soil. It has been reported that some strains of this genus can efficiently degrade phenol, pesticide thiamethoxam and resistance to heavy metals, but it has not been found that it can degrade phthalo formate reports

Method used

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  • Application of Agromyces sp. MT‑E in Degradation of Various Phthalates
  • Application of Agromyces sp. MT‑E in Degradation of Various Phthalates
  • Application of Agromyces sp. MT‑E in Degradation of Various Phthalates

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Isolation and identification of strains

[0030] Collect farmland soil that has been used in plastic greenhouses all the year round, weigh 5 g of fresh soil sample into a 150 mL Erlenmeyer flask containing 50 mL sterile water, incubate at 30°C, 150 rpm for 3 days, and add 5 mL of sludge suspension to 100 mL DBP and DEHP (50 mg / L respectively) in MSM medium. After culturing at 30°C and 150 rpm for 7 days, the inoculum was continuously enriched and transferred 10 times at a volume of 1 mL each time, and the content of PAEs in the medium was increased to 1000 mg / L accordingly. Then dilute the culture solution 10 times 3 ~10 5 Spread on the LB solid plate and incubate at 30°C for 1 to 3 days. After a single bacteria grows on the plate, a single colony is picked and streaked for purification several times, and a bacteria strain is isolated, numbered MT-E. Then the strain was inoculated on the LB solid plate and cultured upside down at 30°C for 7 days to observe the...

Embodiment 2

[0038] Example 2 Soil mold ( Agromyces sp.) Degradation effect of strain MT-E on DBP and DEHP

[0039] S1. Preparation of bacterial suspension: The purified MT-E strain was connected to 10 mL of LB liquid medium for overnight activation and culture to the logarithmic phase. The bacteria were collected by centrifugation at 5000 rpm for 10 min, and the bacteria were washed 3 times with PBS Resuspend afterwards, adjust OD 600 nm =0.8 as a bacterial suspension.

[0040] S2. Inoculate 1 mL of the above bacterial suspension into 100 mL MSM culture medium containing 200 mg / L DBP and DEHP (each containing 200 mg / L), use non-inoculation as a negative control, and adjust the pH to 7.5. Set of three repetitions. Incubate at 30°C and 150 rpm in a constant temperature shaker for 7 days. Take samples and extract samples on 0, 1, 3, 5, and 7 days respectively. Determine the degradation of DBP and DEHP in the samples by GC / MS, and use a spectrophotometer. Measure the bacterial growth OD at ea...

Embodiment 3

[0045] Example 3 Remediation effect of strain MT-E on soil contaminated by DBP and DEHP

[0046] S1. Preparation of test soil: The soil is the paddy soil of the farm of South China Agricultural University. After air-drying, it is passed through a 60-mesh sieve, and the pH is 6.7. DBP and DEHP are added to the soil so that the content in the soil reaches 100 mg / kg, and double Distilled water is adjusted to the field water holding capacity (about 30%) and aged for 15 days in the dark.

[0047] S2. Weigh the above-mentioned 100 mg kg -1 Put 200 g of DBP and DEHP soil in a triangular flask, add 50 mL of bacterial suspension to the soil and mix well. In addition, the treatment without inoculation was used as the control group. Adjust the soil to the field water holding capacity (about 30% of the water content), cultivate in a 30°C incubator protected from light, and sample and extract regularly at 0, 2, 4, 6, 8, and 10 days, and then use GC / MS Determine the residual amount of DBP and ...

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Abstract

The invention specifically discloses application of Agromyces sp. MT-E in degradation of a variety of phthalates, which belongs to the technical field of biological treatment of environmental pollutants. The Agromyces sp. MT-E is preserved in China Center for Type Culture Collection (CCTCC) on January 22, 2015, with an accession number of CCTCC M2015054. The Agromyces sp. MT-E can use DBP and DEHP as sole carbon source for aerobic degradation, and degradation rates reach 97.06% and 86.25% respectively when the Agromyces sp. MT-E is cultured in inorganic salt mediums respectively including DBP and DEHP with a concentration of 200 mg / L as the sole carbon source for 7 d. After the Agromyces sp. MT-E is inoculated into contaminated soil for 10 d, the degradation rates of DBP and DEHP in the soil reach 77.29% and 55.51%, respectively; and such high-efficiency degradation rates prove that the Agromyces sp. MT-E has good application prospect in restoration of soil contaminated by PAEs.

Description

Technical field [0001] The present invention relates to the technical field of biological treatment of environmental pollutants, in particular, to soil mold ( Agromyces sp.) Application of MT-E in the degradation of various phthalates. Background technique [0002] Phthalates, also known as phthalates, abbreviated as PAEs, are a general term for esters formed by phthalic acid. Among them, dibutyl phthalate (di- n -butylphthalate, DBP) and di-(2-ethylhexyl)phthalate (DEHP) are the two most widely used plastic plasticizers, and their content in PVC plastics can reach 40~60% . In recent decades, the use of plastic greenhouses and agricultural films in my country has increased dramatically. With the extensive use of plastic films, more and more DBP and DEHP are released into farmland soil. They have become the most frequently detected organic pollutants in farmland soil, with the detection level reaching mg / kg. Studies have shown that these two phthalates have high toxicity, terat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10C02F3/34B01D53/84B01D53/72C12R1/645C02F101/34
CPCY02A50/20
Inventor 莫测辉赵海明李彦文蔡全英李慧杜欢向垒陈学斌
Owner JINAN UNIVERSITY
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