31results about How to "Solve industrialization problems" patented technology

The invention discloses a sulfur-substituted podophyllum derivative and a bioconversion, separation and purification method thereof. The sulfur-substituted podophyllum derivative with antitumor activity, shown as Formula (I) or (II), is obtained by modifying the molecular structure of podophyllotoxin or 4'-demethylepipodophyllotoxin through a bioconversion method. Preliminary study on in-vitro tumor cytotoxicity shows that the sulfur-substituted podophyllum derivative disclosed by the invention has stronger inhabitation action on the growth of tumor cells such as BGC823, HepG2, A549 and the like, and thus can be used as an antitumor compound to achieve a potential drug development value. According to the invention, the podophyllotoxin and the 4'-demethylepipodophyllotoxin are converted into the sulfur-substituted podophyllum derivative by using a bioconversion method, and therefore, the sulfur-substituted podophyllum derivative has the advantages of low cost and E-factor, simplicity in operation, easiness for culturing, controllable quality, capability of effectively preventing chemical residuals and the like.

The invention relates to ganoderma lucidum refined polysaccharides with significant assistant antitumor activity and a preparation method and application of the ganoderma lucidum refined polysaccharides. The refined polysaccharides is heteropolysaccharide which is mainly prepared from mannose, glucosamine, glucuronic acid, glucose, galactose, xylose and fucose in a molar ratio of (3 to 4) to 1 to(1.3 to 1.7) to (56 to 65) to (4 to 6) to (1.3 to 1.8) to (2.5 to 3.5), the absolute purity is greater than 52%, the weight average molecular weight is 7500-8500D, and the molecular weight distribution index is 1.3 to 1.5. The preparation method comprises the following steps of dynamically extracting by hot water, coarsely filtering to remove residues, carrying out fine filtration and clarifying,deproteinizing and decoloring by in-series resin beds, concentrating and removing impurities by an ultrafiltration membrane, and carrying out spray-drying or alcohol precipitation air drying. The method is intelligent and energy-saving, can solve industrial difficult problems, and has significant large scale advantage and good product safety, effectiveness, controllability and stability. The polysaccharides have significant functional activity in assisting in antitumor and are useful for the development of drugs for prevention and treatment of tumors and the development of food and health products in related fields.

The invention relates to ganoderma spore powder refined polysaccharide with significant auxiliary anti-tumor activity and a preparation method and application thereof, and belongs to the field of medicinal-fungus extraction and separation and the field of drug and food application development. The refined polysaccharide is mainly prepared from heteropolysaccharide composed of mannose, glucose, galactose and fucose according to the molar ratio of 1: (40-50): (4-5.5): (1.5-2), the purity of the refined polysaccharide is greater than 80%, and the weight-average molecular weight is 160-200 KD. Thepreparation method of the ganoderma spore powder refined polysaccharide includes the steps of using hot water for dynamic extraction, removing residues by combining sedimentation and rough filtration, conducting refined filtration and clarification, removing impurities through ultrafiltration-membrane concentration, and drying through spray or airing through alcohol precipitation. The preparationmethod of the ganoderma spore powder refined polysaccharide is environmentally friendly and smart, saves energy, can solve industrialized difficulties and has significant scale advantages, prepared products meet the requirements, about the safety, availability, controllability and stability, in a drug and medicine quality controlling system. The ganoderma spore powder refined polysaccharide has significant functional activity at the auxiliary anti-tumor aspect and can be used for developing tumor prevention and treating drugs, and development of food and healthcare products in related fieldscan be carried out.

The invention discloses a method for preparing alpha-androstanol, comprising the following steps: liquid fermentation is carried out on tuber bacterial strain to obtain zymotic fluid or mycelium containing the alpha-androstanol. The invention also discloses a method for detecting content of the alpha-androstanol in the zymotic fluid or the mycelium. In the invention, tuber liquid fermentation is adopted to produce alpha-androstanol, compared with chemosynthesis, the method of the invention has the advantages of low cost, short cycle, easy operation controllable quality and capability of preventing chemical residue, etc.

The invention discloses a multistage sweeping type silicon slice texturing and processing device and a control method thereof. The multistage sweeping type silicon slice texturing and processing device comprises a reaction chamber. A silicon slice moving carrier is arranged in the reaction chamber. At least two plasma downstream sweeping type processing units are arranged on the reaction chamber. A front buffer chamber and a front sluice valve are arranged at an inlet of the reaction chamber. A rear buffer chamber and a rear sluice valve are arranged at an outlet of the reaction chamber. A reaction chamber vacuum suction port is arranged at a position, close to the rear buffer chamber, on the reaction chamber. A constant-temperature radiation block is arranged below the silicon slice moving carrier. The multistage sweeping type silicon slice texturing and the processing device and a control method thereof have the advantages that the multistage sweeping type silicon slice processing units are adopted, independent working gas flow proportion and etching time can be adopted conveniently, and a rapid and efficient silicon slice processing process is achieved; etching speed is increased by the silicon slice moving carrier and the constant-temperature radiation block, so that online continuous processing is achieved, and silicon slice texturing effect is improved.

The invention relates to a method for preparing nonapeptide microspheres without sensitized auxiliary materials. The method comprises the following steps: pretreating a raw material leuprolide acetate; dissolving the treated leuprolide acetate into water to serve as an inner water phase solution, adding a lactic-glycolic acid polymer into dichloromethane to serve as an oil phase solution; mixing the prepared two-phase solution, and regulating the ultrasonic power, so that the mixed solution becomes milk white, pouring the mixed solution into polyvinyl alcohol for stirring at a high speed, transferring the stirred solution into evaporation equipment, so that the dichloromethane volatilizes out; centrifugally screening, washing and collecting semi-finished nonapeptide microspheres, adding a certain amount of mannitol into the washed semi-finished microspheres, starting prefreezing at the temperature of 35 DEG C below zero, preserving the heat at 37 DEG C for 8 hours, ending, performing freeze-drying for 51 hours, thereby obtaining the freeze-dried powder, namely the product. According to the preparation method disclosed by the invention, the problem of immunoreaction of gelatin auxiliary materials in the microsphere preparation is solved by optimizing the process parameters, and the method is suitable for industrialized production.

The invention specifically discloses a milk replacer for squabs and a preparation method for the milk replacer, and solves the problem of restrictions of lactation of conventional 1 to 10-day aged squabs to squab intensification and industrialization. The milk replacer for the squabs is prepared from the following air-dried solid raw materials in parts by weight: 250 to 300 parts of egg powder, 150 to 200 parts of fish meal, 150 to 200 parts of soybean meal, 100 to 150 parts of whole milk powder, 80 to 100 parts of vegetable oil, 50 to 80 parts of corn gluten meal, 40 to 80 parts of lactic acid powder, 6 to 8 parts of calcium hydrogen phosphate, 2 to 3 parts of edible salt, 0.8 to 1 part of composite trace element, 0.6 to 1 part of composite vitamin and 0.1 to 0.2 part of an antioxidant. The prepared milk replacer is nutritionally balanced, high in palatability and easy to digest and absorb and accords with gastrointestinal digestion characteristics of the squabs, and has the advantages of convenience in storage and preparation and capability of meeting squab intensive-feeding production requirements, and requirements of 1 to 10-day aged squabs on nutrition are met.

The invention discloses a method for transforming podophyllotoxin into 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin, comprising the following steps of: (1) adding podophyllotoxin into a liquid fermentation medium, and carrying out biotransformation by inoculating second-class liquid seeds of humic soil fungi; (2) separating and purifying the product from the Step (1), followed by adding into the liquid fermentation medium, carrying out biotransformation by inoculating second-class liquid seeds of podophyllum taxa endophytes; then continuously carrying out biotransformation by adding ligustrazine, and collecting the transformation product. The invention also discloses a method for purifying 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin from the above product and a method for detecting the content of 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin. According to the invention, the molecular structure of podophyllotoxin is modified by the biotransformation method, which has advantages of mild reaction condition, low E-factor, quality controllability and simple separation process.

The invention discloses a pharmaceutical composition of palipeddone, belongs to medicine field, and relates to an antipsychotic pharmaceutical composition, wherein the pharmaceutical composition comprises palipeddone active component, controlled-release auxiliary material and other acceptable auxiliary material in pharmacy. The pharmaceutical composition provided by the invention solves the problem of difficult medicine taking by mental patients, and has advantages of simple technology, low cost and easy industrialization, and can better satisfy clinic needs.

The invention discloses a combined extraction method of different functional components in citrus fruit peel residues. The method comprises the steps: adding water into the citrus fruit peel residues, adjusting the pH value to be acidic, stirring and extracting at constant temperature, centrifuging extracted pectin liquid, collecting supernate, carrying out alcohol precipitation on the supernate, precipitating and drying to obtain pectin; adding water into the obtained residues, stirring, firstly, carrying out physical pretreatment, homogenizing, carrying out alcohol precipitation, precipitating and drying to obtain citrus fibers; and carrying out alcohol recovery on the alcohol precipitation waste liquid, and carrying out purification and concentration to obtain limonin and flavonoid substances. According to the invention, three functional products of pectin, limonin and flavonoid extracts and citrus fibers applied in different fields are obtained through a continuous step-by-step separation and extraction process and a combined chemical and physical modification process, and through reasonable arrangement and planning of an industrialization route, 'thorough eating and squeezing' is really realized. As a novel low-pollution agricultural product biological environment-friendly processing scheme, the combined extraction process route has good conversion application expectation.

The invention relates to a preparation method of a sulfydryl alkenyl ester compound, belongs to the field of organic chemical synthesis, and aims to solve the problems of high risk of large-scale production and the like, and the synthesis route provided by the invention comprises the following three steps: (1) carrying out a reaction on a starting material ethyl 2-oxo carboxylate compound and Tf2O (trifluoromethanesulfonic anhydride) under the action of alkali and a solvent A, so as to generate an enol intermediate 1 protected by trifluoromethanesulfonyl; (2) carrying out substitution reaction with a sulfo reagent to obtain an intermediate 2; and (3) performing deprotection on the intermediate 2 under the action of a deprotection reagent to obtain a target product 3. The method for preparing the sulfydryl alkenyl ester compound has the beneficial effects that the process is novel, toxic gases such as hydrogen sulfide are prevented from being used, the overall safety and environmental protection property of the process are improved, and the method is more suitable for large-scale production and solves the industrialization problem.

The invention discloses a culture medium for culturing lycopodium japonicum and an application of the culture medium. The culture medium is prepared from the following raw material ingredients in proportion: an MS culture medium, saccharose, carrageenan, mashed carrots, peptone, mashed bananas and activated charcoal which are in the ratio of 1L: (23g to 27g): (5g to 7g): (18g to 22g): (1.8g to 2.2g): (90g to 110g): (0.8g to 1.2g), wherein the MS culture medium per liter contains 1.8mg to 2.2mg of 6-BA, 0.8mg to 1.2mg of NAA and 0.15mg to 0.25mg of KT. According to the culture medium and the application thereof, by a specific ingredient proportioning method, the culture medium can be used for achieving the one-step mature-shoot rapid propagation of the lycopodium japonicum.

The invention discloses a preparation technology of a high-toughness zinc-based copper-titanium alloy wire, which comprises the following technological processes: smelting a zinc-based copper-titanium alloy by using a 2t resistance furnace, preparing the zinc-based copper-titanium alloy wire by adopting an extrusion deformation and drawing deformation technology, firstly, directly extruding a bar with the diameter of 100mm into a rod with the diameter of 8.4 mm, and then, carrying out seven passes of hot drawing to obtain the high-toughness zinc-based copper-titanium alloy wire. And the 8.4 mm rod material is deformed into a 3 mm wire material. The preparation method has the advantages that on one hand, the alloy wire with better toughness is prepared; and on the other hand, the problem that the zinc-based copper-titanium alloy wire is prone to being broken in the forming process is solved, and the industrialization problem of the zinc-based copper-titanium alloy wire is also solved.

The invention discloses a continuous casting and rolling preparation method of a novel zinc alloy wire. The continuous casting and rolling preparation method comprises the following steps: (1) smelting a Zn-Ce intermediate alloy; (2) zinc alloy smelting; (3) zinc alloy continuous casting; and (4) zinc alloy continuous rolling. Compared with the prior art, the method has the advantages that the zinc-copper-titanium alloy wire for thermal spraying is prepared by adopting a continuous casting and rolling technology, a Zn-Cu-Ti-Ce alloy is prepared in an induction furnace with the capacity of 2 t, a Zn-Cu-Ti-Ce alloy bar with the diameter of 50 mm is formed in a continuous casting machine, the bar is subjected to 14-pass rolling deformation, the alloy wire with the diameter of 3 mm is prepared, and the zinc-copper-titanium alloy wire for thermal spraying is obtained. And the problem of industrialization of the zinc-based copper-titanium alloy wire is solved.