Ganoderma spore powder refined polysaccharide with significant auxiliary anti-tumor activity and preparation method and application thereof

A technology of Ganoderma lucidum spore powder and anti-tumor activity, applied in anti-tumor drugs, organic active ingredients, medical preparations containing active ingredients, etc., can solve the problems of accelerated death cases, physical failure of patients, etc. The effect of index improvement and auxiliary anti-tumor activity

Active Publication Date: 2018-02-23
GUANGDONG YUEWEI EDIBLE FUNGI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current mainstream cancer treatment methods include cytotoxic drugs and radiation therapy, but these met...

Method used

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  • Ganoderma spore powder refined polysaccharide with significant auxiliary anti-tumor activity and preparation method and application thereof
  • Ganoderma spore powder refined polysaccharide with significant auxiliary anti-tumor activity and preparation method and application thereof
  • Ganoderma spore powder refined polysaccharide with significant auxiliary anti-tumor activity and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Use 40kg of the whole Ganoderma lucidum spore powder residual residue that has been extracted from the whole Ganoderma lucidum spore oil in Example 2 of the invention patent CN 100593410C for standby, add 600L of purified water to the multi-energy extraction tank, and after boiling with steam, slowly add the above-mentioned For the residue, continue to stir and maintain a slight boil for 1 hour, let it settle and cool to below 50°C, take out the supernatant, filter it with a plate and frame, repeat the extraction of the residue once according to the above method, and combine the crude filtrate. The crude filtrate used 500nm tubular ceramic microfiltration membrane (CX-30-1016-4-19 type, 4 parallel connections, membrane area 0.952m 2 ) for clarification, the operating temperature is 50°C, the operating pressure is 0.35Mpa, the membrane surface flow rate is 4.5m / s, and the permeate flux is 360LMH. Repeat again. The clarified liquid was concentrated with a PT4040C2016 org...

Embodiment 2

[0073] Use 30kg of the whole Ganoderma lucidum spore powder residue that has been extracted from the whole Ganoderma lucidum spore oil in Example 2 of the invention patent CN 100593410C for standby, add 700L of purified water to the multi-functional extraction tank, and after boiling with steam, start the feed liquid pump under countercurrent forced circulation Slowly add the above residue, start the feed liquid pump at intervals of 10 minutes to force circulation for 5 minutes, maintain a slight boil for 1 hour, let it settle and cool to below 50°C, take out the supernatant, and use a filter bag filter with a filtration accuracy of 0.5 μm for coarse filtration , the residue was extracted once more according to the above method, and the crude filtrate was combined. The crude filtrate is finely filtered with a PP microporous folded filter element with a filtration accuracy of 0.1 μm. The fine filtrate was concentrated with a PT4040C2016 organic ultrafiltration membrane produced...

Embodiment 3

[0076] Determination of Protein and Polysaccharide Contents in Refined Polysaccharides of Whole Ganoderma Lucidum Spore Powder by UV-Vis Spectrophotometry

[0077] Determination of protein content: BCA method was used for determination, BCA protein concentration determination kit (Enhanced) (EnhancedBCA Protein Assay Kit) was purchased from Shanghai Beyontian Biotechnology Co., Ltd., and the detection was done according to the detailed instructions.

[0078] Determination of polysaccharide content: Determination by anthrone-sulfuric acid method, according to the steps in the "Chinese Pharmacopoeia" 2015 edition under the item of Ganoderma lucidum [Content Determination] Polysaccharides".

[0079] The protein content and polysaccharide content in the refined polysaccharide of the whole ganoderma lucidum spore powder prepared in Example 1 were measured according to the above method respectively, and the protein content of the refined polysaccharide of the whole ganoderma lucidum ...

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Abstract

The invention relates to ganoderma spore powder refined polysaccharide with significant auxiliary anti-tumor activity and a preparation method and application thereof, and belongs to the field of medicinal-fungus extraction and separation and the field of drug and food application development. The refined polysaccharide is mainly prepared from heteropolysaccharide composed of mannose, glucose, galactose and fucose according to the molar ratio of 1: (40-50): (4-5.5): (1.5-2), the purity of the refined polysaccharide is greater than 80%, and the weight-average molecular weight is 160-200 KD. Thepreparation method of the ganoderma spore powder refined polysaccharide includes the steps of using hot water for dynamic extraction, removing residues by combining sedimentation and rough filtration, conducting refined filtration and clarification, removing impurities through ultrafiltration-membrane concentration, and drying through spray or airing through alcohol precipitation. The preparationmethod of the ganoderma spore powder refined polysaccharide is environmentally friendly and smart, saves energy, can solve industrialized difficulties and has significant scale advantages, prepared products meet the requirements, about the safety, availability, controllability and stability, in a drug and medicine quality controlling system. The ganoderma spore powder refined polysaccharide has significant functional activity at the auxiliary anti-tumor aspect and can be used for developing tumor prevention and treating drugs, and development of food and healthcare products in related fieldscan be carried out.

Description

technical field [0001] The invention relates to a refined polysaccharide of whole Ganoderma lucidum spore powder with significant auxiliary antitumor activity and its preparation method and application, belonging to the fields of extraction and separation technology of medicinal fungi and application and development of medicine and food. Background technique [0002] Ganoderma lucidum (scientific name: Ganoderma Lucidum Karst), which is umbrella-shaped in shape, with a kidney-shaped, semi-circular or nearly round cap, is the fruiting body of the fungus Ganoderma lucidum of Polyporaceae; it has the effects of nourishing qi and calming the nerves, relieving cough and asthma, and prolonging life; For dizziness and insomnia, palpitation, shortness of breath, neurasthenia, fatigue and cough. [0003] The growth process of Ganoderma lucidum includes: spore → mycelium → fruiting body → releasing spores. Ganoderma lucidum spores, mycelium and fruiting body are different stages of i...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCA61K31/715C08B37/0003C08B37/006
Inventor 帅欧陈地灵吴清平谢意珍杨柏华谭许朋
Owner GUANGDONG YUEWEI EDIBLE FUNGI TECH
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