Culture medium for culturing lycopodium japonicum and application of culture medium

A technology of culture medium and public stone pine, applied in the field of plant cultivation, can solve problems such as shortage of supply, and achieve the effect of solving industrialization problems

Active Publication Date: 2018-06-26
FUJIAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As an authentic Chinese herbal medicine species in Zhangzhou, male stone pine has no record of artificial cultivation. The market mainly sells local or foreign (country) wild male stone pine. Coupled with the enhancement of people's awareness of health care, it has gradually gained people's understanding and attention. The price of wild male stone pine is increasing year by year. From May to October every year, the market price of fresh products has surpassed that of golden clematis, reaching 150 yuan per catty, and the supply is still in short supply.

Method used

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  • Culture medium for culturing lycopodium japonicum and application of culture medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 culture medium

[0028] (1) Weigh each raw material component in proportion: MS medium: sucrose: carrageenan: carrot puree: peptone: banana puree: activated carbon=1L: 25g: 6g: 20g: 2g: 100g: 1.0g, wherein each liter of MS The medium contains 2mg of 6-BA, 1.0mg of NAA and 0.2mg of KT, and the preparation table of MS mother solution is as follows:

[0029]

[0030] (2) Add sucrose, carrageenan, carrot puree, peptone, banana puree and activated carbon into the MS medium, heat and stir evenly;

[0031] (3) Carry out high-pressure steam sterilization after subpackaging the material gained in step (2), then cool to solidification at room temperature;

[0032] (4) Place the material obtained in step (3) for 3 to 5 days in summer or 5 to 8 days in winter to remove the contaminated culture medium.

Embodiment 2

[0033] The cultivation of embodiment 2 male stone pine

[0034] (1) Put the explants or tissue-cultured aseptic seedlings of the red club stone pine obtained through cleaning and disinfection into the culture medium prepared in Example 1 to obtain 2 to 3 leaves and a height of 6 For bottle seedlings of ~7cm, the culture conditions are as follows: the growth temperature is 5 ~ 35°C (preferably 15 ~ 28°C), the light intensity is 1500lx ~ 4000lx (preferably 3500lx), and it is sterilized once every 15 ~ 30d (can be fumigated with formalin, Spray new gel or cooperate with ultraviolet light), wherein the specific illumination is: summer daytime illumination, winter nighttime illumination, the duration is 10-12h; this step is divided into two situations, when using naturally grown plants as explants It includes three steps of cleaning, disinfection and inoculation; when using tissue-cultured aseptic vaccines, perform aseptic inoculation directly, as follows:

[0035] a, natural grow...

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Abstract

The invention discloses a culture medium for culturing lycopodium japonicum and an application of the culture medium. The culture medium is prepared from the following raw material ingredients in proportion: an MS culture medium, saccharose, carrageenan, mashed carrots, peptone, mashed bananas and activated charcoal which are in the ratio of 1L: (23g to 27g): (5g to 7g): (18g to 22g): (1.8g to 2.2g): (90g to 110g): (0.8g to 1.2g), wherein the MS culture medium per liter contains 1.8mg to 2.2mg of 6-BA, 0.8mg to 1.2mg of NAA and 0.15mg to 0.25mg of KT. According to the culture medium and the application thereof, by a specific ingredient proportioning method, the culture medium can be used for achieving the one-step mature-shoot rapid propagation of the lycopodium japonicum.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a medium for cultivating male stone pine and its application. Background technique [0002] The male stone pine, orchidaceae, is a rare medicinal material, mainly distributed in the central mountainous area of ​​Zhangzhou City, Fujian Province. Because it grows in the rock crevices beside the deep mountain valleys, it is named "Gongshi Song". In Fujian The southern Fujian region is widely welcomed by consumers and has certain market development potential. At present, its price is about 10 to 20 times that of ordinary green grass. It is pungent in nature and bitter in taste, has diuretic and antipyretic effects, clears fire and dampness, and is often used in the folk to treat sores, and can also be used to treat prolapse of the anus. According to folk doctors: Gongshisong is a wild natural plant, cold in nature and sweet in taste, sweet in taste and clear in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 郑涛蔡坤秀王龙平潘腾飞林艺华
Owner FUJIAN INST OF TROPICAL CROPS
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