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Preparation, purification and content detection methods for cerebroside

A technology for separation and purification of cerebrosides, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of high cost, small scale, low output, etc., and achieve low cost and short cycle , quality controllable effect

Inactive Publication Date: 2014-03-05
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to overcome the disadvantages of small scale, low output and high cost in the prior art, and provide a new preparation method of cerebroside, which is practicable, simple to operate, short in cycle and high in quality. Controllable, non-toxic, low cost, easy for large-scale industrial production

Method used

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  • Preparation, purification and content detection methods for cerebroside
  • Preparation, purification and content detection methods for cerebroside
  • Preparation, purification and content detection methods for cerebroside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Bacteria used: Tuber melanosporum

[0069] 1. Preparation of truffle mycelium containing cerebroside in the first stage:

[0070] 1), slant strain cultivation: the formula of the slant strain culture medium adopted in the present embodiment is: glucose 20 grams / liter, magnesium sulfate 2 grams / liter, potassium dihydrogen phosphate 2 grams / liter, agar 20 grams / liter 1. 1.0 liter of potato extract; the sterilization conditions are: 121° C. for 20 minutes. The culture temperature is 30°C, and the culture time is 7 days;

[0071] 2), first-level liquid seed culture: the medium formula is glucose 300 g / L, peptone 60 g / L, yeast extract 60 g / L, magnesium sulfate 20 g / L, potassium dihydrogen phosphate 30 g / L; pH value 8.0; (2) culture conditions: culture temperature 35 ℃, shaker speed 250 rev / min;

[0072] The first-level liquid seed culture can be carried out by transferring the slant bacteria into the shake flask equipped with the first-level liquid seed medium. The cultu...

Embodiment 2

[0086] Chinese truffles (Tuber sinense), Indian truffles (Tuber indium), black spore truffles (Tuber melanosporum), white truffles (Tuber magnatum ) and summer truffles (Tuber aestivum ) were subjected to liquid fermentation at different scales. The composition and culture conditions of the fermentation medium are shown in Table 1. Slant strain cultivation, primary liquid seed cultivation and secondary liquid seed cultivation are the same as in Example 1. The detection method of cerebroside content is the same as in Example 1. The yields of cerebrosides in the obtained truffle fermentation liquid and mycelia are shown in Table 2.

[0087] Table 1. Medium composition and culture conditions of different fermentation scales

[0088]

[0089] Table 2. Comparison of cerebroside production under different fermentation scale conditions

[0090]

Embodiment 3

[0092] Change the concentration and culture temperature of the main components of the slant culture medium of Tuber melanosporum (Tuber melanosporum) (see Table 3), and investigate its influence on the production of cerebroside by liquid fermentation of Tuber melanosporum. Primary liquid seed cultivation, secondary liquid seed cultivation and liquid fermentation are the same as in Example 1. The detection method of cerebroside content is the same as in Example 1. The yields of cerebrosides in the obtained tuber melanosporum fermentation broth and mycelia are shown in Table 3.

[0093] Table 3. The effect of slant strain culture on the production of cerebroside by liquid fermentation of Niger truffle

[0094]

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Abstract

The invention discloses preparation, purification and content detection methods for cerebroside, which belong to the field of cerebroside preparation. The method for preparing the cerebroside comprises the step that: biological fermentation is carried out on tuber strains to obtain mycelium containing the cerebroside. In the method, the culture conditions and the culture medium composition in each step of liquid fermentation are optimized and screened, and the yield of the cerebroside is improved to the furthest degree. The invention also discloses a method for separating and purifying the cerebroside from the mycelium and detecting the content of the cerebroside. Tuber liquid is used for producing the cerebroside through fermentation. Compared with the existing cerebroside production method, the method has the advantages that the cost is low, the period is short, the operation is simple, the quality is controllable, the separation process is simple, and the like.

Description

technical field [0001] The invention relates to a method for preparing sphingolipid compounds, in particular to a method for preparing and purifying cerebrosides. The invention also relates to a method for detecting the content of cerebrosides, belonging to the field of cerebrosides preparation. Background technique [0002] Cerebroside is a kind of glycosphingolipid compound (also known as cerebroside or glycoceramide), which mainly exists in mammalian brain tissue, epidermis, heart, liver, and membrane tissue of red blood cells; in some large fungi and higher plants and It is also distributed in some marine organisms (sea discs, starfish, etc.). Its structural feature is composed of ceramide and sugar. Ceramide is a kind of amides formed by dehydrating the carboxyl group in long-chain fatty acids and the amino group of sphingosine (also known as long-chain base) through amide bonds. compound. Cerebroside is formed by linking ceramide with hexose through a hydroxyl group ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02C12N1/14C07H15/10C07H1/06G01N30/02C12R1/645
Inventor 汤亚杰李园园
Owner HUBEI UNIV OF TECH
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