A kind of refined polysaccharide of whole Ganoderma lucidum spore powder with significant auxiliary anti-tumor activity and its preparation method and application
A technology of Ganoderma lucidum spore powder and anti-tumor activity, which is applied in the direction of anti-tumor drugs, organic active ingredients, medical preparations containing active ingredients, etc., can solve the problems of accelerated death cases, patient exhaustion, etc., and achieve simplified process flow and impurity The effect of index improvement and production time saving
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Embodiment 1
[0070] Use 40kg of the whole Ganoderma lucidum spore powder residual residue that has been extracted from the whole Ganoderma lucidum spore oil in Example 2 of the invention patent CN 100593410C for standby, add 600L of purified water to the multi-energy extraction tank, and after boiling with steam, slowly add the above-mentioned For the residue, continue to stir and maintain a slight boil for 1 hour, let it settle and cool to below 50°C, take out the supernatant, filter it with a plate and frame, repeat the extraction of the residue once according to the above method, and combine the crude filtrate. The crude filtrate used 500nm tubular ceramic microfiltration membrane (CX-30-1016-4-19 type, 4 parallel connections, membrane area 0.952m 2 ) for clarification, the operating temperature is 50°C, the operating pressure is 0.35Mpa, the membrane surface flow rate is 4.5m / s, and the permeate flux is 360LMH. Repeat again. The clarified liquid was concentrated with a PT4040C2016 org...
Embodiment 2
[0073] Use 30kg of the whole Ganoderma lucidum spore powder residue that has been extracted from the whole Ganoderma lucidum spore oil in Example 2 of the invention patent CN 100593410C for standby, add 700L of purified water to the multi-functional extraction tank, and after boiling with steam, start the feed liquid pump under countercurrent forced circulation Slowly add the above residue, start the feed liquid pump at intervals of 10 minutes to force circulation for 5 minutes, maintain a slight boil for 1 hour, let it settle and cool to below 50°C, take out the supernatant, and use a filter bag filter with a filtration accuracy of 0.5 μm for coarse filtration , the residue was extracted once more according to the above method, and the crude filtrate was combined. The crude filtrate is finely filtered with a PP microporous folded filter element with a filtration accuracy of 0.1 μm. The fine filtrate was concentrated with a PT4040C2016 organic ultrafiltration membrane produced...
Embodiment 3
[0076] Determination of Protein and Polysaccharide Contents in Refined Polysaccharides of Whole Ganoderma Lucidum Spore Powder by UV-Vis Spectrophotometry
[0077] Determination of protein content: BCA method was used for determination, BCA protein concentration determination kit (Enhanced) (EnhancedBCA Protein Assay Kit) was purchased from Shanghai Beyontian Biotechnology Co., Ltd., and the detection was done according to the detailed instructions.
[0078] Determination of polysaccharide content: Determination by anthrone-sulfuric acid method, according to the steps in the "Chinese Pharmacopoeia" 2015 edition under the item of Ganoderma lucidum [Content Determination] Polysaccharides".
[0079] The protein content and polysaccharide content in the refined polysaccharide of the whole ganoderma lucidum spore powder prepared in Example 1 were measured according to the above method respectively, and the protein content of the refined polysaccharide of the whole ganoderma lucidum ...
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