A kind of medium for cultivating male stone pine and its application
A technology of public stone pine and culture medium, which is applied in the field of plant cultivation and can solve problems such as shortage of supply and the like
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Embodiment 1
[0027] The preparation of embodiment 1 culture medium
[0028] (1) Weigh each raw material component in proportion: MS medium: sucrose: carrageenan: carrot puree: peptone: banana puree: activated carbon=1L: 25g: 6g: 20g: 2g: 100g: 1.0g, wherein each liter of MS The medium contains 2mg of 6-BA, 1.0mg of NAA and 0.2mg of KT, and the preparation table of MS mother solution is as follows:
[0029]
[0030] (2) Add sucrose, carrageenan, carrot puree, peptone, banana puree and activated carbon into the MS medium, heat and stir evenly;
[0031] (3) Carry out high-pressure steam sterilization after subpackaging the material gained in step (2), then cool to solidification at room temperature;
[0032] (4) Place the material obtained in step (3) for 3 to 5 days in summer or 5 to 8 days in winter to remove the contaminated culture medium.
Embodiment 2
[0033] The cultivation of embodiment 2 male stone pine
[0034] (1) Put the explants or tissue-cultured aseptic seedlings of the red club stone pine obtained through cleaning and disinfection into the culture medium prepared in Example 1 to obtain 2 to 3 leaves and a height of 6 For bottle seedlings of ~7cm, the culture conditions are as follows: the growth temperature is 5 ~ 35°C (preferably 15 ~ 28°C), the light intensity is 1500lx ~ 4000lx (preferably 3500lx), and it is sterilized once every 15 ~ 30d (can be fumigated with formalin, Spray new gel or cooperate with ultraviolet light), wherein the specific illumination is: summer daytime illumination, winter nighttime illumination, the duration is 10-12h; this step is divided into two situations, when using naturally grown plants as explants It includes three steps of cleaning, disinfection and inoculation; when using tissue-cultured aseptic vaccines, perform aseptic inoculation directly, as follows:
[0035] a, natural grow...
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