A refined polysaccharide of Ganoderma lucidum fruiting body with significant auxiliary anti-tumor activity and its preparation method and application

A technology of Ganoderma lucidum fruiting body and anti-tumor activity, which is applied in the fields of extraction and separation technology and application and development of medicine and food, and can solve problems such as failure of normal production, clogging of liquid outlet, and excess

Active Publication Date: 2020-12-29
GUANGDONG YUEWEI EDIBLE FUNGI TECH
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Enzymatic extraction is a low-temperature and high-efficiency extraction technology. The yield of enzymatic extraction is very high, but enzymatic extraction also has significant defects, including: (1) The dissolution rate of impurities is proportional to the dissolution rate of effective substances, even exceeding the effective The multiple of the composition increases and introduces a large amount of impurities, which is not conducive to the purification and preparation of high-purity products. Therefore, enzymatic extraction technology is rarely used in varieties that require later purification and separation.
(2) The enzymatic extraction needs to crush the medicinal materials as much as possible to allow the enzyme and the substrate to form effective contact to the maximum extent, thereby increasing the product yield. However, sufficiently broken medicinal materials that meet the requirements of the enzymatic method bring a lot to the actual production. Trouble, such as blocking the liquid outlet and filter medium, so that the production cannot be carried out normally. For example, the water content of medicinal dregs is higher than that of decoction pieces, and the loss of target components due to the absorption of the extract by medicinal dregs is also large. also further increase
(3) In order to exert a good enzymatic hydrolysis effect, the common enzymes on the market need to adjust the pH value to the optimum pH value of the specific enzyme. For example, the optimum pH value of pectinase, cellulase, and pepsin are all If it is lower than 4, it will also cause some unknown impurities to change their chemical properties in a heating environment. Because the impurities are unknown, their reaction products with acids and bases may also be unknown, and effective monitoring cannot be formed in actual production. Therefore, it may bring certain clinical risks
(4) Furthermore, the applicant also found in the production practice that the taste and flavor of the enzymatic extract are significantly worse than those directly decocted in water, which may be due to the occurrence of flavor components such as amino acids, nucleotides, and volatile oils in the acid-base system. changed in nature
(5) Due to cost considerations, the enzymes that can be used in production are all enzymes that are directly put on the market after preliminary and simple purification after microbial fermentation. These enzymes are actually compound enzymes that are dominated by a certain enzyme, not in the biochemical field. The use of high-priced, high-purity single-specificity enzymes, the use of this complex enzyme in production, is also accompanied by the risk of target polysaccharides being decomposed

Method used

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  • A refined polysaccharide of Ganoderma lucidum fruiting body with significant auxiliary anti-tumor activity and its preparation method and application
  • A refined polysaccharide of Ganoderma lucidum fruiting body with significant auxiliary anti-tumor activity and its preparation method and application
  • A refined polysaccharide of Ganoderma lucidum fruiting body with significant auxiliary anti-tumor activity and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Cut the fruit body of Ganoderma lucidum into thin slices or pulverize it properly, add 10 times the weight of edible alcohol for reflux extraction for 2 times, each time for 2 hours, release the extract, and pass steam into the remaining fruit body residue to replace the alcohol residue as much as possible, and air to dry. After basically no alcohol odor, it is dried with hot air below 60°C to obtain pretreated fruit body residues.

[0094] 50kg of fruiting body residues for use, add 500L of purified water, heat to boiling with steam, stir at 15r / min and maintain a slight boil for 3h, turn off the steam, cool to below 50°C, extract the supernatant, and use a 0.8μm filter membrane to plate and frame After filtration, the residue was extracted twice according to the above method, and the crude filtrates were combined. 200nm tubular ceramic microfiltration membrane for crude filtrate (CX-30-1016-4-19 type, 8 parallel, membrane area 1.904m 2 ) for clarification, the operat...

Embodiment 2

[0097] Take 30kg of Ganoderma lucidum fruiting body residue that has been extracted with edible alcohol in advance and then dried for use, add 750L of purified water, heat it to boiling with steam, start the feed pump at intervals of 30min to force circulation for 5min, maintain slight boiling for 2h, turn off the steam, and cool to 50 Below ℃, extract the supernatant, use a bag filter with a filtration precision of 1 μm for coarse filtration, repeat the extraction of the residue once according to the above method, and combine the coarse filtrate. The crude filtrate is finely filtered with a pp microporous pleated filter element with a filtration accuracy of 0.22 μm, and the clarified liquid is collected. 60-100 mesh polyamide resin and D392 anion resin were purified and expanded according to the product instructions in advance, and were packed into columns with packing heights of 1.5m (BV=26.5L) and 30cm (BV=5.3L), respectively. In front) series D392 anion column (in the back...

Embodiment 3

[0100] Determination of protein and polysaccharide content in Ganoderma lucidum fruiting body refined polysaccharide by UV-Vis spectrophotometry, and calculation of polysaccharide retention rate, protein removal rate, and pigment removal rate

[0101] Determination of protein content: BCA method was used for determination. The BCA protein concentration determination kit (enhanced) (EnhancedBCA Protein Assay Kit) was purchased from Shanghai Biyuntian Biotechnology Co., Ltd., and the detection was done according to the detailed instructions.

[0102] Pigment measurement: directly measure the absorbance A value at 400nm with a spectrophotometer.

[0103] Determination of polysaccharide content: Determination by anthrone-sulfuric acid method, according to the steps in "Chinese Pharmacopoeia, 2015 Edition, Ganoderma lucidum [Determination of Content] Polysaccharides".

[0104] Take about 50 mg of the purified polysaccharide from the fruiting body of Ganoderma lucidum prepared in Ex...

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Abstract

The invention relates to ganoderma lucidum refined polysaccharides with significant assistant antitumor activity and a preparation method and application of the ganoderma lucidum refined polysaccharides. The refined polysaccharides is heteropolysaccharide which is mainly prepared from mannose, glucosamine, glucuronic acid, glucose, galactose, xylose and fucose in a molar ratio of (3 to 4) to 1 to(1.3 to 1.7) to (56 to 65) to (4 to 6) to (1.3 to 1.8) to (2.5 to 3.5), the absolute purity is greater than 52%, the weight average molecular weight is 7500-8500D, and the molecular weight distribution index is 1.3 to 1.5. The preparation method comprises the following steps of dynamically extracting by hot water, coarsely filtering to remove residues, carrying out fine filtration and clarifying,deproteinizing and decoloring by in-series resin beds, concentrating and removing impurities by an ultrafiltration membrane, and carrying out spray-drying or alcohol precipitation air drying. The method is intelligent and energy-saving, can solve industrial difficult problems, and has significant large scale advantage and good product safety, effectiveness, controllability and stability. The polysaccharides have significant functional activity in assisting in antitumor and are useful for the development of drugs for prevention and treatment of tumors and the development of food and health products in related fields.

Description

technical field [0001] The invention relates to a refined polysaccharide of Ganoderma lucidum fruiting body with significant auxiliary antitumor activity and a preparation method and application thereof, belonging to the fields of extraction and separation technology and application and development of medicine and food. Background technique [0002] The role of edible fungi on human health has received more and more attention from the international community. As one of the main active ingredients of edible fungi, edible fungus polysaccharide has become a research hotspot in the world health industry. A large number of research literatures show that edible fungus polysaccharides have various biological activities such as immune regulation, anti-tumor, anti-virus, anti-aging, anti-oxidation, lowering blood lipids, lowering blood pressure, and lowering blood sugar. At present, Asian countries led by China, Europe and the United States have carried out systematic research on po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L33/125A61P35/00
CPCY02P20/10
Inventor 帅欧陈地灵吴清平谢意珍杨柏华唐晓萃
Owner GUANGDONG YUEWEI EDIBLE FUNGI TECH
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