Preparation of alpha-androstanol and content detection method

A technology of androstanol and samples, applied in the direction of microorganism-based methods, biochemical equipment and methods, measuring devices, etc., can solve the problems of organic residues, high cost, low yield, etc., and achieve short cycle, low cost, and reliable quality control effect

Inactive Publication Date: 2009-07-29
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art and provide a new preparation method of α-androstanol, which is practical, simple to operate, short in cycle, controllable in quality, non-toxic, Low cost, easy large-scale industrial production, can well solve a series of problems such as organic residues, small scale, low output and high cost in the current chemical synthesis of α-androstanol

Method used

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  • Preparation of alpha-androstanol and content detection method
  • Preparation of alpha-androstanol and content detection method
  • Preparation of alpha-androstanol and content detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The strain used: Tuber melanosporum.

[0059] 1. Slant strain culture: the formula of the slant strain culture medium adopted in the present embodiment is: glucose 20 grams / liter, magnesium sulfate 2 grams / liter, potassium dihydrogen phosphate 2 grams / liter, agar 20 grams / liter, Potato extract 1.0 liter; sterilization conditions: 121°C, 20 minutes. The culture temperature is 30°C, and the culture time is 7 days;

[0060] 2. First-level liquid seed culture: the formulas of the first-level liquid seed and the second-level liquid seed medium are: glucose 50 g / L, peptone 10 g / L, yeast extract 10 g / L, magnesium sulfate 1 g / L, Potassium dihydrogen phosphate 2 g / L, pH 7.0. The first-level liquid seed culture can be carried out by transferring the slant bacteria into the shake flask equipped with the first-level liquid seed medium. The culture temperature is 30°C, the shaker speed is 150 rpm, and the culture time is 7 days;

[0061] 3. Secondary liquid seed cultivation: the...

Embodiment 2

[0070] Chinese truffle (Tuber sinense), Indian truffle (Tuber indium), black spore truffle (Tubermelanosporum), white truffle (Tuber magnatum) and summer truffle (Tuber aestivum) were subjected to liquid fermentation at different scales respectively. The composition and culture conditions of the fermentation medium are shown in Table 1. Slant strain cultivation, primary liquid seed cultivation and secondary liquid seed cultivation are the same as in Example 1. The detection method of α-androstanol content is the same as that in Example 1. The yields of α-androstanol in the obtained truffle fermentation liquid and mycelia are shown in Table 2.

[0071] Table 1. Medium composition and culture conditions of different fermentation scales

[0072]

Fermentation scale

sucrose

(g / L)

Peptone

(g / L)

Yeast extract

(g / L)

magnesium sulfate

(g / L)

dihydrogen phosphate

Potassium (g / L)

Liquid volume...

Embodiment 3

[0076] Change the concentration and culture temperature of the main components of the slant culture medium of Tuber melanosporum (Tuber melanosporum) to investigate its effect on the production of α-androstanol by liquid fermentation of Tuber melanosporum. Primary liquid seed cultivation, secondary liquid seed cultivation and liquid fermentation are the same as in Example 1. The detection method of α-androstanol content is the same as that in Example 1. The yields of α-androstanol in the obtained tubermelanosporum fermentation broth and mycelia are shown in Table 3.

[0077] Table 3. The effect of slant strain culture on the production of α-androstanol by liquid fermentation of truffle nigrosporum

[0078]

[0079] As can be seen from the test results, when the slant strains of the black spore truffle (Tuber melanosporum) were cultivated using the culture condition 2, compared with the other 4 groups of culture conditions, the α-androstane in the obtained mycelium or ferme...

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Abstract

The invention discloses a method for preparing alpha-androstanol, comprising the following steps: liquid fermentation is carried out on tuber bacterial strain to obtain zymotic fluid or mycelium containing the alpha-androstanol. The invention also discloses a method for detecting content of the alpha-androstanol in the zymotic fluid or the mycelium. In the invention, tuber liquid fermentation is adopted to produce alpha-androstanol, compared with chemosynthesis, the method of the invention has the advantages of low cost, short cycle, easy operation controllable quality and capability of preventing chemical residue, etc.

Description

technical field [0001] The invention relates to a preparation method of steroidal compounds, in particular to a preparation method of α-androstanol, and also relates to a quality control method for detecting the content of α-androstanol, which belongs to the field of microbial fermentation. Background technique [0002] α-Androstanol is a steroidal compound (also known as 5α-androst-16-en-3α-ol, 3α-hydroxy-5α-androst-16-ene, 16-[5α]androsten-3α-ol, androstenol ), the compound is contained in the saliva, testis, and liver of uncastrated boars, and is also found in human plasma, axillary sweat, and urine. The structural formula of α-androstanol is shown below: [0003] [0004] α-Androstanol has the function of regulating the menstrual cycle of women. Although it is still uncertain whether this compound has the effect of sex hormones that cause female sexual arousal, some companies have developed α-Androstanol into a commodity——Ferro Mongolian (pheromone), valuable. Rece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12N1/14G01N30/88C12R1/645
Inventor 汤亚杰王冠刘瑞桑李冬生
Owner HUBEI UNIV OF TECH
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