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Method for degrading residual tylosin in medicine slag

A technology for tylosin and degrading bacteria, which is applied in the field of using microorganisms to degrade residual tylosin in medicinal residues, can solve the problems of not being suitable for the thorough treatment of antibiotics, and the residual antibiotics have not been fundamentally eliminated, so as to achieve zero discharge, The effect of increasing the content

Inactive Publication Date: 2008-09-10
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Otker et al., Polubesova et al. used porous silicate minerals such as montmorillonite and zeolite to absorb residual tetracycline, enrofloxacin and other drugs in sewage, and then purified sewage, but this method did not fundamentally eliminate residual antibiotics. Only the existence site of antibiotics has changed, and it is not suitable for the thorough treatment of residual antibiotics in medicinal residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] A method for degrading residual tylosin in medicinal dregs, the method comprising the steps of:

[0036] 1) Preparation of soil bacterial suspension: Weigh 1g of the soil sample near the piled tylosin dregs, put it in a conical flask, add 100ml of phosphate buffer solution, shake well, put it in a constant temperature shaker, at 30°C , shake at 200r / min for 20min;

[0037] 2) Preparation of strain breeding medium: Mix the following raw materials by weight or volume to obtain strain breeding medium: 200 g of tylosin dregs, 10 g of peptone, 10 g of glucose, K 2 HPO 4 0.1g, FeSO 4 ·7H 2 O 0.2g, water 800ml;

[0038] 3) Preparation of strain domestication medium: Mix the following raw materials according to the weight ratio to obtain the strain domestication medium: ① primary domestication medium: tylosin medicinal residue and water in a ratio of 1:9 Preparation; ②Secondary acclimation medium: prepare tylosin dregs and water at a ratio of 2:8; ③Third-level acclimatizat...

example 2

[0048] A method for degrading residual tylosin in medicinal dregs, the method comprising the steps of:

[0049] 1) Preparation of soil bacterial suspension: Weigh 10g of the soil sample near the piled tylosin dregs, put it in a conical flask, add 200ml of phosphate buffer solution, shake well, put it in a constant temperature shaker, at 28°C , 200r / min oscillation for 40min;

[0050] 2) Preparation of strain breeding medium: Mix the following raw materials by weight or volume to obtain strain breeding medium: 200 g of tylosin dregs, 10 g of peptone, 10 g of glucose, K 2 HPO 4 0.1g, FeSO 4 ·7H 2 O 0.2g, water 800ml;

[0051] 3) Preparation of strain domestication medium: Mix the following raw materials according to the weight ratio to obtain the strain domestication medium: ① primary domestication medium: tylosin medicinal residue and water in a ratio of 1:9 Preparation; ②Secondary acclimation medium: prepare tylosin dregs and water at a ratio of 2:8; ③Third-level acclimat...

example 3

[0061] A method for degrading residual tylosin in medicinal dregs, the method comprising the steps of:

[0062] 1) Preparation of soil bacterial suspension: Weigh 5g of the soil sample near the piled tylosin dregs, put it in a conical flask, add 150ml of phosphate buffer solution, shake well, put it in a constant temperature shaker, at 35°C , 200r / min shaking for 30min;

[0063] 2) Preparation of strain breeding medium: Mix the following raw materials by weight or volume to obtain strain breeding medium: 200 g of tylosin dregs, 10 g of peptone, 10 g of glucose, K 2 HPO 4 0.1g, FeSO 4 ·7H 2 O 0.2g, water 800ml;

[0064] 3) Preparation of strain domestication medium: Mix the following raw materials according to the weight ratio to obtain the strain domestication medium: ① primary domestication medium: tylosin medicinal residue and water in a ratio of 1:9 Preparation; ②Secondary acclimation medium: prepare tylosin dregs and water at a ratio of 2:8; ③Third-level acclimatizati...

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Abstract

The invention relates to a method for degrading residual tylosin in herb residues, in particular to a method for degrading residual tylosin in herb residues by utilizing microorganism, which mainly comprises the steps of preparation of soil fungi suspension, preparation of strain breeding cultural medium, preparation of strain domestication cultural medium, preparation of strain screening cultural medium, preparation of stain activation and rejuvenation cultural medium, preparation of drug residues fermentation cultural medium, screening of degrading strain and so on. The invention provides a compound bacterium that can be screened and cultured to degrade the residual tylosin in herb residues, while strains for degrading the residual tylosin in herb residues can be acquired by being separated and purified from the compound bacterium. Then the strains can be prepared to be corresponding herb residue cultural medium, which can degrade the residual tylosin in herb residues after being accessed the fermentation of the degrading bacteria.

Description

technical field [0001] The invention relates to a method for degrading residual tylosin in medicinal residues, in particular to a method for using microorganisms to degrade residual tylosin in medicinal residues. Background technique [0002] Due to the high organic matter content and rich nutrition of antibiotic dregs, simple drying or stacking can easily cause secondary fermentation, turn black in color, and produce musty odor. In the 1990s, people focused on the treatment of antibiotic dregs on the drying technology. In 1991, the drying process of antibiotic dregs of Shandong Lukang Pharmaceutical Group Co., Ltd. was successful. In 1996, Shandong Lukang Feed Factory developed a centrifuge combined with a fluidized bed to dry penicillin wet residue, which reduced the drying cost by 30%. [0003] With the successful development of various dregs drying equipment, several domestic units have carried out research on the use of antibiotic dregs as high-protein feed raw materi...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/20
Inventor 马玉龙张作义刘宁普谢丽
Owner NINGXIA UNIVERSITY
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