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Method for degrading residual tylosin in medicine slag

A technology of tylosin and degrading bacteria, which is applied in the field of microbial degradation of tylosin residues in medicine dregs, can solve the problems of not fundamentally eliminating residual antibiotics and not being suitable for thorough treatment of antibiotics, and achieve zero discharge, The effect of increasing content

Inactive Publication Date: 2011-04-06
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Otker et al., Polubesova et al. used porous silicate minerals such as montmorillonite and zeolite to absorb residual tetracycline, enrofloxacin and other drugs in sewage, and then purified sewage, but this method did not fundamentally eliminate residual antibiotics. Only the existence site of antibiotics has changed, and it is not suitable for the thorough treatment of residual antibiotics in medicinal residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] A method for degrading residual tylosin in medicine residue, the method comprising the following steps:

[0036] 1) Preparation of soil bacterial suspension: Weigh 1g of the soil sample near the pile of tylosin dregs, place it in a triangular flask, add 100ml of phosphate buffer solution, shake well, and place it in a constant temperature shaker at 30°C , 200r / min oscillation for 20min;

[0037] 2) Preparation of strain breeding medium: Mix the following materials in weight or volume to obtain strain breeding medium: 200g tylosin residue, 10g peptone, 10g glucose, K 2 HPO 4 0.1g, FeSO 4 ·7H 2 O 0.2g, water 800ml;

[0038] 3) Preparation of strain acclimation medium: Mix the following raw materials according to the weight ratio to obtain strain acclimation medium: ① Primary acclimation medium: Tylosin dregs and water in a ratio of 1:9 Preparation; ②Secondary domestication medium: tylosin dregs and water are prepared at a ratio of 2:8; ③third-level domestication medium: tylosin...

example 2

[0048] A method for degrading residual tylosin in medicine residue, the method comprising the following steps:

[0049] 1) Preparation of soil bacterial suspension: Weigh 10g of the soil sample near the tylosin dregs, place it in a triangular flask, add 200ml of phosphate buffer solution, shake well, place it in a constant temperature shaker at 28°C , 200r / min oscillation for 40min;

[0050] 2) Preparation of strain breeding medium: Mix the following materials in weight or volume to obtain strain breeding medium: 200g tylosin residue, 10g peptone, 10g glucose, K 2 HPO 4 0.1g, FeSO 4 ·7H 2 O 0.2g, water 800ml;

[0051] 3) Preparation of strain acclimation medium: Mix the following raw materials according to the weight ratio to obtain strain acclimation medium: ① Primary acclimation medium: Tylosin dregs and water in a ratio of 1:9 Preparation; ②Secondary domestication medium: tylosin dregs and water are prepared at a ratio of 2:8; ③third-level domestication medium: tylosin dregs and ...

example 3

[0061] A method for degrading residual tylosin in medicine residue, the method comprising the following steps:

[0062] 1) Preparation of soil bacteria suspension: Weigh 5g of the soil sample near the tylosin residue, place it in a triangular flask, add 150ml of phosphate buffer solution, shake well, place it in a constant temperature shaker at 35°C , 200r / min oscillation for 30min;

[0063] 2) Preparation of strain breeding medium: Mix the following materials in weight or volume to obtain strain breeding medium: 200g tylosin residue, 10g peptone, 10g glucose, K 2 HPO 4 0.1g, FeSO 4 ·7H 2 O 0.2g, water 800ml;

[0064] 3) Preparation of strain acclimation medium: Mix the following raw materials according to the weight ratio to obtain strain acclimation medium: ① Primary acclimation medium: Tylosin dregs and water in a ratio of 1:9 Preparation; ②Secondary domestication medium: tylosin dregs and water are prepared at a ratio of 2:8; ③third-level domestication medium: tylosin dregs and ...

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Abstract

The invention relates to a method for degrading residual tylosin in herb residues, in particular to a method for degrading residual tylosin in herb residues by utilizing microorganism, which mainly comprises the steps of preparation of soil fungi suspension, preparation of strain breeding cultural medium, preparation of strain domestication cultural medium, preparation of strain screening cultural medium, preparation of stain activation and rejuvenation cultural medium, preparation of drug residues fermentation cultural medium, screening of degrading strain and so on. The invention provides acompound bacterium that can be screened and cultured to degrade the residual tylosin in herb residues, while strains for degrading the residual tylosin in herb residues can be acquired by being separated and purified from the compound bacterium. Then the strains can be prepared to be corresponding herb residue cultural medium, which can degrade the residual tylosin in herb residues after being accessed the fermentation of the degrading bacteria.

Description

Technical field [0001] The invention relates to a method for degrading residual tylosin in drug residues, in particular to a method for using microorganisms to degrade the residual tylosin in drug residues. Background technique [0002] Due to the high organic content and rich nutrition of antibiotic medicine residue, it is easy to cause secondary fermentation, blackening of color, and mildew odor when it is simply dried or stacked. In the 1990s, the treatment process of antibiotic dregs was mainly focused on the research of drying technology. In 1991, Shandong Lukang Pharmaceutical Group Company's antibiotic residue drying process was successful. In 1996, Shandong Lukang Feed Factory developed a centrifuge combined with a fluidized bed to dry the wet residue of penicillin, which reduced the drying cost by 30%. [0003] With the successful development of various medicine residue drying equipment, several domestic units have carried out research on the use of antibiotic medicine r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02A62D101/20
Inventor 马玉龙张作义刘宁普谢丽
Owner NINGXIA UNIVERSITY
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