Method for constructing soil fungal antagonism model

A construction method and fungal technology, which are applied in the field of soil fungal antagonistic model construction, can solve the problems of differences in soil fungal antagonistic functions, and it is difficult to study the influencing factors of fungal antagonistic functions, etc. wide effect

Inactive Publication Date: 2009-04-22
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

It is difficult to study the real influence factors of fungal antagonistic function in the presence of these influence factors
However, in actual experiments, it is almost impossible to find soil samples with exactly the same background information (i.e. soil type, farming method, crop type, fertility level, etc.), but with differences in the antagonistic functions of soil fungi.

Method used

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  • Method for constructing soil fungal antagonism model
  • Method for constructing soil fungal antagonism model

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Construction of an antagonistic model of soil fungi

[0021] The construction method of the soil fungal antagonism model: the collected soil samples are subjected to high pressure and high temperature to establish a soil fungal antagonism model in which the soil anti-fungal ability gradually decreases, and the construction is carried out through the following steps:

[0022] 1) collecting soil samples;

[0023] 2) High pressure and high temperature treatment of soil samples;

[0024] 3) Detect fungal antagonistic function;

[0025] The soil sample in the step 1) is soil without plant diseases; in the step 2), the soil sample is divided into 4 parts, 1 part is an untreated blank control, and the other 3 parts are respectively in an autoclave at 100 ° C, Treat at 110°C and 121°C for 4 minutes; Step 3) Insert the soil samples after high temperature and high pressure treatment into the agarose mycelia blocks of Fusarium graminearum, and test the mycelium after culturing a...

Embodiment 2

[0036] The difference from Example 1 is:

[0037] The soil sample used was black soil collected by Heilongjiang Hailun Ecological Experimental Station, and then the model was constructed according to the operation steps of Example 1.

[0038] After verification, it can be known that in the black soil collected by the Hailun Ecological Experiment Station in Heilongjiang, it was found that the bacteria represented by the clones with the highest similarity to Alphaproteobacteria, Betaproteobacteria, Burkholderia, Pseudomonas, Bacillus, and Xanthomonas played a decisive role in the antagonistic function of soil fungi.

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Abstract

The invention relates to biological technology, in particular to a method used for establishing a soil fungi antagonistic model. The method comprises the concrete steps as follows: the collected soil sample is disposed under high temperature and high pressure so as to establish the soil fungi antagonistic model with the soil fungi-inhibiting capability being gradually reduced. The soil fungi antagonistic model is established by the following steps: 1) sampling soil samples; 2) disposing the soil samples by high pressure and high temperature; 3) detecting the fungi antagonistic model. The method used for establishing model is simple, convenient and feasible, can be operated in common laboratories, and has short time consumption; furthermore, the model can be applied to different soil samples, can be used for researching the fungi antagonistic function of different soils and discovering specific fungi-inhibiting microorganism of the soil, can research the bacterial community structure of the soil and can research the relationship between the community structure composition of other microorganisms such as fungi, actinomycete and the like and the fungi antagonistic function.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for constructing a soil fungus antagonism model. Background technique [0002] Dobbs C.G. and Hinson W.H. discovered in 1953 that natural healthy soil can control the growth and reproduction of soil fungi (especially pathogenic fungi) to a certain extent, that is, it has fungal antagonistic functions. The fungal antagonistic function of natural healthy soil is the same as the carbon-nitrogen cycle that soil microorganisms participate in. It is another important biological indicator of soil quality and biological function, and has positive ecological significance for inhibiting soil-borne diseases of crops. At present, it is receiving extensive attention from soil scientists and soil microbiologists all over the world. [0003] There are seven hypotheses about the mechanism of soil fungal antagonistic function, including (1) antifungal antibiotic hypothesis; (2) Fe ion chelation competit...

Claims

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Application Information

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IPC IPC(8): G01N33/00C12Q1/02
Inventor 张惠文吴敏娜李旭李新宇苏振成张成刚
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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