A kind of bacterial strain and application for preventing and treating soil-borne fungal diseases of continuous cropping peanut

A soil-borne fungus and peanut technology, applied in the field of intensive agricultural production, can solve the problems of high application cost, large dosage, unstable control effect in the field, etc., achieve low cost, reduce the base of pathogenic bacteria, and significantly increase the effect of production

Active Publication Date: 2019-08-13
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in view of the fact that bio-control bacteria are living organisms, it is difficult to survive directly in organic fertilizers without intensive induction, resulting in unstable control effects in the field
In addition, the existing organic fertilizers for biological control generally use a large amount (>200 kg / mu), and the application cost is relatively high

Method used

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  • A kind of bacterial strain and application for preventing and treating soil-borne fungal diseases of continuous cropping peanut
  • A kind of bacterial strain and application for preventing and treating soil-borne fungal diseases of continuous cropping peanut
  • A kind of bacterial strain and application for preventing and treating soil-borne fungal diseases of continuous cropping peanut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Collect the peanut rhizosphere soil of a continuous peanut field in the red soil area for related experiments. The specific steps are:

[0034] (1) Soil sample treatment: Prepare sterilized enrichment medium (Ingredients: fine powder chitin 2.5g, MgSO 4 ·7H 2 O 0.5g, K 2 HPO 4 0.7g, KH 2 PO 4 0.3g, FeSO 4 ·7H 2 O 0.01g, distilled water 1000ml), divide 20ml into a sterile Erlenmeyer flask, and add 1.0g of fresh soil sample to the Erlenmeyer flask, mix, and incubate at 150r / min for 48h at room temperature to obtain an enriched culture solution.

[0035] (2) Coating separation: Pipette 1mL filtrate into 9.0mL sterile water, and dilute 3 times to obtain a dilution of 10 -4 Dilute the sample solution in a gradient. Spread 0.02 mL of the soil dilution solution on the plate separation medium and incubate at 28°C for 4-7 days. Observe and pick out the bacterial strains producing transparent circles.

[0036] (3) Cultivation and purification: pick a single colony and draw a line on a pl...

Embodiment 2

[0042] Example 2: Bacteriostatic experiment on various soil-fax type pathogens

[0043] Use the strain with the deposit number CGMCC NO.11539 for antibacterial experiment: first activate the strain, inoculate the pathogenic fungus in the center of the PDA plate, punch holes on the edge of the pathogenic colony with a diameter of 5mm to make a bacterial cake, and then connect it Go to the center of the PDA plate, and then inoculate the bacterial strains to be tested about 2.5cm away from the pathogens around the petri dish. Three replicates of each bacterial strain, culture for one week at 28°C, observe whether a zone of inhibition is produced, and determine the colony diameter of pathogenic fungi by the cross method , Calculate the inhibition rate. The selected pathogens are: Rhizoctonia solani, Fusariumsolani, Fusarium oxysporum f.sp.diath, Verticilliumdahliae Kleb, such as figure 2 — Figure 5 It was found that the strains had good antibacterial effects on the above-mentioned ...

Embodiment 3

[0046] Example 3: Greenhouse in vivo control experiment

[0047] The peanut root rot pathogen (Fusarium solani rot) was activated in the PDA medium and cultured at 28°C for 7 days. The spores of the pathogen on the medium were washed with sterile water. The bacterial solution was filtered with sterilized filter paper to remove the hyphae. Dilute the filtrate with appropriate distilled water to a spore concentration of 1×10 7 A / mL suspension, set aside.

[0048] Add the tested biocontrol strains to the culture medium containing no fungal hyphae inducer (beef extract 3%, peptone 8%) for liquid fermentation (26°C, 72h, 200rpm), and then inoculate decomposed pig manure at a rate of 5% Compost the substrate, place it in a constant temperature box (28°C) for 5 days, and use it as bacterial agent A for use.

[0049] At the same time, according to the same method as above, except that the inactivated fungal mycelial inducer (3%) is added to the liquid medium to carry out antibacterial enhan...

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Abstract

The invention relates to a bacterial strain and a bacterial agent thereof for preventing and treating fungal diseases of peanut soil in continuous cropping. The bacterial strain is a kind of Pseudomonas protegens, and the preservation number is CGMCC No.11539. The 16s rRNA base sequence of the strain is shown in SEQ ID No:1. The strain of the present invention produces chitinase (degrades mycelium cell wall of pathogenic fungus) with high activity, and has remarkable degrading ability to mycelium of various soil-borne fungal pathogens such as Fusarium, Rhizoctonia, Verticillium and the like. The seed dressing dosage of the bacterial agent of the present invention is generally only 20-30 kg / mu, which can effectively prevent and control frequent soil-borne fungal diseases such as peanut root rot, blight, and silkworm in dry land, with a control effect of more than 55%. It has the advantages of small dosage, wide antibacterial spectrum, and significant yield-increasing effect, and can effectively reduce the impact of soil-borne diseases on the yield loss of continuous cropping ground peanuts.

Description

Technical field [0001] The invention relates to a strain used for continuous cropping of peanut soil facsimile disease and a preparation method thereof, belonging to the technical field of agricultural intensive production, and is dedicated to overcoming the high incidence of soil facsimile disease of continuous cropping peanut. Background technique [0002] Peanuts are an important oil crop in my country, and the annual sown area accounts for about 35% of the total sown area of ​​oil crops. The southern red soil hilly area is one of the main peanut producing areas in my country. Compared with the main producing areas in North China, the output of peanuts in the southern red soil area is relatively low (the average output is only about 60% of that in the North China area). The climate conditions and soil fertility are related to the serious obstacles to peanut continuous cropping in the southern red soil area. Due to the low fertility of red soil dry slope land and severe seasona...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 李孝刚王兴祥李忠佩丁昌峰周志高
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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