Application of trans-aconitic acid in controlling agricultural pests

A technology of trans aconitic acid, molecular formula, used in the field of chemical medicine and biopesticide application

Active Publication Date: 2013-12-04
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of trans-aconitic acid in controlling agricultural pests
  • Application of trans-aconitic acid in controlling agricultural pests
  • Application of trans-aconitic acid in controlling agricultural pests

Examples

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Embodiment 1

[0023] Example 1: Bioassay of trans-aconitic acid (TAA) on root-knot nematode (Meloidogyne incognita)

[0024] The roots of tomato plants infested by Meloidogyne incognita were collected from the greenhouse of Huazhong Agricultural University in Hongshan District, Wuhan City, Hubei Province, China. The tomato roots were washed with sterile distilled water, and the egg masses of the root-knot nematode were removed from the roots and placed on ice. In a Petri dish, use 1% H in a sterile operating bench 2 o 2 And 0.025% KI disinfection twice, each 15min. Transfer the sterilized egg mass to another sterilized petri dish, then wash it with sterile distilled water 4-5 times for 5 minutes each time, add 20 mL of sterile distilled water, and incubate in a 25°C incubator for 3-5 days.

[0025] The hatched 2nd instar Meloidogyne incognita can be used as the target organism for the bioassay. Bioassays were carried out on a commonly used 96-well plate: about 40 2nd-instar Meloidogyne i...

Embodiment 2

[0029] Example 2: Bioassay of trans-aconitic acid (TAA) on soybean cyst nematode (Heterodera glycine Ichinohe)

[0030] Isolation of cysts by floating method from soil where soybean cyst nematode (Heterodera glycine Ichinohe) is seriously infested, and the cysts were placed in ZnSO 4 Soak in the solution for 24 hours, and after the soybean cyst nematode larvae hatch at room temperature, dilute until there are about 200 2nd instar larvae in every 1ml suspension under microscope inspection, and set aside.

[0031] The hatched 2nd instar soybean cyst nematode (Heterodera glycine Ichinohe) was used as the target organism of the bioassay. Bioassay of virulence was carried out on a commonly used 24-well plate: about 200 2nd-instar soybean cyst nematode (Heteroderaglycine Ichinohe) larvae were inhaled into each well, TAA of known concentration was added (see Table 2 for specific concentration), blank treatment was added, etc. The amount of sterile deionized water, the total volume o...

Embodiment 3

[0035] Embodiment 3: Trans-aconitic acid (TAA) is to the bioassay of sweet potato stem nematode (Ditylenchus destructor)

[0036] Sweet potato stem nematode (Ditylenchus destructor) was preserved by the State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University. The bioassay of virulence was carried out on a commonly used 24-well plate: about 50 sweet potato stem nematodes (Ditylenchus destructor) were inhaled into each well, TAA with a known concentration was added (see Table 3 for the specific concentration), and an equal amount of sterile detox was added to the blank treatment. Ionized water, the total volume of each hole is 2ml, make up the total volume with sterile deionized water, count the number of dead heads after 3 days, the experimental results are shown in Table 3.

[0037] Table 3. TAA bioassays against sweet potato Ditylenchus destructor

[0038]

[0039] The bioassay results showed that: TAA had a good toxic effect on D. sweetpotato ...

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Abstract

The invention belongs to the technical field of biological pesticides and relates to application of trans-aconitic acid in controlling plant pathogenic nematodes and harmful insects. According to bioassay, the trans-aconitic acid has the activity of obviously killing meloidogyne incognita chitwood, heterodera glycine ichinohe and ditylenchus destructor, and the activity of suppressing the growth of cotton bollworms and houseflies; the concentration of the trans-aconitic acid used for the meloidogyne incognita chitwood is 100-1000 [mu]g/mL, preferably 500 [mu]g/mL, with the death rate of 85.5 percent; 200-1000 [mu]g/mL for the heterodera glycine ichinohe, preferably 500 [mu]g/mL, with the death rate of 99.2 percent; 500 [mu]g/mL for the ditylenchus destructor with the death rate of 53.4 percent; 10-1000 [mu]g/mL for the cotton bollworms, preferably 200 [mu]g/mL, with the growth suppressing rate of 34.0 percent; and 10-1000 [mu]g/mL for the houseflies, preferably 200 [mu]g/mL, with the growth suppressing rate of 13.4 percent. The trans-aconitic acid is expected to become a pesticide composition for controlling plant pathogenic nematodes, cotton bollworms and houseflies.

Description

technical field [0001] The invention belongs to the technical field of application of chemical drugs and biological pesticides. The invention relates to the application of trans-aconitic acid in preventing and controlling agricultural pests, and the application includes the application in preventing and controlling plant pathogenic nematodes, cotton bollworms and houseflies. Background technique [0002] Trans-aconitic acid (TAA for short) is a tricarboxylic acid compound with the chemical formula C 6 h 6 o 6 , with a molecular weight of 174Da, and its structural formula is shown below. [0003] [0004] In the natural environment, TAA mainly exists in plants, and many plants contain TAA. For example, TAA is the dominant organic acid in corn (Clark et al., 1973); in crested wheat grass, the content of TAA can account for 6% of its dry weight (Schwartz et al., 1988 ); a large amount of TAA exists in the leaves and roots of barnyardgrass (Watanabe et al., 1997). [00...

Claims

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Application Information

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IPC IPC(8): A01N37/06A01P5/00A01P7/04
Inventor 孙明曹诗云聂相涛都萃颖彭东海阮丽芳
Owner HUAZHONG AGRI UNIV
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