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A cultivation method and technology of in vitro cultivation, applied in animal husbandry and other fields, to achieve the effects of rapid growth, strong vitality and easy cultivation
Inactive Publication Date: 2017-07-25
GANSU AGRI UNIV
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In scientific research and practice, not enough materials are the main constraints for the development of the nematode's physiological and biochemical characteristics, pathogenic mechanism, genetic characteristics, drug screening and control technology. The culture technique of the nematode population is particularly important
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[0018] The specific embodiment The present invention utilizes the method for fungus cultivation D. destructor, and its specific operation steps are as follows:
[0019] 1. Production of PDA medium: Weigh 200 g of washed and peeled potato cubes, chop them, add 1000 mL of water and boil for half an hour, filter the potato cubes with gauze, add 15 g of glucose, 15 g of agar, add water Make up 1000 mL and heat to melt the agar. Divide into Erlenmeyer flasks, and sterilize at 121°C for 20 minutes after stoppering. When the temperature of the culture medium drops to about 45 ℃, divide it into 9cm diameter petri dishes, with 10-15mL of culture medium in each dish.
[0020] 2. Fusarium culture
[0021] Fusarium ( Fusarium spp.) or preserved Fusarium strains were inoculated on the PDA plate medium on the ultra-clean workbench, and cultivated at a constant temperature of 25°C. After the mycelium is covered with the petri dish, use a sterile puncher to punch out the Fusarium fungus...
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Abstract
The invention discloses a ditylenchus destructor isolated culture method. The method includes following steps: step 1, culturing fusarium on a culture medium to grow mycelia; step 2, culturing ditylenchus destructor in the culture medium growing with the mycelia. According to habitual nature that the ditylenchus destructor eats fungi, culturable fungi which the ditylenchus destructor likes to eat are selected and cultured in a targeted manner, the potato sucrose agar (PDA) culture medium is adopted to culture the fungi, and the mycelia of the fungi are utilized to culture the ditylenchus destructor, so that the problem of controllable and sustainable culture of the ditylenchus destructor is solved, and isolated culture of the ditylenchus destructor is realized successfully and effectively.
Description
technical field [0001] The invention relates to a method for cultivating Dylodes rot nematode by using fungi, which belongs to plant pathogenic nematode cultivation. Background technique [0002] Demia destructor ( Ditylenchus destructor ) is a globally important plant pathogenic nematode, widely distributed in the United States, Canada, Peru, Japan, New Zealand, South Africa, the Netherlands, Norway, Russia, Iran, Australia and other countries and regions, causing serious harm. In my country, it is mainly distributed in Beijing, Hebei, Henan, Anhui, Shandong, Jiangsu, Gansu, Yunnan and other places. [0003] D. destructor mainly affects sweet potatoes and potatoes. On sweet potatoes, D. destructor mainly causes symptoms such as bran core, hollowness and rot in sweet potato tubers, which can generally lead to a 20% to 50% reduction in yield, and in severe cases, it can reach more than 80%, or even no harvest. It can also cause rotten cellars during storage. It is an import...
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