The invention relates to aquaculture feed, in particular to litopenaeus vannamei compound feed special for winter shed cultivation and a preparation method of the feed. The feed comprises a protein source raw material, a fat source raw material, a sugar source raw material, an antioxidant, a phagostimulant, composite vitamin and composite mineral salt. The invention provides the litopenaeus vannamei compound feed special for winter shed cultivation and the preparation method of the feed, with the advantages of high survival rate, high growth speed, high stability in water, high ingestion speed, convenience for use and low environmental pollution, so as to solve the problems of low growth speed, serious disease, low survival rate and the like of the litopenaeus vannamei during winter shed cultivation at low temperature and promote the healthy development of the litopenaeus vannamei cultivation industry.