Biological intelligence fungus block formula for preventing and treating nematodes and preparation method of biological intelligence fungus block

An intelligent, bacterial block technology, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve problems such as environmental pollution, wide distribution, human and animal health hazards, etc.

Inactive Publication Date: 2015-04-29
海南博士威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Root nematodes are a kind of resident endoparasitic nematodes, which infect the roots of plants and produce root knots, which are characterized by wide distribution and great harm. On the one hand, they produce some chemical substances to stimulate the plant host to form giant cells, forming a large number of root knots in the root system, affecting The root system absorbs water and nutrients; on the other hand, it causes a large number of wounds due to its infection, which is conducive to the secondary infection of soil pathogens, and this compound infection aggravates the loss of crop yield and the decline of quality
[0003] At present, the prevention and control of root nematodes is mainly the method of using chemical nematicides, but as people pay more and more attention to environmental issues and pollution-free food production today, many highly toxic substances that seriously pollute the environment and are harmful to human and animal health , high-residual and triple-toxic chemical nematicides have been gradually banned

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Preparation of the biological intelligent bacterial block of the present invention.

[0018] Strain used: Strain ACCC11084 is Bacillus megaterium Bacillus megaterium de Bary ; strain ACCC10632 is Bacillus subtilis Bacillus subtilis (Ehrenberg) Cohn; Paecilomyces lilacinus Paecilomyces lilacinus (Thom.) Samson; All three strains were purchased from China Agricultural Microorganism Culture Collection and Management Center.

[0019] First, prepare yeast powder of strain ACCC11084: liquid fermentation medium formula: peptone-5.2g, NaCl-5.5g, MnSO 4 ·H 2 O-0.006g, hydroxyene adenine-0.3g, ene adenine-0.3g, beef extract-3.3g, agar-0.2g, adjust the pH value to 7.0, and make up to 1000g with water. Configure the required amount of fermentation medium according to the above ratio, then insert the strains into the liquid fermentation medium under aseptic conditions, shake and culture on a shaker at 30°C for 72 hours (the number of effective viable bacteria rea...

Embodiment 2

[0024] Embodiment 2: Determining the biological activity of the biological intelligent bacterial block of the present invention.

[0025] Prepared according to the preparation method of the present invention: the seedling bacterium block containing only bacterial strain ACCC11084, the seedling bacterium block containing only bacterial strain ACCC10632, the seedling bacterium block containing only bacterial strain ACCC32001 and the biological intelligence bacterium block of the present invention.

[0026] Cucumber, peanut and soybean seedlings were raised with the four kinds of bacteria blocks prepared above, and finally transplanted to the greenhouse or field where root nematodes occurred seriously, and the incidence rate was observed after 15 days.

[0027] Table 1 shows the control effects of four kinds of bacterial blocks used to control cucumber root-knot nematode after 15 days of experiment.

[0028] handle name ACCC11084 ACCC10632 ACCC32001 Bio-intellig...

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PUM

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Abstract

The invention relates to a biological intelligence fungus block for preventing and treating stubby root nematodes. The biological intelligence fungus block is prepared by fermenting and drying three bacterial strains: ACCC11084: bacillus megaterium bacillus megaterium de Bary, ACCC10632: bacillus subtilis (Ehrnberg) Cohn and ACCC32001: paecilomyces lilacinus (Thom.)Samson to obtain bacterial strain mother powder, and then mixing and pressing with carrier auxiliary materials at the ratio. The intelligence fungus block can be used for growing seedlings of multiple vegetables, flowers and economic crops, is capable of preventing nematodes from damaging plants during the seedling stage, still can play good prevention and treatment roles on the stubby root nematodes after being transplanted into a field or a greenhouse, and especially has a good control effect on soybean cyst nematodes, cucumber root knot nematodes and peanut root knot nematodes.

Description

technical field [0001] The invention relates to a seedling-raising bacterial block, in particular to a formula and a preparation method of a living biological intelligent bacterial block which can be used for preventing and treating plant root nematode diseases. Background technique [0002] Root nematodes are a kind of resident endoparasitic nematodes, which infect the roots of plants and produce root knots, which are characterized by wide distribution and great harm. On the one hand, they produce some chemical substances to stimulate the plant host to form giant cells, forming a large number of root knots in the root system, affecting The root system absorbs water and nutrients; on the other hand, because of its infection, it causes a large number of wounds, which is conducive to the secondary infection of soil pathogens. This compound infection aggravates the loss of crop yield and the decline of quality. [0003] At present, the prevention and control of root nematodes i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P5/00
Inventor 吴景霄唐睿任承才杜荣锴吴淑科王昌显严崇泽
Owner 海南博士威生物科技有限公司
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