Compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil
A compound microbial inoculant and compound microbial technology, which is applied in the field of soil environment remediation, can solve the problems of high operation and maintenance costs, high consumption of water resources, large maintenance workload, etc., achieving a wide range of applications, improving soil fertility, and a simple process. easy-to-control effects
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[0064] (1) Preparation of bacterial suspension:
[0065] (1-i) Firstly, the five microbial strains of Bacillus megaterium, Pseudomonas stutzeri, Lactobacillus delbrueckii, Rhizobium radiata, Alcaligenes faecalis, and Candida tropicalis were respectively cultured in test tubes and shaken flasks. Cultivation and seed tank cultivation, and then accessing the fermenter liquid at a ratio of 5% to 10% by volume for cultivation, the cultivation temperature of each level of cultivation is 25°C to 35°C (such as 25, 30 or 35°C), and the cultivation time is 24 hours to 48 hours (such as 24, 30, 36, 42 or 48 hours), thereby respectively obtaining the respective viable counts of the five microbial strains greater than or equal to 10 8 / mL (eg 1X10 8 / g, 5X10 8 / g, 1X10 9 / g or 5X10 9 / g) of the first bacterial suspension; and
[0066] (1-ii) Carry out step-by-step liquid static anaerobic expansion culture of Lactobacillus delbrueckii, the culture temperature of each level of culture i...
Embodiment 1
[0109] Example 1 Field experiment on 500 mu of saline-alkali land in Yingnan Village, Dali County, Weibei area
[0110] (1) Test site
[0111] The landform of the test is a depression outside the embankment of the Yellow River, which has not been cultivated for eight years, with reeds growing on it, and thick salt (white) on the surface. The soil types are mainly fluvo-aquic soil and saline soil, the soil salt content is more than 1%, the chemical type is mainly chloride, and a small part is sulfate and silicate. Soil anion type is Cl - 、HCO 3- , SO 4 2- , cation type Na + , Ca 2+ 、K + . Soil organic matter is low, stickiness is high, and crops are not resistant to growth (see figure 1 , which shows the untreated salinized soil in Weibei region).
[0112] (2) Test procedure
[0113] (a) Bacillus megaterium, Pseudomonas stutzeri, Rhizobium radiata, Alcaligenes faecalis, and Candida tropicalis were respectively cultured in test tubes, shake flasks, and seed tanks in s...
Embodiment 2
[0131] Example 2 Microbiological remediation test of 100 mu of saline-alkali soil in Nanmulin County, Shigatse, Tibet
[0132] (1) Test site
[0133] The Shigatse region of Tibet is located on the Tibetan plateau at an altitude of 4,100 meters. The annual precipitation is only 260 millimeters, the evaporation is as high as 2,200 millimeters, and the annual frost-free period is only 120 days. %), the top 50cm of soil is sandy soil with extremely poor water retention, high salinity, and about 50cm of permafrost below the surface for plant roots to grow downward. image 3 It shows the traditional tree planting method in saline-alkali soil in the Shigatse area of Tibet (Nanmulin County).
[0134] (2) Test procedure
[0135] (a) Prepare the solid mixed bacterial agent in the same manner as in Example 1, and combine the above-mentioned Bacillus megaterium, Pseudomonas stutzeri, Lactobacillus delbrueckii, Rhizobium radiata, Alcaligenes faecalis, Candida tropicalis The solid bact...
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