Compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil

A compound microbial inoculant and compound microbial technology, which is applied in the field of soil environment remediation, can solve the problems of high operation and maintenance costs, high consumption of water resources, large maintenance workload, etc., achieving a wide range of applications, improving soil fertility, and a simple process. easy-to-control effects

Active Publication Date: 2016-08-10
陈五岭
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although these technical measures have played a certain role in the process of improving saline-alkali land, they still consume too much water resources in large-scale app

Method used

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  • Compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil
  • Compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil
  • Compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0064] (1) Preparation of bacterial suspension:

[0065] (1-i) Firstly, the five microbial strains of Bacillus megaterium, Pseudomonas stutzeri, Lactobacillus delbrueckii, Rhizobium radiata, Alcaligenes faecalis, and Candida tropicalis were respectively cultured in test tubes and shaken flasks. Cultivation and seed tank cultivation, and then accessing the fermenter liquid at a ratio of 5% to 10% by volume for cultivation, the cultivation temperature of each level of cultivation is 25°C to 35°C (such as 25, 30 or 35°C), and the cultivation time is 24 hours to 48 hours (such as 24, 30, 36, 42 or 48 hours), thereby respectively obtaining the respective viable counts of the five microbial strains greater than or equal to 10 8 / mL (eg 1X10 8 / g, 5X10 8 / g, 1X10 9 / g or 5X10 9 / g) of the first bacterial suspension; and

[0066] (1-ii) Carry out step-by-step liquid static anaerobic expansion culture of Lactobacillus delbrueckii, the culture temperature of each level of culture i...

Embodiment 1

[0109] Example 1 Field experiment on 500 mu of saline-alkali land in Yingnan Village, Dali County, Weibei area

[0110] (1) Test site

[0111] The landform of the test is a depression outside the embankment of the Yellow River, which has not been cultivated for eight years, with reeds growing on it, and thick salt (white) on the surface. The soil types are mainly fluvo-aquic soil and saline soil, the soil salt content is more than 1%, the chemical type is mainly chloride, and a small part is sulfate and silicate. Soil anion type is Cl - 、HCO 3- , SO 4 2- , cation type Na + , Ca 2+ 、K + . Soil organic matter is low, stickiness is high, and crops are not resistant to growth (see figure 1 , which shows the untreated salinized soil in Weibei region).

[0112] (2) Test procedure

[0113] (a) Bacillus megaterium, Pseudomonas stutzeri, Rhizobium radiata, Alcaligenes faecalis, and Candida tropicalis were respectively cultured in test tubes, shake flasks, and seed tanks in s...

Embodiment 2

[0131] Example 2 Microbiological remediation test of 100 mu of saline-alkali soil in Nanmulin County, Shigatse, Tibet

[0132] (1) Test site

[0133] The Shigatse region of Tibet is located on the Tibetan plateau at an altitude of 4,100 meters. The annual precipitation is only 260 millimeters, the evaporation is as high as 2,200 millimeters, and the annual frost-free period is only 120 days. %), the top 50cm of soil is sandy soil with extremely poor water retention, high salinity, and about 50cm of permafrost below the surface for plant roots to grow downward. image 3 It shows the traditional tree planting method in saline-alkali soil in the Shigatse area of ​​Tibet (Nanmulin County).

[0134] (2) Test procedure

[0135] (a) Prepare the solid mixed bacterial agent in the same manner as in Example 1, and combine the above-mentioned Bacillus megaterium, Pseudomonas stutzeri, Lactobacillus delbrueckii, Rhizobium radiata, Alcaligenes faecalis, Candida tropicalis The solid bact...

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Abstract

The invention relates to compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil .In particular, the compound microbial agents comprises the bacillus megaterium microbial agent, the pseudomonas stutzeri microbial agent, the rhizobium radiobacter microbial agent, the candida tropicalis microbial agent and the lactobacillus delbrueckii microbial agent .The invention further comprises the bacterial manure containing the microbial agents, the preparation methods thereof and application thereof to restoration of the saline alkali soil .By means of the compound microbial agents, the bacterial manure, the preparation methods thereof and application thereof to restoration of the saline alkali soil, raw materials which pollute the environment easily or need to be disposed such as straw, excrements of livestocks, household garbage and sludge can be utilized sufficiently, in addition, the compound microbial bacterial manure capable of restoring the saline alkali soil can be prepared, thus the immune function and stress resistance of plants can be improved, the land salinization degree can be improved, and the purpose of achieving second ploughing of salinized soil is achieved.

Description

technical field [0001] The invention relates to a bioremediation process for obtaining safe and long-term bioremediation of polluted and damaged soil by utilizing the functions of selected and bred composite microorganisms to degrade and transform environmental pollution and damage factors, and belongs to the technical field of soil environment remediation. technical background [0002] The formation of saline-alkali land is due to natural or man-made reasons, which increase the underground water level, increase the salinity, dry climate, and increase evaporation, resulting in enhanced salinization or alkalization of the soil surface, and surface salinity or alkalization. aggravated phenomenon. The essence of its formation is mainly the redistribution of various soluble salts in the horizontal and vertical directions on the ground, so that the salt gradually accumulates on the soil surface in the salt-gathering area. [0003] The problems of soil salinization and secondary ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05G1/00C05F17/00B09C1/10B09C1/00C12R1/11C12R1/38C12R1/41C12R1/05C12R1/225C12R1/74
CPCB09C1/00B09C1/105B09C2101/00C05C9/00C05F17/00C12N1/16C12N1/20C05F3/00C05F11/08C05F7/00Y02W30/40
Inventor 陈五岭杜茂林
Owner 陈五岭
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