The invention discloses hair follicle curing shampoo with hair regeneration factors and a preparation method thereof. The hair follicle curing shampoo is prepared from the following raw materials in parts by weight: 0.5 to 1.5 parts of an EGF (Epidermal Growth Factor), 35 to 50 parts of ginger juice, 6 to 18 parts of traditional Chinese medicine extracting solution, 4 to 11 parts of caffeine, 3 to7 parts of trehalose, 2 to 7 parts of vitamin E, 4 to 10 parts of mint essential oil, 3 to 8 parts of rosemary essential oil, 2 to 6 parts of laurinol polyether ammonium sulfate, 1 to 5 parts of disodium cocoyl glutamate, 2 to 7 parts of sodium cocoyl amino-propionate, 3 to 8 parts of cocamidopropyl hydroxyl sulphobetaine, 0.5 to 1.5 parts of lauryl glucoside, 0.4 to 1.8 parts of potassium sorbate, 0.5 to 2.0 parts of citric acid and 1.5 to 4.5 parts of hexadecyl trimethyl ammonium chloride. The EGF, the ginger juice, the traditional Chinese medicine extracting solution, the caffeine, the trehalose, the vitamin E, the mint essential oil, the rosemary essential oil, the laurinol polyether ammonium sulfate, the disodium cocoyl glutamate, the sodium cocoyl amino-propionate, the cocamidopropyl hydroxyl sulphobetaine, the lauryl glucoside, the potassium sorbate, the citric acid and the hexadecyl trimethyl ammonium chloride can be used as the raw materials of the hair follicle caring shampoo through scientific proportioning, and the hair follicle caring shampoo is capable of curing scalp, moistening hair follicles and growing dense hairs while the hairs can be effectively cleaned.