The invention discloses an extraction method and application of high-activity collagen, and belongs to the fields of biotechnology and cosmetics. The collagen is extracted by adopting a combined extraction method, so that the extraction time is shortened, the extraction rate is improved, the purity is improved, the environmental pollution is avoided, and the natural biological character of the collagen is maintained to the maximum degree. On that basis, the invention further provides a fishskin collagen anti-wrinkle essence. The adjustment of skin cutin can be helped, the cutin is smooth, theskin is whitened in an orienteering manner, the activity of face cells is stimulated, darkness is decomposed in multiple layers, the essence is antioxidant, the skin color is balanced, and damaged cells caused by ultraviolet rays or other cosmetics are repaired.