Extraction method and application of high-activity collagen

An extraction method and collagen technology, applied in the fields of biotechnology and cosmetics, can solve problems such as shortening the extraction time, and achieve the effects of shortening the extraction time, no skin irritation, and improving the extraction rate

Inactive Publication Date: 2018-04-20
大美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to overcome the deficiency of a single extraction method and improve the extraction rate of collagen, the present invention adopts a combined extraction method to extract collagen, which shortens the extraction time, improves the extraction rate, improves the purity, has no pollution to the environment and maintains the natural nature of collagen to the greatest extent. biological traits

Method used

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  • Extraction method and application of high-activity collagen
  • Extraction method and application of high-activity collagen
  • Extraction method and application of high-activity collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of baby fish skin collagen

[0035] Step 1: Tissue fragmentation: After thawing the adult (weight 2-3 kg) baby fish (artificially farmed) skin, add water so that the weight ratio of the skin to water is 10:1. The mixture is crushed and ground. Ultrasound is applied at the same time of crushing and grinding, the intensity of ultrasonic is controlled at 800 watts / liter, and the processing time of ultrasonic is 30 minutes to obtain slurry;

[0036] The second step is to remove salt-soluble impurities: mix the slurry with sodium chloride solution (mass concentration 5%), the weight ratio of slurry to sodium chloride solution is 1:5, stir well, soak for 15h, 1500rpm, centrifuge for 10min, Remove the supernatant to obtain the first precipitate;

[0037] Step 3, remove acid-soluble impurities: mix the first precipitate with citric acid solution (mass concentration is 6%), the weight ratio of the first precipitate to acid solution is 1:5, soak for 6h, 1500rpm, ...

Embodiment 2

[0042] Example 2: Preparation of Tilapia Skin Collagen

[0043] Step 1: Tissue fragmentation: After thawing adult tilapia skins (weight 2-3 kg), add water to make the weight ratio of skin to water 10: (0.2-1), crush and grind the mixture , The ultrasonic effect is applied while crushing and grinding, the ultrasonic intensity is controlled at 900 watts / liter, the ultrasonic processing time is 40 minutes, and the slurry is obtained;

[0044] The second step is to remove salt-soluble impurities: mix the slurry with sodium chloride solution (mass concentration 5%), the weight ratio of slurry to sodium chloride solution is 1:5, stir well, soak for 15h, 1500rpm, centrifuge for 10min, Remove the supernatant to obtain the first precipitate;

[0045] Step 3, remove acid-soluble impurities: mix the first precipitate with citric acid solution (mass concentration is 6%), the weight ratio of the first precipitate to acid solution is 1:5, soak for 6h, 1500rpm, centrifuge for 10min, remove the sup...

Embodiment 3

[0050] Example 3: Preparation of cod fish skin collagen

[0051] Step 1: Tissue fragmentation: After thawing adult (2-3 kg) cod skin, add water to make the weight ratio of skin to water 10:0.2-1, crush and grind the mixture, and then Ultrasound is applied at the same time of grinding, the intensity of ultrasonic is controlled at 750 watts / liter, and the processing time of ultrasonic is 60 minutes to obtain slurry;

[0052] The second step is to remove salt-soluble impurities: mix the slurry with sodium chloride solution (mass concentration 5%), the weight ratio of slurry to sodium chloride solution is 1:5, stir well, soak for 15h, 1500rpm, centrifuge for 10min, Remove the supernatant to obtain the first precipitate;

[0053] Step 3, remove acid-soluble impurities: mix the first precipitate with citric acid solution (mass concentration is 6%), the weight ratio of the first precipitate to acid solution is 1:5, soak for 6h, 1500rpm, centrifuge for 10min, remove the supernatant , Get t...

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Abstract

The invention discloses an extraction method and application of high-activity collagen, and belongs to the fields of biotechnology and cosmetics. The collagen is extracted by adopting a combined extraction method, so that the extraction time is shortened, the extraction rate is improved, the purity is improved, the environmental pollution is avoided, and the natural biological character of the collagen is maintained to the maximum degree. On that basis, the invention further provides a fishskin collagen anti-wrinkle essence. The adjustment of skin cutin can be helped, the cutin is smooth, theskin is whitened in an orienteering manner, the activity of face cells is stimulated, darkness is decomposed in multiple layers, the essence is antioxidant, the skin color is balanced, and damaged cells caused by ultraviolet rays or other cosmetics are repaired.

Description

Technical field [0001] The invention relates to a method for extracting highly active collagen and its application, and belongs to the fields of biotechnology and cosmetics. Background technique [0002] Collagen is a biopolymer, the main component of animal connective tissue, and it is also the most abundant and widely distributed functional protein in mammals, accounting for 25%-30% of the total protein, and some organisms can even reach more than 80%. . [0003] Collagen has a wide range of applications and can be applied to basic research in biotechnology, such as cell primary culture. The addition of collagen can greatly increase the success rate of culture and cell yield; it can be used for medical external application, and it can be made into dressings to promote wound healing. Repair damaged body tissues; can be used in the medical industry to be used with injection drugs to lubricate joints when treating sports injuries; can be used in health care products as oral liquids...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/65A61K8/73A61K8/365A61K8/34A61K8/60A61Q19/08A61Q19/02A61Q19/00A61K38/39A61P17/02A61P17/18A61P19/02A61L15/32A61L15/40C12P21/06C07K14/78C07K1/30A23L33/17A61K31/728A61K31/194A61K31/05A61K31/7034
CPCA23L33/17A23V2002/00A61K8/347A61K8/365A61K8/602A61K8/65A61K8/735A61K31/05A61K31/194A61K31/7034A61K31/728A61K38/39A61L15/325A61L15/40A61L2300/252A61L2300/30A61Q19/00A61Q19/004A61Q19/02A61Q19/08C07K14/78C12P21/06A61K2300/00A23V2200/318Y02P20/54
Inventor 周萱
Owner 大美生物科技有限公司
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