The invention relates to a plant moisturizing toner for people staying up all night and a preparation method thereof. The plant moisturizing toner comprises water, butanediol, diglycerin, sodium hyaluronate, sodium polyacrylate, xanthan gum, tremella polysaccharide, tremella extract, vitamin C magnesium phosphate, saussurea involucrata extract, edelweiss extract, reed extract, licorice root extract, angelica root extract, salvia miltiorrhiza extract, dogwood fruit extract, boxthorn root extract, p-hydroxyacetophenone and 1,2-hexanediol. The preparation method includes the steps of mixing and heating the sodium polyacrylate, the xanthan gum, the water, the butanediol, the diglycerin, the sodium hyaluronate and the p-hydroxyacetophenone; adding the tremella polysaccharides, the tremella extract, the vitamin C magnesium phosphate, the saussurea involucrata extract, the edelweiss extract, the reed extract, the licorice root extract, the angelica root extract, the salvia miltiorrhiza extract, the dogwood fruit extract, the boxthorn root extract and the 1,2-hexanediol after cooling down, and performing stirring; performing cooling and discharging. The plant moisturizing toner has a goodskin nourishing effect.