The invention belongs to the technical field of preparation of display screens, in particular to a laser projection display screen with a light absorption layer and a preparing method and applicationthereof. The laser projection display screen comprises a protection layer, a blue fluorescent material layer, a green fluorescent material layer, a red fluorescent material layer/an up-conversion luminescent material layer and a substrate, wherein the protection layer, the blue fluorescent material layer, the green fluorescent material layer, the red fluorescent material layer/the upper conversionluminescent material layer and the substrate are stacked in sequence. The fluorescent material layers are separated by light absorption separation layers, so that excitation on the green fluorescentmaterial layer and the red fluorescent material layer by high-energy laser is avoided, and lowering of the colour purity is inhibited. The laser projection display screen is made of an ultra-flat transparent material, and can avoid the diffuse reflection phenomenon. Adopted organic or polymer green and blue fluorescent materials have anisotropy, and can avoid specular reflection. An up-conversionluminescent material can obtain saturated light, and has high luminescent efficiency and good stability. When laser light is used as a light source, the distinguishability of the laser projection display screen can reach up to 10 k. The preparing method of the laser projection display screen is simple, and the laser projection display screen is low in cost, long in service life and light, can be folded and unfolded, is waterproof, does not need to be driven by power, and can be applied to multiple fields.