The present invention relates to a seaweed fiber care type mascara and a preparation method thereof. The seaweed fiber care type mascara is characterized by comprising, by weight, 0.5-2.5% of seaweed fibers, 0.5-2.5% of algina, 0.5-2.0% of symphytum officinale (allantoin), 1.0-2.0% of natural Vitamin E, 0.2-1.0% of olive oil, 10-20% of beeswax, 10-25% of white cordyceps sinensis, 5-10% of radix polygoni multiflori, 0.5-2% of ginseng, 1.0-5.0% of tremella, 2.0-5.0% of carbon powder, 0.5-1.0% of lysozyme, and the balance of deionized water. Compared with the mascara in the prior art, the mascara of the present invention has the following advantages that: the mascara of the present invention is the care type mascara developed by adopting the pure nature marine algae and plant as the raw material, generation of collagen in the eyelid skin can be stimulated with various natural plant essences so as to make the eyelid skin closely hold the root of the eyelash, significantly reduce eyelash shedding, supplement the nutrition, improve the eyelash growth environment, and make the eyelash thick, slender and shiny, the formula of the present invention is the pure nature formula, and characteristics of mildness and no irritation on the eye sensitive part, nourishing and protection two-in-one property and easy remover are provided.