The invention relates to an anti-hair loss and hair-strengthening nutrient solution and a preparation method thereof. The anti-hair loss and hair-strengthening nutrient solution is prepared from the following raw material components in parts by weight: 80 to 90 parts of compound plant extract, 5 to 9 parts of a hair conditioner, 3.5 to 10 parts of a humectant and 0.2 to 0.4 part of a thickening agent, wherein the composite plant extract is a mixture of water, a cacumen biotae extract, a radix polygoni multiflori extract, a ginger root extract, a folium mori extract, a black mulberry fruit extract, a sesame seed extract and a ginseng root extract, and the hair conditioner is a mixture of water, propylene glycol, glycerol, 1, 2-hexanediol, diaminopyrimidine oxide, polyglycerol-6 myristate, caprylic / capric triglyceride, pyrrolidinyl diaminopyrimidine oxide, lecithin, hydroxylated lecithin and cholesterin. The anti-hair loss and hair-strengthening nutrient solution has good effects of strengthening and toughening hair roots, preventing hair loss and promoting hair growth.