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Composite microbial bacterial agent and bacterial fertilizer and its preparation method and application in repairing saline-alkali soil

A compound microbial inoculant and compound microbial technology, applied in the field of soil environmental remediation, can solve the problems of high operation and maintenance costs, heavy maintenance workload, and high water consumption, achieving a wide range of applications, simple and easy-to-control processes, and improved The effect of soil fertility

Active Publication Date: 2019-08-09
陈五岭
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although these technical measures have played a certain role in the process of improving saline-alkali land, they still consume too much water resources in large-scale applications, the project scale is large, land occupation is large, investment is large, maintenance workload is large, and operation and maintenance costs are high. Higher questions

Method used

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  • Composite microbial bacterial agent and bacterial fertilizer and its preparation method and application in repairing saline-alkali soil
  • Composite microbial bacterial agent and bacterial fertilizer and its preparation method and application in repairing saline-alkali soil
  • Composite microbial bacterial agent and bacterial fertilizer and its preparation method and application in repairing saline-alkali soil

Examples

Experimental program
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Effect test

preparation example Construction

[0065] (1) Preparation of bacterial suspension:

[0066] (1-i) First, the five microbial strains of Bacillus megaterium, Pseudomonas stutzeri, Lactobacillus delbrueckii, Rhizobia, Alcaligenes faecalis, and Candida tropicalis were sequentially cultured in test tubes, shake flasks Cultivation and seed tank cultivation, and then access the fermenter liquid in a proportion of 5 to 10 vol% for cultivation, the cultivation temperature of each level of cultivation is 25°C to 35°C (for example, 25, 30 or 35°C), and the cultivation time is 24 hours to 48 hours (for example 24, 30, 36, 42 or 48 hours), thereby obtaining the respective viable counts of the five microbial strains greater than or equal to 10 8 / mL (e.g. 1X10 8 / g, 5X10 8 / g, 1X10 9 / g or 5X10 9 / g) of the first bacterial suspension; and

[0067] (1-ii) Carry out step-by-step liquid static anaerobic expansion culture of Lactobacillus delbrueckii, the culture temperature of each level of culture is 35°C to 40°C (for exa...

Embodiment 1

[0110] Example 1 Field test of 500 mu of saline-alkali land in Yingnan Village, Dali County, Weibei Region

[0111] (1) Test site

[0112] The test landform is a depression outside the embankment of the Yellow River. The cultivation has been stopped for eight years, and there are reeds growing on the surface. The surface has thicker salt (white). The soil types are mainly fluvo-aquic soil and saline soil, the soil salt content is more than 1%, the chemical type is mainly chloride, and a small part is sulfate and silicate. Soil anion type is Cl - , HCO 3- , SO 4 2- , cation type Na + , Ca 2+ , K + . Soil organic matter is low, stickiness is high, and crops are not resistant to growth (see figure 1 , which shows untreated saline-alkali soils in the Weibei region).

[0113] (2) Test steps

[0114] (a) Bacillus megaterium, Pseudomonas stutzeri, Rhizobia radiata, Alcaligenes faecalis, and Candida tropicalis are respectively subjected to test tube culture, shake flask cul...

Embodiment 2

[0132] Example 2 Microbiological remediation test of 100 mu of saline-alkali soil in Nanmulin County, Shigatse, Tibet

[0133] (1) Test site

[0134] The Shigatse region of Tibet is located on the Tibetan plateau at an altitude of 4,100 meters. The annual precipitation is only 260 millimeters, the evaporation is as high as 2,200 millimeters, and the annual frost-free period is only 120 days. %), the top 50cm of soil is sandy soil with extremely poor water retention, high salinity, and about 50cm of permafrost below the surface for plant roots to grow downward. image 3 It shows the traditional tree planting method in saline-alkali soil in the Shigatse area of ​​Tibet (Nanmulin County).

[0135] (2) Test procedure

[0136] (a) Prepare the solid mixed bacterial agent in the same manner as in Example 1, and combine the above-mentioned Bacillus megaterium, Pseudomonas stutzeri, Lactobacillus delbrueckii, Rhizobium radiata, Alcaligenes faecalis, Candida tropicalis The solid bact...

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Abstract

The invention relates to compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil .In particular, the compound microbial agents comprises the bacillus megaterium microbial agent, the pseudomonas stutzeri microbial agent, the rhizobium radiobacter microbial agent, the candida tropicalis microbial agent and the lactobacillus delbrueckii microbial agent .The invention further comprises the bacterial manure containing the microbial agents, the preparation methods thereof and application thereof to restoration of the saline alkali soil .By means of the compound microbial agents, the bacterial manure, the preparation methods thereof and application thereof to restoration of the saline alkali soil, raw materials which pollute the environment easily or need to be disposed such as straw, excrements of livestocks, household garbage and sludge can be utilized sufficiently, in addition, the compound microbial bacterial manure capable of restoring the saline alkali soil can be prepared, thus the immune function and stress resistance of plants can be improved, the land salinization degree can be improved, and the purpose of achieving second ploughing of salinized soil is achieved.

Description

technical field [0001] The invention relates to a bioremediation process for obtaining safe and long-term bioremediation of polluted and damaged soil by utilizing the functions of selected and bred composite microorganisms to degrade and transform environmental pollution and damage factors, and belongs to the technical field of soil environment remediation. [0002] technical background [0003] The formation of saline-alkali land is due to natural or man-made reasons, which increase the underground water level, increase the salinity, dry climate, and increase evaporation, resulting in enhanced salinization or alkalization of the soil surface, and surface salinity or alkalization. aggravated phenomenon. The essence of its formation is mainly the redistribution of various soluble salts in the horizontal and vertical directions on the ground, so that the salt gradually accumulates on the soil surface in the salt-gathering area. [0004] The problems of soil salinization and se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C05G1/00C05F17/00B09C1/10B09C1/00C12R1/11C12R1/38C12R1/41C12R1/05C12R1/225C12R1/74
CPCB09C1/00B09C1/105B09C2101/00C05C9/00C05F17/00C12N1/16C12N1/20C05F3/00C05F11/08C05F7/00Y02W30/40
Inventor 陈五岭杜茂林
Owner 陈五岭
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