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Biocontrol strain SFC-3 for controlling nematode disease and application thereof

A technology of SFC-3 and biocontrol strains, applied in the fields of application, nematicides, biocides, etc., can solve the problems of high cost of use, unstable control effect, etc., and achieve the effects of promoting growth, reducing dependence, and not polluting the environment

Inactive Publication Date: 2019-01-15
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, in order to solve the defects of the prior art, the purpose of the present invention is to provide a kind of biocontrol bacterial strain SFC-3 and its application for the prevention and treatment of nematode disease, which can effectively solve the existing prevention and treatment of root-knot nematode disease. Unstable efficiency and high cost of use

Method used

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Embodiment Construction

[0015] The specific implementation manners of the present invention will be described in further detail below in combination with actual conditions.

[0016] The source of the biocontrol strain SFC-3 of the present invention is obtained by separating and purifying soil samples from vegetable greenhouses in Hua County, Anyang City, Henan Province.

[0017] Isolation method of biocontrol strain SFC-3:

[0018] 1) Soil sample source: Soil samples were collected from the land with severe root-knot nematode disease in vegetable greenhouses in Hua County, Anyang City, Henan Province;

[0019] 2) Weigh 10g of soil sample into a conical flask filled with 90mL of sterile water, place it on a shaker for 25-35 minutes, and obtain a dilution factor of 10 -1 Soil dilution solution, take 1mL 10 -1 The soil dilution was added to a test tube containing 9 mL of sterile water to obtain a dilution factor of 10 -2 Take 300 μL of the diluted soil solution and spread it on the Martin's medium, a...

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Abstract

The invention relates to a biocontrol strain SFC-3 for controlling nematode disease and application thereof, which can effectively solve that problem of unstable control effect and high use cost of existing biocontrol strain for controlling root-knot nematode diseases, and is classify and named as Trichoderma asperellum (CGMCC NO. 16097), which is a biocontrol strain SFC-3. At the concentration of1 * 109 CFU / mL, the egg hatching and the second instar larvae of root-knot nematode are inhibited. The strain can remarkably reduce the invasion of nematodes, and the control effect on root-knot nematode diseases is 78.51%. The strain can be used for irrigating roots when vegetable seedlings are transplanted, and has the growth promoting effect.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a biocontrol bacterial strain SFC-3 for preventing nematode disease and application thereof. Background technique [0002] Vegetable root-knot nematode is a soil-borne disease that seriously affects vegetable growth. Roots can infect root cells, destroy the root conduction tissue, and lose the ability to absorb nutrients and water, leading to malnutrition and premature aging of crops, and even the whole plant withers and dies. So far, more than 80 kinds of root-knot nematodes have been reported internationally. There are 39 effective species recorded in my country, which can infect 114 families and more than 3000 kinds of plants including monocotyledonous plants, dicotyledonous plants and herbaceous plants. Root-knot nematodes are mainly caused by the following four species: root-knot nematode southern, root-knot nematode northern, root-knot nematode java and root-knot nematode ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02A01N63/04A01P5/00C12R1/885
CPCA01N63/30C12N1/02C12N1/14C12N1/145C12R2001/885
Inventor 陈国参赵俊杰王继雯李冠杰甄静岳丹丹巩涛杨文玲杜志敏孙晨阳杨金星
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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