105 results about "Tapeworm egg" patented technology

The invention provides an artificial breeding production method of trichogramma japonicum. The method is high in production efficiency, short in breeding cycle and beneficial to large-scale production of the richogramma japonicum. The method comprises the following steps that 1, sticky eggs are produced; 2, the sticky eggs are dissolved into single particles from an oviposition medium with a neutral protease solution with the concentration being 0.2%-0.5% (g/ml); 3, the sticky eggs treated in the step 2 are used for breeding the trichogramma japonicum. According to the method, the trichogramma japonicum bred artificially is used for preventing and treating agricultural insects, and the agricultural insects mainly comprise rice-stem borer, tryporyza incertulas and rice leaf folder. Compared with the prior art, the method has the advantages that armyworms are simple in source and easy and convenient to breed; the method is high in production efficiency and short in breeding period, large-scale breeding is convenient, and large-scale production of the trichogramma japonicum is promoted.

The invention discloses a composition for attracting hermetia illucens for concentrated oviposition and an application of the composition, and belongs to the technical field of insect breeding. The composition includes an attracting odor substance arranged on an oviposition substrate, and the attracting odor substance includes one or more selected from the group consisting of methyl acrylate, lactic acid, 3-methylindole, n-heptanoic acid and dimethyl disulfide, the odor substance having the attracting effect and oviposition substrate color having the attracting effect are combined to attract the hermetia illucens to crack channels of corrugated paper for oviposition, insect eggs are mainly concentrated in the corrugated paper, so that the oviposition is more concentrated, and the eggs areeasier to collect. The composition provided by the invention has the multiple combined effects of the odor substance, the oviposition substrate color and the crack channels, and can enhance the oviposition attracting effect on the hermetia illucens, thereby improving the concentrated oviposition and egg collection efficiency of the hermetia illucens.

The invention discloses a Trichinella Spiralis larva ES antigen gene vaccine and a preparation method thereof. Trichinella Spiralis larva 43ku and 45ku ES protein genes are respectively inserted to a pVAXI eukaryotic expression vector to obtain recombinant plasmids, and the recombinant plasmids are mixed according to a ratio of 1:1 to prepare a combined recombined DNA vaccine for preventing Trichinella Spiralis infection and improving the worm reduction rate of bodies. After the injection immunization of hindlimb muscles of mice for three weeks, 2000 worms are killed, the worm reduction rate can reach 76.65%, and the quantity of eggs at the tongue tissues of the mice is obviously reduced.

The invention discloses a method of fast separating Japan schistosome unfledged spawn, which employs technique of combining repeat sieving and pancreatin processing and completes in two hours. Purity of the obtained spawn is above 99%, structure of the spawn is integrated and good biological activity is remained, which can satisfy basic need of antigen extraction and analysis. Comparing to exist technique, yield of the spawn is largely increased.

The invention discloses a solid mixture separation device, and particularly discloses an egg and adult insect scale powder separator. The solid mixture separation device comprises a separation chamber arranged vertically, a feeding device, an air suction device and a receiving device, wherein a feeding port and an air suction port are formed at the upper end of the separation chamber; an outlet is formed at the lower end of the separation chamber; the feeding device is used for feeding a solid mixture to the separation chamber; the air suction device is connected with the air suction port; and the receiving device is positioned at the lower part of the outlet. The solid mixture separation device is applicable to the separation of an egg and adult insect scale powder mixture; eggs and powder that are in different weights are automatically separated through wind power, so that the collected eggs are further purified; the solid mixture separation device ensures that the clean eggs can be collected; a raising room is kept clean; and the purposes of protecting the environment and human health are achieved.

The present invention provides special primers for rapidly identifying anoplocephalidae tapeworms parasitizing on Equine, wherein the upstream primer is 5'-GCAGAGTTTTGCTGATTTTATGAAG-3', and the downstream primer is 5'-CCACTATACTCAGTATTAAATCCACTAACAAG-3'. The present invention further provides a method for rapidly identifying anoplocephalidae tapeworms parasitizing on Equine, wherein the method comprises: adopting the primers to carry out PCR amplification on NAD1 of anoplocephalidae tapeworm, sequencing, comparing the sequencing result with sequences in SEQIDNo.1 and SEQIDNo.2, and judging. With the method, the defects that the general morphology observation can not distinguish gravid proglottids and the microscopic observation can not distinguish eggs are solved. The PCR method of the present invention can be used for molecule identification of two anoplocephalidae tapeworms, and can further be used for epidemiological investigation of the Equine anoplocephalidae tapeworm.

The invention discloses an arma chinensis cold storage diapause method. The method comprises the following steps that (1) arma chinensis armyworm eggs are selected as cold storage diapause objects; and (2) the arma chinensis armyworm eggs selected in the step (1) are placed in a cold storage diapause environment, wherein the cold storage diapause temperature is 2-6 DEG C, and the cold storage diapause time is 8-12 days. Aiming at arma chinensis which is an excellent predatory natural enemy insect, the control research of regulating and controlling the arma chinensis armyworm egg incubation time by utilizing factors such as cold storage temperature and time is carried out, the research finds that the incubation rate of the arma chinensis armyworm eggs still reaches 50% or above after the arma chinensis armyworm eggs stand for 6-14 days in the cold storage diapause environment with the temperature of 0-8 DEG C, and large-scale cultivation requirements can be basically met.

The invention discloses an artificial breeding method for increasing the biomass of hermetia illucens larvae. The method comprises the following steps of inoculating hermetia illucens eggs into an incubation material of bran corn flour and special feed, incubating, and continuously culturing in a system to obtain larvae; and then transferring the larvae into a larva material, added with flavonoidcompounds, of chicken manure and the bran corn flour, and continuously culturing until prepupae appear and harvesting the larvae. According to the method, the flavonoid compounds are added in the hermetia illucens breeding process, more nutrient substances in the materials are converted into self substances, and therefore hermetia illucens larva biomass accumulation can be remarkably increased. The method is simple, effective and easy to operate, the problem of conversion rate limitation in the current hermetia illucens composting technology is solved, and favorable conditions are created forpopularization and application of the hermetia illucens technology.

The invention belongs to the technical field of aquaculture, and particularly discloses a method for producing sterile fairy shrimps and application of the sterile fairy shrimps. The method comprises the following steps: (1) bleaching fairy shrimp eggs with a bleaching agent; (2) filtering the eggs bleached in the step (1) by using a filter screen, and washing by using sterile water to remove the bleaching agent on the surfaces of egg shells; and (3) culturing the eggs obtained in the step (2) in saline water, hatching the eggs, and then separating the hatched fairy shrimps from egg shells to obtain the sterile fairy shrimps. The effective sterile fairy shrimp production method is established, safe and reliable feed is provided for sterile fishes, hope is provided for establishing an adult sterile fish model, and convenient materials are provided for studying interaction and multiple biology between microorganisms and fishes by using the sterile fishes; the method is of great significance to further research on the internal mechanism of the relationship between microorganisms and vertebrate hosts thereof.

The invention provides an intelligent breeder selection hatching culture control device for comprehensively simulating natural environment conditions of locust egg laying and egg hatching based on locust ethology. Optimal environment conditions are simulated for locust egg laying and egg hatching, a convenient and rapid operation method is provided for feeders, locust egg hatching conditions are automatically monitored by technologies such as intelligent temperature control, automatic control and image recognition, and data such as the total number of related eggs, the excellence rate of the eggs, the sizes of individual eggs, egg duration and hatching rate are measured. Besides, egg excellence conditions can be intelligently monitored, summer locust, autumn locust and egg overwintering conditions are intelligently selected, different environment conditions and monitoring data in three modes are provided, the egg laying amount and the hatching amount of locusts in summer and autumn areeffectively increased, and value output and production efficiency are greatly improved.

The invention relates to the technical field of plant protection and insect breeding, in particular to a method for feeding orius sauteri(poppius) by adopting oriental armyworm eggs as feed. The oriental armyworm eggs utilized by the method can meet the nutritional requirements of the orius sauteri(poppius) during a whole growth period, and improve existing problems brought by feeding the orius sauteri(poppius) by utilizing flour mites, aphids and the like as feed; and by utilizing the characteristics that a large-scale feeding technique utilizing oriental armyworms has matured, the oriental armyworm eggs are easy to obtain and store, the requirement on a production environment is low, and the like, the foundation in feed is provided for large-scale feeding of the orius sauteri(poppius), and the feeding effect is good.

The invention discloses a method for separating living eggs of meloidogyne incognita. The method comprises the following steps of stripping meloidogyne incognita oocysts attached to the surface of a root system to be subjected to static incubation, NaOCl dissociation, cleaning, oscillation, centrifugation and the like, so that pure meloidogyne incognita eggs with a dispersed single population canbe separated, meanwhile, the activity of the eggs is ensured, and value for popularization and application is achieved.

The invention discloses a hermetia illucens embryo microinjection method. The method comprises the following steps: (1) collecting eggs newly laid by mated hermetia illucens female individuals; (2) neatly placing the eggs obtained in the step 1) on a glass slide; (3) placing the glass slide on a microinjection table for microinjection, controlling the temperature of a capillary glass tube which isused to be 60-65 DEG C during needle pulling, controlling an injection angle to be an included angle between a capillary glass needle and an egg to be 20-45 degrees, controlling the injection position to be at the lower part of the embryo, and controlling the time from egg collection to injection completion to be within 0.5-2 hours; and (4) performing putting into an incubator at 24-26 DEG C, controlling the humidity to be 50-80%, and continuously performing culturing in the dark. A hermetia illucens egg collection technology and a micromanipulation technology are combined, so that the hermetia illucens embryo microinjection platform is successfully established. An egg collecting and placing method is simple, time-saving and labor-saving, and the survival rate of embryos can be greatly increased by strict implementing according to injection conditions.

The invention discloses siRNA of schistosoma japonicum SjELAV-like 1 genes. The siRNA has nucleotide sequences shown as SEQ ID NO.3 and SEQ ID NO.4. The invention further discloses the application ofthe siRNA of schistosoma japonicum SjELAV-like 1 genes. The siRNA of schistosoma japonicum SjELAV-like 1 genes disclosed by the invention is capable of obviously inhibiting transcription of the schistosoma japonicum SjELAV-like 1 genes and obviously reducing the number of liver loaded eggs and egg hatching rate of schistosomiasis infected mice, causes certain damages to the tegumental structure ofschistosoma and causes morphological abnormality of germ cells, and is applicable to preparation of drugs for treating schistosomiasis.