Method for preparing bio-organic fertilizer by utilizing chicken manure fermentation
A bio-organic fertilizer and chicken manure technology, which is applied in the preparation of organic fertilizers, the treatment of bio-organic parts, organic fertilizers, etc., can solve the problems of high energy consumption, high equipment requirements, and high nutrient loss, and achieve less energy consumption and shorter The effect of fermentation time and operation is simple and easy
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Embodiment 1
[0025] 1. The preparation method of microbial liquid bacterial agent is as follows:
[0026] (1) Mix and inoculate the strains of Enterococcus faecium, Pediococcus pentosacea and Lactobacillus salivarius preserved on the slant into MRS liquid medium, and culture at 37°C for 20 hours, so that the effective number of viable bacteria in the final fermentation liquid is ≥10 10 CFU / mL, as a lactic acid bacteria solution;
[0027] (2) Inoculate the S. thermophila strain preserved on the slant into PDA liquid medium, and culture it on a shaker at 45°C and 150rpm for 18h, so that the effective number of viable bacteria in the final fermentation liquid is ≥10 9 CFU / mL.
[0028] (3) Inoculate the Bacillus subtilis strain preserved on the slant into LB liquid medium, and culture it on a shaker at 28°C and 150rpm for 24 hours, so that the effective number of viable bacteria in the final fermentation liquid is ≥10 9 CFU / mL.
[0029] (4) Inoculate the NJ26 strain of thermophilic heterotr...
Embodiment 2
[0041] 1. Preparation method of microbial liquid bacterial agent
[0042] (1) Mix and inoculate the strains of Enterococcus faecium, Pediococcus pentosacea and Lactobacillus salivarius preserved on the slant into MRS liquid medium, and culture at 37°C for 18 hours, so that the number of effective viable bacteria in the final fermentation liquid is ≥10 10 CFU / mL, as a lactic acid bacteria solution;
[0043] (2) Inoculate the S. thermophila strain preserved on the slant into the PDA liquid medium, and cultivate it on a shaker at 45°C and 150rpm for 20 hours, so that the effective number of viable bacteria in the final fermentation broth is ≥10 9 CFU / mL.
[0044] (3) Inoculate the Bacillus subtilis strain preserved on the slant into LB liquid medium, and culture it on a shaker at 28°C and 150rpm for 30h, so that the effective number of viable bacteria in the final fermentation liquid is ≥10 9 CFU / mL.
[0045] (4) Inoculate the thermophilic heterotrophic ammonium oxidizing bact...
Embodiment 3
[0058] 1. The preparation method of microbial liquid bacterial agent is as follows:
[0059] (1) Mix and inoculate the strains of Enterococcus faecium, Pediococcus pentosacea and Lactobacillus salivarius preserved on the slant into MRS liquid medium, and culture at 37°C for 16 hours, so that the number of effective viable bacteria in the final fermentation liquid is ≥10 10 CFU / mL, as a lactic acid bacteria solution;
[0060] (2) Inoculate the S. thermophila strain preserved on the slant into the PDA liquid medium, and culture it on a shaker at 45°C and 150rpm for 16 hours, so that the effective number of viable bacteria in the final fermentation liquid is ≥10 9 CFU / mL.
[0061] (3) Inoculate the Bacillus subtilis strain preserved on the slant into LB liquid medium, and culture it on a shaker at 28°C and 150rpm for 28h, so that the effective number of viable bacteria in the final fermentation liquid is ≥10 9 CFU / mL.
[0062] (4) Inoculate the NJ26 strain of thermophilic hete...
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