12 results about "Adenine nucleoside" patented technology

The invention discloses a method for increasing the content of ergosterol in tremella aurantialba mycelia by using adenosine, and relates to the technical field of biological ferment.The method comprises the following steps that a sterilized liquid culture medium is inoculated with tremella aurantialba strains, and liquid culture is conducted to obtain a liquid culture solution; centrifuging the liquid culture solution to obtain mycelia; the content of ergosterol in the obtained mycelium is greater than 34.6 mg / L; a liquid fermentation culture medium is composed of 200 g / L of potatoes, 20 g / L of glucose, 3 g / L of monopotassium phosphate, 1.5 g / L of magnesium sulfate and 12.0 mg / L of vitamin B1, water is added to make the volume constant to 1000 mL, and the pH value is natural; adding adenosine into the liquid culture medium until the concentration of adenosine is greater than 1.0 g / L; the classification of the inoculated tremella auramtialba strain is named as Naematia auramtialba, and the classification is named as Naematia auramtialba; the preservation number of the strain is CGMCC (China General Microbiological Culture Collection According to the method, the content of ergosterol in the obtained tremella aurantialba mycelia can be increased to 45.8 mg / L and the biomass to 12.5 g / L under the condition that the biomass of the mycelia of the tremella aurantialba No. 2 ZJJE002 (the preservation number is CGMCC NO.40124) in the tremella aurantialba strain is not influenced, and the content of ergosterol is 1.55 times that of a control group.

The invention discloses a synthesis method of 2 '-O-methyladenosine, and belongs to the technical field of nucleoside synthesis and medicinal chemistry. According to the method, xylose which is rich in source is taken as an initial raw material, eight-step reaction including acetalation protection, acetylation, glycosylation, deacetylation, methylation, deacetalation protection, Mitsunobu reaction and secondary deacetylation is performed, reaction conditions of each step are accurately controlled, and finally, a target product is synthesized. The method has the advantages of high methylation reaction regioselectivity, mild reaction conditions, cheap and easily available raw materials and controllable cost, and overcomes the problems of poor methylation reaction regioselectivity, harsh reaction conditions and high cost in the existing synthesis method. The obtained product has important application value in the fields of research and development of antiviral drugs, design of nucleic acid probes and the like.

This invention discloses a machine learning-enhanced fluorescence detection method for silver nanoclusters containing split nucleic acid aptamers, used for the detection of adenosine triphosphate (ATP) in aquatic products, belonging to the field of analytical chemistry. This detection method uses two split nucleic acid aptamers of ATP as templates to synthesize DNA-AgNCs. In the presence of the target ATP, a conformational change is induced, resulting in a decrease in fluorescence intensity. Quantitative analysis of ATP is achieved by detecting this fluorescence change. To further improve the detection effect, machine learning is introduced to preprocess the fluorescence spectrum. A recursive feature elimination algorithm is used to screen characteristic wavelengths, and a multi-dimensional feature set is constructed using maximum fluorescence intensity, peak area, and full width at half maximum (FWHM). The optimal classification model is obtained through cross-validation optimization. This invention combines aptamer recognition, DNA-AgNCs, and machine learning to construct a detection method where fluorescence dynamically decreases with increasing ATP concentration. It has advantages such as low cost, simple operation, and good biocompatibility, effectively improving detection sensitivity and stability, and has promising application prospects in the qualitative and quantitative analysis of ATP in aquatic products.

The application discloses a method and a kit for immunodetection of target substances by using arterial blood, and belongs to the technical field of blood detection. The kit comprises at least: a first component containing luminescent microspheres combined with detection antibody 1 and a luminescent microsphere diluent, wherein the luminescent microsphere diluent contains 2-morpholinoethanesulfonic acid, KCl and NaHCO3; a second component containing biotin-labeled detection antibody 2 and a biotin diluent, wherein the biotin diluent contains dithiothreitol, CaCl2, adenine nucleoside and mouse anti-human hemoglobin antibody; and a third component containing photosensitive microspheres combined with streptomycin. The scheme can be used for immunodetection of target substances by using arterial blood, has excellent functional sensitivity and detection range, is not interfered by other substances in arterial blood, realizes rapid detection of all blood gas indexes and immunological indexes by using one tube of arterial blood, and has important significance for clinical blood detection.

The invention belongs to the technical field of biological catalytic synthesis, and particularly relates to a method for synthesizing beta-nicotinamide mononucleotide (NMN) by enzyme catalysis, which comprises the following steps: by taking D-ribose as a starting substrate, and utilizing a three-step enzyme catalysis reaction system which is synergistically completed by an S-methyl-5-thioribose kinase mutant (SMTK), purine nucleotidase (PNP) and nicotinamide ribose kinase (NRK), synthesizing beta-nicotinamide mononucleotide (NMN) by enzyme catalysis. And efficient synthesis of the NMN is realized. In order to further optimize the cost, an ATP (adenosine triphosphate) regeneration system is innovatively introduced in the process, so that the consumption of ATP in the reaction process is effectively reduced. On the basis, by combining an enzyme directed evolution technology, an immobilized enzyme technology and a continuous reactor design, the catalytic reaction efficiency and the product purity level are remarkably improved. The whole process has the characteristics of greenness and environmental protection, the reaction conditions are mild and controllable, the product yield stably reaches more than or equal to 85%, and the requirements of industrial large-scale production are completely met.

The invention provides a kit for DNA ligation reaction as well as a use method and application thereof. The kit comprises a first component and a second component, wherein the first component comprises a positively charged substance and a first buffer solution; the positively charged substance is selected from at least one of positively charged polypeptide, positively charged protein, positively charged synthetic macromolecule and positively charged glucan; the second component comprises a negatively charged substance and a second buffer solution; the negatively charged substance is selected from at least one of adenosine triphosphate, deoxyribonucleoside triphosphate, oligonucleotide and negatively charged polypeptide. The first component and the second component are arranged in the kit, when the first component and the second component are prepared into a reaction system, a condensate emulsion of hundreds of nanometers to several micrometers can be formed in situ, the condensate emulsion can spontaneously enrich all the components required by the reaction, and an emulsion matrix phase is a gel-like environment; and the ligation reaction efficiency and accuracy can be remarkably improved through the crowding effect between macromolecules.

The present invention provides a method for simultaneously determining nine components in a safflower pharmaceutical composition, belonging to the field of drug detection technology. The method comprises preparing a reference solution I by taking uridine, guanosine, adenosine, p-hydroxybenzoic acid glycoside, 5-hydroxymethylfurfural, syringin, and p-hydroxysafflower yellow A; preparing a reference solution II by taking p-hydroxycinnamic acid and kaempferol 3-O-rutinoside; preparing a test solution by taking the safflower pharmaceutical composition; and performing high-performance liquid chromatography on the reference solution I, the reference solution II, and the test solution, respectively. The contents of the nine components in the safflower pharmaceutical composition are then calculated using an external standard method. The present invention utilizes a method for determining the contents of nine chemical components in a safflower pharmaceutical composition by high-performance liquid chromatography at one time, thereby achieving control of multiple index components in the safflower pharmaceutical composition.

The invention provides chemically-modified adenosine triphosphate and a preparation method and application thereof.The chemically-modified adenosine and the chemically-modified adenosine triphosphate are synthesized by means of chemical synthesis, natural adenosine in mRNA is globally replaced with the chemically-modified adenosine, and the chemically-modified adenosine triphosphate is obtained. Or the mRNA is subjected to polyadenylation tailing modification, so that the translation efficiency and the stability of the target mRNA are enhanced, the immunogenicity is reduced, and a supplement and a new choice are provided for an mRNA modification technology.

The invention discloses a method and a kit for performing immunodetection on a target substance by using arterial blood, and belongs to the technical field of blood detection.The kit at least comprises a first component, a second component and a third component, the first component comprises luminous microspheres combined with a detection antibody 1 and a luminous microsphere diluent, and the luminous microsphere diluent contains 2-morpholinoethanesulfonic acid, KCl and NaHCO3; the second component comprises a biotin-labeled detection antibody 2 and a biotin diluent, and the biotin diluent contains dithiothreitol, CaCl2, adenosine and a mouse anti-human hemoglobin antibody; and a third component comprising a photosensitive microsphere bound with streptomycin. According to the scheme, the target substance can be subjected to immunodetection by utilizing arterial blood, meanwhile, the method has excellent functional sensitivity and detection range, and is not interfered by other substances in the arterial blood, so that rapid detection of all blood gas indexes and immune indexes of one tube of arterial blood is realized, and the method has important significance on clinical blood detection.

The invention discloses a preparation method of pyridoxine phosphate, a reaction system comprises a substrate pyridoxine, an enzyme composition and a coenzyme factor, the enzyme composition is pyridoxine kinase and polyphosphate kinase, and the coenzyme factor comprises a metal cofactor, ATP (adenosine triphosphate) and a phosphate donor. According to the invention, by introducing an adenosine triphosphate cyclic regeneration system, a process for in-vitro biosynthesis of pyridoxine phosphate through double-enzyme cascade with high stereoselectivity is obtained, so that the addition amount of ATP is reduced, and the production cost is remarkably reduced; according to the invention, the problems of complex steps, high-phosphorus wastewater generation and the like of a chemical synthesis method are avoided, and simple, efficient and environment-friendly synthesis of pyridoxine phosphate is realized; the method also has the advantages of mild reaction conditions, few generated impurities, easy product purification, high conversion efficiency and the like, and the yield can reach 99.6%.

The invention discloses a diphosphate and adenosine triphosphate synergistically modified calcium phosphate nanoparticle and a preparation method and application thereof, and belongs to the technical field of biological medicines.The preparation method comprises the following steps that a calcium chloride solution and a Tris buffer solution are dissolved in water, and a first solution is obtained; adding an HEPES buffer solution containing disodium hydrogen phosphate into water, then adding diphosphate and adenosine triphosphate, and uniformly stirring to obtain a second solution; dropwise adding the second solution into the first solution, uniformly stirring to obtain a mixed solution, and centrifuging, washing and drying the mixed solution to obtain calcium phosphate nanoparticles synergistically modified by diphosphate and adenosine triphosphate; through the synergistic effect of the diphosphate and the adenosine triphosphate, the size of the calcium phosphate nanoparticles is improved, and the Zeta potential of the calcium phosphate nanoparticles is adjusted, so that the circulation time of the calcium phosphate nanoparticles in vivo is prolonged, the delivery efficiency and the stability of the calcium phosphate nanoparticles are improved, the delivery efficiency of nucleic acid vaccines is improved, and the immune effect of the vaccines is enhanced.

The invention provides an electroplating cobalt plating solution for filling an X-type through hole of a printed circuit and application, the electroplating cobalt plating solution is used for the printed circuit and comprises cobalt sulfate heptahydrate, boric acid, sodium sulfate and sodium chloride, and an additive comprises one or more of adenine nucleoside, adenine, 6-mercaptopurine, 2-thioxanthine and 6-chloropurine. The high-quality filling effect is achieved, meanwhile, the average thickness increase of the surface cobalt is controlled to be smaller than 2 micrometers, the depression degree is controlled to be smaller than 2 micrometers, and the filling degree and the surface cobalt thickness control capacity are in the leading position in the similar invention and the existing production technology.