Method for separating and purifying four nucleoside chemical ingredients from trichosanthes bark

A technology for separation and purification of chemical components, applied in chemical instruments and methods, organic chemistry, chemical recycling, etc., can solve the problems of few types of nucleosides, cumbersome separation process steps, human and environmental hazards, etc., and achieve a short process cycle. , the effect of saving reagents, purity and efficiency

Active Publication Date: 2013-09-18
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE +2
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Problems solved by technology

[0005] The main problems of the above method are: the steps of the separation process are cumbersome, and sometimes chloroform is used as the extractant or eluent, which ...

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  • Method for separating and purifying four nucleoside chemical ingredients from trichosanthes bark
  • Method for separating and purifying four nucleoside chemical ingredients from trichosanthes bark
  • Method for separating and purifying four nucleoside chemical ingredients from trichosanthes bark

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Embodiment

[0034]Embodiment: the method for separating and purifying nucleoside chemical constituents from the skin of Chinese medicine Fructus Trichosanthes, its steps are:

[0035] (1) Preparation of the crude extract of Trichosanthes rind: after crushing the medicinal material of Trichosanthes trichosanthes, heat and reflux extraction with water for 4 times, combine the extracts, concentrate under reduced pressure to a relative density of about 1.2, and add 95 % ethanol until the concentration of ethanol reaches about 70%, let it stand for 72 h, filter, take the supernatant, and concentrate under reduced pressure to obtain the crude extract.

[0036] (2) Coarse separation with macroporous adsorption resin column: Dissolve the crude extract of Trichosanthes pipis in water, apply HPD826 macroporous adsorption resin column for chromatography, first elute with water, and then use ethanol with a concentration of about 10%- Elute with aqueous solution, and finally elute with ethanol-water s...

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Abstract

The invention relates to a method for separating and purifying four nucleoside chemical ingredients (namely adenine, guanosine, 6-iso inosine and adenosine) from traditional Chinese medicine trichosanthes bark. According to the method, four high-purity monomeric compounds, namely adenine, guanosine, 6-iso inosine and adenosine, are obtained from the trichosanthes bark through the following steps of: (1) preparing a crude trichosanthes bark extract; (2) carrying out crude separation by using a macroporous adsorption resin column; and (3) carrying out separation and purification by using semi-preparative high-performance liquid chromatography: carrying out separation and purification on obtained samples by using the semi-preparative high-performance liquid chromatography, wherein the mobile phase is methanol-water. The method disclosed by the invention has the advantages that the process flow is environment-friendly, the damage to the environment is not serious, and the comprehensive cost is low.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for separating and purifying four kinds of nucleoside chemical components (adenine, guanosine, 6-isosinosine and adenosine) from the peel of the traditional Chinese medicine Trichosanthes method. Background technique [0002] Trichosanthes peel is Cucurbitaceae Trichosanthes genus Trichosanthes ( Trichosanthes kirilowii Marim.) or Bilateral Trichiola ( Trichosanthes rosthornii Harms.), which is a commonly used traditional Chinese medicine, has the effects of nourishing the lungs and reducing phlegm, benefiting qi and widening the chest, etc. It is widely used clinically to treat cough with phlegm heat, phlegm turbid yellow thick, sore throat, chest pain, hematemesis, epistaxis, Constipation, carbuncle sore swelling and toxin embolism. Modern pharmacological experiments and clinical practice have shown that Trichosanthes peel has various pharmacological e...

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Application Information

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IPC IPC(8): C07H19/16C07H19/167C07H1/08
CPCY02P20/582
Inventor 李爱峰孙爱玲柳仁民
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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