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Method for detecting effective ingredient content of cordyceps capsules

An active ingredient, the technology of Cordyceps sinensis, which is applied in the detection field of the active ingredient content of Cordyceps sinensis capsules, can solve the problems of expensive operation of the instrument, high testing cost, complexity, etc., and achieve the effect of accurate and sensitive detection results and easy operation

Inactive Publication Date: 2011-07-20
DALIAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods involve expensive instruments, complex operations, and high testing costs, so new detection methods need to be developed

Method used

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  • Method for detecting effective ingredient content of cordyceps capsules
  • Method for detecting effective ingredient content of cordyceps capsules

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preparation of MWNT / Au modified electrode:

[0019] ①Purification and functionalization of MWNTs: MWNTs were purified by ultrasonication in concentrated hydrochloric acid for 7.5 hours, and the purified MWNTs were refluxed with concentrated nitric acid at 140°C for 8 hours, washed with water twice to neutrality, dried at 100°C and ground into powder, ready for use .

[0020] ② Electrode preparation: Weigh 2.0 mg of the functionalized MWNT powder obtained in 1.2 and ultrasonically disperse it in DMF. The amount of MWNT is 1.0 mg / ml to form a black suspension for later use. The gold electrode was first polished to a mirror surface with 0.05 μm Al2O3 polishing powder, and then ultrasonicated in distilled water, a solution of nitric acid and water at a volume ratio of 1:1, absolute ethanol and distilled water for three minutes. Use a micro-injector to take the suspension and drop it on the surface of the gold electrode, and dry it under an infrared lamp to obtain the MW...

Embodiment 2

[0024] Linear range, reproducibility and stability

[0025] In the test, under the optimized experimental conditions, the polarization currents to the following different concentrations of adenosine were sequentially detected: 1.0×10 -4 mol L -1 , 1.0×10 -2 mol L -1 , 1.0×10 -3 mol L -1 , 1.0×10 -4 mol L -1 , 1.0×10 -5 mol L -1 , 1.0×10 -6 mol L -1 , 1.0×10 -7 mol L -1 , 1.0×10 -8 mol L -1 , 1.0×10 -9 mol L -1 . The highest oxidation peak current of adenosine and its concentration are at 1.0×10 -1 mol L -1 ~1.0×10 -8 mol L -1 There is a good linear relationship in the range of , the linear equation:

[0026] A(mA)=0.0431-0.0046C(mol·L -1 ), correlation coefficient R 2 = 0.9947.

[0027] The modified electrode was stored at room temperature for 2 weeks, and the oxidation peak current was basically unchanged. For 1.0×10 -4 mol L -1 adenosine solution, the relative standard deviation (RSD) of 5 consecutive parallel determinations was 2.8%. The detectio...

Embodiment 3

[0029] Performance test of carbon nanotube modified electrodes with different concentrations

[0030] Experiments have found that different concentrations of carbon nanotubes in the range of 0.5 to 2.5 mg / ml are modified on the gold electrode, and reduction peaks of different shapes will appear. When the concentration of carbon nanotubes is 1 mg / ml, the peak current is the largest and the peak shape is Best and most reproducible. During the experiment, we took 0.5mg / ml, 1mg / ml, 1.5mg / ml, 2.0mg / ml, and 2.5mg / ml of MWNT-DMF suspensions for modification. After testing, only when the concentration was 1mg / ml ml, there is a good reduction peak, the results are as attached figure 1 shown.

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Abstract

The invention discloses a method for detecting effective ingredient content of cordyceps capsules. A multi-wall nano tube / Au (MWNT) / Au modified electrode and a stripping voltammetry are used for detection. The preparation of the modified electrode comprises the following steps of: (1) performing ultrasonic purification on MWNT in concentrated hydrochloric acid for 7.5 hours, refluxing the purified MWNT for 8 hours at the temperature of 140 DEG C by adding concentrated nitric acid, performing secondary washing till neutrality, drying the washed product at the temperature of 100 DEG C, and grinding the dried product into powder, namely functional MWNT powder; and (2) ultrasonically dispersing the functional MWNT powder obtained in the step (1) into dimethyl formamide (DMF) to form black suspension, dripping the suspension on the surface of a gold electrode by using a trace sample injector, and drying the suspension under an infrared lamp to obtain the MWNT / Au modified electrode. The method for detecting the content of adenosine serving as the effective ingredient of the cordyceps capsules is economic and simple and convenient in operation, and the detection result is accurate and sensitive.

Description

technical field [0001] The invention relates to a method for detecting the content of active ingredients by stripping voltammetry, in particular to a method for detecting adenosine concentration by using a modified electrode. Background technique [0002] Cordyceps sinensis is a precious traditional Chinese medicine in China. It is a complex of the larvae and larvae of the fungus Cordyceps sinensis Sace. Due to the high price, there are often counterfeit Cordyceps chinensis on the market. Its source is Cordycepshawkesii Gray, an ergot fungus. It is an ascomycete that parasitizes the larvae of Lepidoptera insects and grows from the body and head of the insect. Subseat composition. Genuine Cordyceps sinensis contains nucleosides, polysaccharides, sterols, amino acids and other active ingredients. Traditional Chinese medicine believes that it has the effects of nourishing the lungs and kidneys, stopping bleeding and reducing phlegm. In addition, it also has various pharmacolo...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/42G01N27/416
Inventor 申贵隽陈乐玥申远孙杨
Owner DALIAN UNIVERSITY
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